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Title: Surface chemical defence of the eelgrass Zostera marina against microbial foulers

Abstract

Plants rely on both mechanical and chemical defence mechanisms to protect their surfaces against microorganisms. The recently completed genome of the eelgrass Zostera marina, a marine angiosperm with fundamental importance for coastal ecosystems, showed that its re-adaptation from land to the sea has led to the loss of essential genes (for chemical communication and defence) and structural features (stomata and thick cuticle) that are typical of terrestrial plants. This study was designed to understand the molecular nature of surface protection and fouling-control strategy of eelgrass against marine epiphytic yeasts. Different surface extraction methods and comparative metabolomics by tandem mass spectrometry (LC-MS/MS) were used for targeted and untargeted identification of the metabolite profiles of the leaf surface and the whole tissue extracts. Desorption electrospray ionization-imaging mass spectrometry (DESI-IMS) coupled with traditional bioassays revealed, for the first time, the unique spatial distribution of the eelgrass surface-associated phenolics and fatty acids, as well as their differential bioactivity against the growth and settlement of epiphytic yeasts. This study provides insights into the complex chemical defence system of the eelgrass leaf surface. It suggests that surface-associated metabolites modulate biotic interactions and provide chemical defence and structural protection to eelgrass in its marine environment.

Authors:
 [1];  [1];  [2];  [2];  [1]
  1. GEOMAR Helmholtz Centre for Ocean Research Kiel, Kiel (Germany). GEOMAR Centre for Marine Biotechnology, Research Unit Marine Natural Products Chemistry
  2. GEOMAR Helmholtz Centre for Ocean Research Kiel, Kiel (Germany). Research Unit Marine Benthic Ecology
Publication Date:
Research Org.:
Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States). National Energy Research Scientific Computing Center (NERSC)
Sponsoring Org.:
USDOE
OSTI Identifier:
1542435
Resource Type:
Accepted Manuscript
Journal Name:
Scientific Reports
Additional Journal Information:
Journal Volume: 9; Journal Issue: 1; Journal ID: ISSN 2045-2322
Publisher:
Nature Publishing Group
Country of Publication:
United States
Language:
English

Citation Formats

Papazian, Stefano, Parrot, Delphine, Burýšková, Barbora, Weinberger, Florian, and Tasdemir, Deniz. Surface chemical defence of the eelgrass Zostera marina against microbial foulers. United States: N. p., 2019. Web. doi:10.1038/s41598-019-39212-3.
Papazian, Stefano, Parrot, Delphine, Burýšková, Barbora, Weinberger, Florian, & Tasdemir, Deniz. Surface chemical defence of the eelgrass Zostera marina against microbial foulers. United States. doi:10.1038/s41598-019-39212-3.
Papazian, Stefano, Parrot, Delphine, Burýšková, Barbora, Weinberger, Florian, and Tasdemir, Deniz. Tue . "Surface chemical defence of the eelgrass Zostera marina against microbial foulers". United States. doi:10.1038/s41598-019-39212-3. https://www.osti.gov/servlets/purl/1542435.
@article{osti_1542435,
title = {Surface chemical defence of the eelgrass Zostera marina against microbial foulers},
author = {Papazian, Stefano and Parrot, Delphine and Burýšková, Barbora and Weinberger, Florian and Tasdemir, Deniz},
abstractNote = {Plants rely on both mechanical and chemical defence mechanisms to protect their surfaces against microorganisms. The recently completed genome of the eelgrass Zostera marina, a marine angiosperm with fundamental importance for coastal ecosystems, showed that its re-adaptation from land to the sea has led to the loss of essential genes (for chemical communication and defence) and structural features (stomata and thick cuticle) that are typical of terrestrial plants. This study was designed to understand the molecular nature of surface protection and fouling-control strategy of eelgrass against marine epiphytic yeasts. Different surface extraction methods and comparative metabolomics by tandem mass spectrometry (LC-MS/MS) were used for targeted and untargeted identification of the metabolite profiles of the leaf surface and the whole tissue extracts. Desorption electrospray ionization-imaging mass spectrometry (DESI-IMS) coupled with traditional bioassays revealed, for the first time, the unique spatial distribution of the eelgrass surface-associated phenolics and fatty acids, as well as their differential bioactivity against the growth and settlement of epiphytic yeasts. This study provides insights into the complex chemical defence system of the eelgrass leaf surface. It suggests that surface-associated metabolites modulate biotic interactions and provide chemical defence and structural protection to eelgrass in its marine environment.},
doi = {10.1038/s41598-019-39212-3},
journal = {Scientific Reports},
number = 1,
volume = 9,
place = {United States},
year = {2019},
month = {2}
}

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Works referenced in this record:

Mass Spectrometry Sampling Under Ambient Conditions with Desorption Electrospray Ionization
journal, October 2004