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Title: Biosystems design by directed evolution

Abstract

Through iterative rounds of genetic diversification and screening or selection, directed evolution has been widely used to engineer relatively simple biosystems such as nucleic acids and proteins with desired functions. In addition, directed evolution has played an important role in engineering more complex biosystems such as pathways and genomes. Since 2013, directed evolution has been further explored for biosystems design with numerous newly developed techniques that have enabled design and engineering of proteins, pathways, and genomes in a much more effective manner. In this review, we will highlight the abiotic biotransformations arisen from directed evolution and novel strategies for continuous evolution in vivo and ultrahigh-throughput screening. We will also discuss the future challenges and opportunities of applying directed evolution for biosystems design.

Authors:
 [1];  [2]; ORCiD logo [1]
  1. Univ. of Illinois, Urbana-Champaign, IL (United States)
  2. Univ. of Illinois, Urbana-Champaign, IL (United States); Jiangnan Univ., Wuxi (China)
Publication Date:
Research Org.:
Center for Advanced Bioenergy and Bioproducts Innovation (CABBI), Urbana, IL (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Biological and Environmental Research (BER)
OSTI Identifier:
1542919
Alternate Identifier(s):
OSTI ID: 1542484
Grant/Contract Number:  
SC0018420; DE‐SC0018260; DE‐SC0018420
Resource Type:
Accepted Manuscript
Journal Name:
AIChE Journal
Additional Journal Information:
Journal Volume: 66; Journal Issue: 3; Journal ID: ISSN 0001-1541
Publisher:
American Institute of Chemical Engineers
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; directed evolution, genome engineering, pathway engineering, protein engineering, synthetic biology

Citation Formats

Wang, Yajie, Yu, Xiaowei, and Zhao, Huimin. Biosystems design by directed evolution. United States: N. p., 2019. Web. https://doi.org/10.1002/aic.16716.
Wang, Yajie, Yu, Xiaowei, & Zhao, Huimin. Biosystems design by directed evolution. United States. https://doi.org/10.1002/aic.16716
Wang, Yajie, Yu, Xiaowei, and Zhao, Huimin. Fri . "Biosystems design by directed evolution". United States. https://doi.org/10.1002/aic.16716. https://www.osti.gov/servlets/purl/1542919.
@article{osti_1542919,
title = {Biosystems design by directed evolution},
author = {Wang, Yajie and Yu, Xiaowei and Zhao, Huimin},
abstractNote = {Through iterative rounds of genetic diversification and screening or selection, directed evolution has been widely used to engineer relatively simple biosystems such as nucleic acids and proteins with desired functions. In addition, directed evolution has played an important role in engineering more complex biosystems such as pathways and genomes. Since 2013, directed evolution has been further explored for biosystems design with numerous newly developed techniques that have enabled design and engineering of proteins, pathways, and genomes in a much more effective manner. In this review, we will highlight the abiotic biotransformations arisen from directed evolution and novel strategies for continuous evolution in vivo and ultrahigh-throughput screening. We will also discuss the future challenges and opportunities of applying directed evolution for biosystems design.},
doi = {10.1002/aic.16716},
journal = {AIChE Journal},
number = 3,
volume = 66,
place = {United States},
year = {2019},
month = {7}
}

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