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Title: Xyloglucan Endotransglucosylase-Hydrolase30 negatively affects salt tolerance in Arabidopsis

Abstract

Plants have evolved various strategies to sense and respond to saline environments that severely reduce plant growth and limit agricultural productivity. Alteration to the cell wall is one strategy that helps plants adapt to salt stress. However, we do not fully understand the physiological significance of how cell wall components respond to salt stress. Here, we show that expression of XTH30, encoding a xyloglucan endotransglucosylase/hydrolase, is strongly up-regulated in response to salt stress in Arabidopsis thaliana. Loss-of-function in XTH30 leads to increased salt tolerance and overexpression of XTH30 results in salt hypersensitivity. XTH30 is located in the plasma membrane and highly expressed in the root, flower, stem, and etiolated hypocotyl. The NaCl-induced increase in XLFG (xyloglucan-derived (XyG) oligosaccharide) of the wild type is partly blocked in xth30 mutants. Loss-of-function in XTH30 slows down the decrease of crystalline cellulose content and the depolymerization of microtubules caused by salt stress. Moreover, less Na+ accumulation in shoot and H2O2 content are found in xth30 mutants in response to salt stress. Taken together, these results indicate that XTH30 modulates XyG side chains with altered abundance of XLFG, the cellulose synthesis and cortical microtubule stability, and then negatively affects salt tolerance.

Authors:
 [1];  [1];  [1];  [1];  [1];  [2];  [3];  [2];  [2];  [2];  [1];  [4];  [5];  [4]
  1. Nanjing Agricultural Univ., Nanjing, Jiangsu (China). College of Life Sciences
  2. Joint BioEnergy Inst. (JBEI), Emeryville, CA (United States); Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
  3. Chinese Academy of Sciences (CAS), Guangzhou (China)
  4. Nanjing Agricultural Univ., Nanjing, Jiangsu (China). College of Life Sciences, and State Key Lab. of Crop Genetics and Germplasm Enhancement
  5. Nanjing Agricultural Univ., Nanjing, Jiangsu (China). State Key Lab. of Crop Genetics and Germplasm Enhancement, and Key Lab. of Biology and Germplasm Enhancement of Horticultural Crops in East China, Ministry of Agriculture
Publication Date:
Research Org.:
Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Biological and Environmental Research (BER)
OSTI Identifier:
1542421
Grant/Contract Number:  
AC02-05CH11231
Resource Type:
Accepted Manuscript
Journal Name:
Journal of Experimental Botany
Additional Journal Information:
Journal Volume: 70; Journal Issue: 19; Journal ID: ISSN 0022-0957
Publisher:
Oxford University Press
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; Arabidopsis; cellulose; microtubule; salt stress; XLFG; XTH30; xyloglucan

Citation Formats

Yan, Jingwei, Huang, Yun, He, Huan, Han, Tong, Di, Pengcheng, Sechet, Julien, Fang, Lin, Liang, Yan, Scheller, Henrik Vibe, Mortimer, Jenny C., Ni, Lan, Jiang, Mingyi, Hou, Xilin, and Zhang, Aying. Xyloglucan Endotransglucosylase-Hydrolase30 negatively affects salt tolerance in Arabidopsis. United States: N. p., 2019. Web. doi:10.1093/jxb/erz311.
Yan, Jingwei, Huang, Yun, He, Huan, Han, Tong, Di, Pengcheng, Sechet, Julien, Fang, Lin, Liang, Yan, Scheller, Henrik Vibe, Mortimer, Jenny C., Ni, Lan, Jiang, Mingyi, Hou, Xilin, & Zhang, Aying. Xyloglucan Endotransglucosylase-Hydrolase30 negatively affects salt tolerance in Arabidopsis. United States. doi:https://doi.org/10.1093/jxb/erz311
Yan, Jingwei, Huang, Yun, He, Huan, Han, Tong, Di, Pengcheng, Sechet, Julien, Fang, Lin, Liang, Yan, Scheller, Henrik Vibe, Mortimer, Jenny C., Ni, Lan, Jiang, Mingyi, Hou, Xilin, and Zhang, Aying. Sat . "Xyloglucan Endotransglucosylase-Hydrolase30 negatively affects salt tolerance in Arabidopsis". United States. doi:https://doi.org/10.1093/jxb/erz311. https://www.osti.gov/servlets/purl/1542421.
@article{osti_1542421,
title = {Xyloglucan Endotransglucosylase-Hydrolase30 negatively affects salt tolerance in Arabidopsis},
author = {Yan, Jingwei and Huang, Yun and He, Huan and Han, Tong and Di, Pengcheng and Sechet, Julien and Fang, Lin and Liang, Yan and Scheller, Henrik Vibe and Mortimer, Jenny C. and Ni, Lan and Jiang, Mingyi and Hou, Xilin and Zhang, Aying},
abstractNote = {Plants have evolved various strategies to sense and respond to saline environments that severely reduce plant growth and limit agricultural productivity. Alteration to the cell wall is one strategy that helps plants adapt to salt stress. However, we do not fully understand the physiological significance of how cell wall components respond to salt stress. Here, we show that expression of XTH30, encoding a xyloglucan endotransglucosylase/hydrolase, is strongly up-regulated in response to salt stress in Arabidopsis thaliana. Loss-of-function in XTH30 leads to increased salt tolerance and overexpression of XTH30 results in salt hypersensitivity. XTH30 is located in the plasma membrane and highly expressed in the root, flower, stem, and etiolated hypocotyl. The NaCl-induced increase in XLFG (xyloglucan-derived (XyG) oligosaccharide) of the wild type is partly blocked in xth30 mutants. Loss-of-function in XTH30 slows down the decrease of crystalline cellulose content and the depolymerization of microtubules caused by salt stress. Moreover, less Na+ accumulation in shoot and H2O2 content are found in xth30 mutants in response to salt stress. Taken together, these results indicate that XTH30 modulates XyG side chains with altered abundance of XLFG, the cellulose synthesis and cortical microtubule stability, and then negatively affects salt tolerance.},
doi = {10.1093/jxb/erz311},
journal = {Journal of Experimental Botany},
number = 19,
volume = 70,
place = {United States},
year = {2019},
month = {6}
}

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