Lesion Sensing during Initial Binding by Yeast XPC/Rad4: Toward Predicting Resistance to Nucleotide Excision Repair
Abstract
Nucleotide excision repair (NER) excises a variety of environmentally derived DNA lesions. However, NER efficiencies for structurally different DNA lesions can vary by orders of magnitude; yet the origin of this variance is poorly understood. Our goal is to develop computational strategies that predict and identify the most hazardous, repair-resistant lesions from the plethora of such adducts. In the present work, we are focusing on lesion recognition by the xeroderma pigmentosum C protein complex (XPC), the first and required step for the subsequent assembly of factors needed to produce successful NER. We have performed molecular dynamics simulations to characterize the initial binding of Rad4, the yeast orthologue of human XPC, to a library of 10 different lesion-containing DNA duplexes derived from environmental carcinogens. These vary in lesion chemical structures and conformations in duplex DNA and exhibit a wide range of relative NER efficiencies from repair resistant to highly susceptible. We have determined a promising set of structural descriptors that characterize initial binding of Rad4 to lesions that are resistant to NER. Key initial binding requirements for successful recognition are absent in the repair-resistant cases: There is little or no duplex unwinding, very limited interaction between the β-hairpin domain 2 ofmore »
- Authors:
-
- New York Univ. (NYU), NY (United States)
- New York Univ. (NYU), NY (United States); New York Univ. Shanghai (China) NYU-ECNU Center for Computational Chemistry
- Publication Date:
- Research Org.:
- Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States). National Energy Research Scientific Computing Center (NERSC)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1543617
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Chemical Research in Toxicology
- Additional Journal Information:
- Journal Volume: 31; Journal Issue: 11; Journal ID: ISSN 0893-228X
- Publisher:
- American Chemical Society (ACS)
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 60 APPLIED LIFE SCIENCES; Pharmacology & Pharmacy; Chemistry; Toxicology
Citation Formats
Mu, Hong, Zhang, Yingkai, Geacintov, Nicholas E., and Broyde, Suse. Lesion Sensing during Initial Binding by Yeast XPC/Rad4: Toward Predicting Resistance to Nucleotide Excision Repair. United States: N. p., 2018.
Web. doi:10.1021/acs.chemrestox.8b00231.
Mu, Hong, Zhang, Yingkai, Geacintov, Nicholas E., & Broyde, Suse. Lesion Sensing during Initial Binding by Yeast XPC/Rad4: Toward Predicting Resistance to Nucleotide Excision Repair. United States. https://doi.org/10.1021/acs.chemrestox.8b00231
Mu, Hong, Zhang, Yingkai, Geacintov, Nicholas E., and Broyde, Suse. Thu .
"Lesion Sensing during Initial Binding by Yeast XPC/Rad4: Toward Predicting Resistance to Nucleotide Excision Repair". United States. https://doi.org/10.1021/acs.chemrestox.8b00231. https://www.osti.gov/servlets/purl/1543617.
@article{osti_1543617,
title = {Lesion Sensing during Initial Binding by Yeast XPC/Rad4: Toward Predicting Resistance to Nucleotide Excision Repair},
author = {Mu, Hong and Zhang, Yingkai and Geacintov, Nicholas E. and Broyde, Suse},
abstractNote = {Nucleotide excision repair (NER) excises a variety of environmentally derived DNA lesions. However, NER efficiencies for structurally different DNA lesions can vary by orders of magnitude; yet the origin of this variance is poorly understood. Our goal is to develop computational strategies that predict and identify the most hazardous, repair-resistant lesions from the plethora of such adducts. In the present work, we are focusing on lesion recognition by the xeroderma pigmentosum C protein complex (XPC), the first and required step for the subsequent assembly of factors needed to produce successful NER. We have performed molecular dynamics simulations to characterize the initial binding of Rad4, the yeast orthologue of human XPC, to a library of 10 different lesion-containing DNA duplexes derived from environmental carcinogens. These vary in lesion chemical structures and conformations in duplex DNA and exhibit a wide range of relative NER efficiencies from repair resistant to highly susceptible. We have determined a promising set of structural descriptors that characterize initial binding of Rad4 to lesions that are resistant to NER. Key initial binding requirements for successful recognition are absent in the repair-resistant cases: There is little or no duplex unwinding, very limited interaction between the β-hairpin domain 2 of Rad4 and the minor groove of the lesion-containing duplex, and no conformational capture of a base on the lesion partner strand. By contrast, these key binding features are present to different degrees in NER susceptible lesions and correlate to their relative NER efficiencies. Furthermore, we have gained molecular understanding of Rad4 initial binding as determined by the lesion structures in duplex DNA and how the initial binding relates to the repair efficiencies. The development of a computational strategy for identifying NER-resistant lesions is grounded in this molecular understanding of the lesion recognition mechanism.},
doi = {10.1021/acs.chemrestox.8b00231},
journal = {Chemical Research in Toxicology},
number = 11,
volume = 31,
place = {United States},
year = {Thu Oct 04 00:00:00 EDT 2018},
month = {Thu Oct 04 00:00:00 EDT 2018}
}
Web of Science
Figures / Tables:
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