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Title: Diversion of Carbon Flux from Sugars to Lipids Improves the Growth of an Arabidopsis Starchless Mutant

Abstract

Inactivation of ADP-glucose pyrophosphorylase1 (ADG1) causes a starchless phenotype in Arabidopsis. Mutants defective in ADG1 show severe growth retardation in day/night conditions but exhibit similar growth to wild type under continuous light, implying that starch plays an important role in supporting respiration, metabolism and growth at night. In addition to carbohydrates, lipids and proteins can serve as alternative respiratory substrates for the energy production in mature plants. To test the role of lipids in plant growth, we generated transgenic plants overexpressing phospholipid:diacylglycerol acyltransferase1 (PDAT1) in adg1. We found that PDAT1 overexpression caused an increase in both fatty acid synthesis and turnover and increased the accumulation of triacylglycerol (TAG) at the expense of sugars, and enhanced the growth of adg1. We demonstrated that unlike sugars, which were metabolized within a few hours of darkness, TAG breakdown was slow, occurring throughout the entire dark period. The slow pace of TAG hydrolysis provided a sustained supply of fatty acids for energy production, thereby alleviating energy deficiency at night and thereby improving the growth of the starchless mutants. We conclude that lipids can contribute to plant growth by providing a constant supply of fatty acids as an alternative energy source in mature starchless mutantmore » plants.« less

Authors:
; ORCiD logo; ORCiD logo; ; ; ORCiD logo
Publication Date:
Research Org.:
Center for Advanced Bioenergy and Bioproducts Innovation (CABBI), Urbana, IL (United States); Brookhaven National Lab. (BNL), Upton, NY (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Biological and Environmental Research (BER) (SC-23); USDOE Office of Science (SC), Basic Energy Sciences (BES) (SC-22). Chemical Sciences, Geosciences & Biosciences Division
OSTI Identifier:
1543017
Alternate Identifier(s):
OSTI ID: 1546074; OSTI ID: 1556883
Report Number(s):
BNL-211945-2019-JAAM
Journal ID: ISSN 2223-7747; PLANCD; PII: plants8070229
Grant/Contract Number:  
SC0018420 and DE-SC0012704; SC0018420; SC0012704
Resource Type:
Published Article
Journal Name:
Plants
Additional Journal Information:
Journal Name: Plants Journal Volume: 8 Journal Issue: 7; Journal ID: ISSN 2223-7747
Publisher:
MDPI AG
Country of Publication:
Netherlands
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; starch; sugars; lipids; triacylglycerol; phospholipid:diacylglycerol acyltransferase1; phospholipid; diacylglycerol acyltransferase1

Citation Formats

Fan, Jilian, Zhou, Chao, Yu, Linhui, Li, Ping, Shanklin, John, and Xu, Changcheng. Diversion of Carbon Flux from Sugars to Lipids Improves the Growth of an Arabidopsis Starchless Mutant. Netherlands: N. p., 2019. Web. doi:10.3390/plants8070229.
Fan, Jilian, Zhou, Chao, Yu, Linhui, Li, Ping, Shanklin, John, & Xu, Changcheng. Diversion of Carbon Flux from Sugars to Lipids Improves the Growth of an Arabidopsis Starchless Mutant. Netherlands. doi:10.3390/plants8070229.
Fan, Jilian, Zhou, Chao, Yu, Linhui, Li, Ping, Shanklin, John, and Xu, Changcheng. Wed . "Diversion of Carbon Flux from Sugars to Lipids Improves the Growth of an Arabidopsis Starchless Mutant". Netherlands. doi:10.3390/plants8070229.
@article{osti_1543017,
title = {Diversion of Carbon Flux from Sugars to Lipids Improves the Growth of an Arabidopsis Starchless Mutant},
author = {Fan, Jilian and Zhou, Chao and Yu, Linhui and Li, Ping and Shanklin, John and Xu, Changcheng},
abstractNote = {Inactivation of ADP-glucose pyrophosphorylase1 (ADG1) causes a starchless phenotype in Arabidopsis. Mutants defective in ADG1 show severe growth retardation in day/night conditions but exhibit similar growth to wild type under continuous light, implying that starch plays an important role in supporting respiration, metabolism and growth at night. In addition to carbohydrates, lipids and proteins can serve as alternative respiratory substrates for the energy production in mature plants. To test the role of lipids in plant growth, we generated transgenic plants overexpressing phospholipid:diacylglycerol acyltransferase1 (PDAT1) in adg1. We found that PDAT1 overexpression caused an increase in both fatty acid synthesis and turnover and increased the accumulation of triacylglycerol (TAG) at the expense of sugars, and enhanced the growth of adg1. We demonstrated that unlike sugars, which were metabolized within a few hours of darkness, TAG breakdown was slow, occurring throughout the entire dark period. The slow pace of TAG hydrolysis provided a sustained supply of fatty acids for energy production, thereby alleviating energy deficiency at night and thereby improving the growth of the starchless mutants. We conclude that lipids can contribute to plant growth by providing a constant supply of fatty acids as an alternative energy source in mature starchless mutant plants.},
doi = {10.3390/plants8070229},
journal = {Plants},
number = 7,
volume = 8,
place = {Netherlands},
year = {2019},
month = {7}
}

Journal Article:
Free Publicly Available Full Text
Publisher's Version of Record
DOI: 10.3390/plants8070229

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