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Title: Directed in vitro evolution of bacterial expansin BsEXLX1 for higher cellulose binding and its consequences for plant cell wall‐loosening activities

Authors:
 [1];  [1]
  1. Huck Institutes of the Life Sciences The Pennsylvania State University University Park PA USA, Department of Biology The Pennsylvania State University University Park PA USA
Publication Date:
Sponsoring Org.:
USDOE Office of Science (SC), Basic Energy Sciences (BES) (SC-22)
OSTI Identifier:
1543169
Grant/Contract Number:  
DE‐FG02‐84ER13179
Resource Type:
Publisher's Accepted Manuscript
Journal Name:
FEBS Letters
Additional Journal Information:
Journal Name: FEBS Letters Journal Volume: 593 Journal Issue: 18; Journal ID: ISSN 0014-5793
Publisher:
Wiley Blackwell (John Wiley & Sons)
Country of Publication:
Netherlands
Language:
English

Citation Formats

Hepler, Nathan K., and Cosgrove, Daniel J. Directed in vitro evolution of bacterial expansin BsEXLX1 for higher cellulose binding and its consequences for plant cell wall‐loosening activities. Netherlands: N. p., 2019. Web. doi:10.1002/1873-3468.13528.
Hepler, Nathan K., & Cosgrove, Daniel J. Directed in vitro evolution of bacterial expansin BsEXLX1 for higher cellulose binding and its consequences for plant cell wall‐loosening activities. Netherlands. doi:10.1002/1873-3468.13528.
Hepler, Nathan K., and Cosgrove, Daniel J. Wed . "Directed in vitro evolution of bacterial expansin BsEXLX1 for higher cellulose binding and its consequences for plant cell wall‐loosening activities". Netherlands. doi:10.1002/1873-3468.13528.
@article{osti_1543169,
title = {Directed in vitro evolution of bacterial expansin BsEXLX1 for higher cellulose binding and its consequences for plant cell wall‐loosening activities},
author = {Hepler, Nathan K. and Cosgrove, Daniel J.},
abstractNote = {},
doi = {10.1002/1873-3468.13528},
journal = {FEBS Letters},
number = 18,
volume = 593,
place = {Netherlands},
year = {2019},
month = {7}
}

Journal Article:
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This content will become publicly available on July 18, 2020
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Works referenced in this record:

Bacterial expansins and related proteins from the world of microbes
journal, April 2015

  • Georgelis, Nikolaos; Nikolaidis, Nikolas; Cosgrove, Daniel J.
  • Applied Microbiology and Biotechnology, Vol. 99, Issue 9
  • DOI: 10.1007/s00253-015-6534-0

Microbial Expansins
journal, September 2017


Expansin Mode of Action on Cell Walls (Analysis of Wall Hydrolysis, Stress Relaxation, and Binding)
journal, January 1995

  • McQueen-Mason, S. J.; Cosgrove, D. J.
  • Plant Physiology, Vol. 107, Issue 1
  • DOI: 10.1104/pp.107.1.87

Xylo- and cello-oligosaccharide oxidation by gluco-oligosaccharide oxidase from Sarocladium strictum and variants with reduced substrate inhibition
journal, January 2013

  • Vuong, Thu V.; Vesterinen, Arja-Helena; Foumani, Maryam
  • Biotechnology for Biofuels, Vol. 6, Issue 1
  • DOI: 10.1186/1754-6834-6-148

Automated comparative protein structure modeling with SWISS-MODEL and Swiss-PdbViewer: A historical perspective
journal, June 2009

  • Guex, Nicolas; Peitsch, Manuel C.; Schwede, Torsten
  • ELECTROPHORESIS, Vol. 30, Issue S1
  • DOI: 10.1002/elps.200900140

Characteristics of the binding of a bacterial expansin ( Bs EXLX1) to microcrystalline cellulose
journal, September 2012

  • Kim, In Jung; Ko, Hyeok-Jin; Kim, Tae-Wan
  • Biotechnology and Bioengineering, Vol. 110, Issue 2
  • DOI: 10.1002/bit.24719

PcExl1 a Novel Acid Expansin-Like Protein from the Plant Pathogen Pectobacterium carotovorum, Binds Cell Walls Differently to BsEXLX1
journal, April 2014


Binding characteristics and synergistic effects of bacterial expansins on cellulosic and hemicellulosic substrates
journal, January 2015


Structure-Function Analysis of the Bacterial Expansin EXLX1
journal, March 2011

  • Georgelis, Nikolaos; Tabuchi, Akira; Nikolaidis, Nikolas
  • Journal of Biological Chemistry, Vol. 286, Issue 19
  • DOI: 10.1074/jbc.M111.225037

Sensitivity-enhanced solid-state NMR detection of expansin's target in plant cell walls
journal, September 2013

  • Wang, T.; Park, Y. B.; Caporini, M. A.
  • Proceedings of the National Academy of Sciences, Vol. 110, Issue 41
  • DOI: 10.1073/pnas.1316290110

Crystal structure and activities of EXPB1 (Zea m 1), a beta-expansin and group-1 pollen allergen from maize
journal, September 2006

  • Yennawar, N. H.; Li, L. -C.; Dudzinski, D. M.
  • Proceedings of the National Academy of Sciences, Vol. 103, Issue 40
  • DOI: 10.1073/pnas.0605979103

Molecular Characterization of A Novel Effector Expansin-like Protein from Heterodera avenae that Induces Cell Death in Nicotiana benthamiana
journal, November 2016

  • Liu, Jing; Peng, Huan; Cui, Jiangkuan
  • Scientific Reports, Vol. 6, Issue 1
  • DOI: 10.1038/srep35677

Carbohydrate-binding modules: fine-tuning polysaccharide recognition
journal, September 2004

  • Boraston, Alisdair B.; Bolam, David N.; Gilbert, Harry J.
  • Biochemical Journal, Vol. 382, Issue 3
  • DOI: 10.1042/BJ20040892

The Target of β-Expansin EXPB1 in Maize Cell Walls from Binding and Solid-State NMR Studies
journal, October 2016

  • Wang, Tuo; Chen, Yuning; Tabuchi, Akira
  • Plant Physiology, Vol. 172, Issue 4
  • DOI: 10.1104/pp.16.01311

Synergistic proteins for the enhanced enzymatic hydrolysis of cellulose by cellulase
journal, August 2014

  • Kim, In Jung; Lee, Hee Jin; Choi, In-Geol
  • Applied Microbiology and Biotechnology, Vol. 98, Issue 20
  • DOI: 10.1007/s00253-014-6001-3

Plant-like bacterial expansins play contrasting roles in two tomato vascular pathogens: Bacterial expansins modify plant interactions
journal, December 2017

  • Tancos, Matthew A.; Lowe-Power, Tiffany M.; Peritore-Galve, F. Christopher
  • Molecular Plant Pathology, Vol. 19, Issue 5
  • DOI: 10.1111/mpp.12611

Matrix solubilization and cell wall weakening by β-expansin (group-1 allergen) from maize pollen: Matrix solubilization by pollen β-expansin
journal, August 2011


Biochemical analysis of expansin-like proteins from microbes
journal, January 2014


Disruption of hydrogen bonding between plant cell wall polymers by proteins that induce wall extension.
journal, July 1994

  • McQueen-Mason, S.; Cosgrove, D. J.
  • Proceedings of the National Academy of Sciences, Vol. 91, Issue 14
  • DOI: 10.1073/pnas.91.14.6574

Structural basis for entropy-driven cellulose binding by a type-A cellulose-binding module (CBM) and bacterial expansin
journal, August 2012

  • Georgelis, N.; Yennawar, N. H.; Cosgrove, D. J.
  • Proceedings of the National Academy of Sciences, Vol. 109, Issue 37
  • DOI: 10.1073/pnas.1213200109

Functional characterization of a bacterial expansin from Bacillus subtilis for enhanced enzymatic hydrolysis of cellulose
journal, April 2009

  • Kim, Eun Sil; Lee, Hee Jin; Bang, Won-Gi
  • Biotechnology and Bioengineering, Vol. 102, Issue 5
  • DOI: 10.1002/bit.22193

Plant expansins: diversity and interactions with plant cell walls
journal, June 2015


Directed Evolution: Bringing New Chemistry to Life
journal, November 2017


Purification and Characterization of Four β-Expansins (Zea m 1 Isoforms) from Maize Pollen
journal, July 2003

  • Li, Lian-Chao; Bedinger, Patricia A.; Volk, Carol
  • Plant Physiology, Vol. 132, Issue 4
  • DOI: 10.1104/pp.103.020024

Molecular dynamics of the Bacillus subtilis expansin EXLX1: interaction with substrates and structural basis of the lack of activity of mutants
journal, January 2016

  • Silveira, Rodrigo L.; Skaf, Munir S.
  • Physical Chemistry Chemical Physics, Vol. 18, Issue 5
  • DOI: 10.1039/C5CP06674C

The role of conserved tryptophan residues in the interaction of a bacterial cellulose binding domain with its ligand
journal, January 1993


Crystal structure and activity of Bacillus subtilis YoaJ (EXLX1), a bacterial expansin that promotes root colonization
journal, October 2008

  • Kerff, F.; Amoroso, A.; Herman, R.
  • Proceedings of the National Academy of Sciences, Vol. 105, Issue 44
  • DOI: 10.1073/pnas.0809382105

Affinity maturation generates greatly improved xyloglucan-specific carbohydrate binding modules
journal, January 2009

  • von Schantz, Laura; Gullfot, Fredrika; Scheer, Sebastian
  • BMC Biotechnology, Vol. 9, Issue 1
  • DOI: 10.1186/1472-6750-9-92

Loosening of plant cell walls by expansins
journal, September 2000