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Title: Chlorophyll–carotenoid excitation energy transfer and charge transfer in Nannochloropsis oceanica for the regulation of photosynthesis

Abstract

Nonphotochemical quenching (NPQ) is a proxy for photoprotective thermal dissipation processes that regulate photosynthetic light harvesting. The identification of NPQ mechanisms and their molecular or physiological triggering factors under in vivo conditions is a matter of controversy. In this work, to view chlorophyll (Chl)–zeaxanthin (Zea) excitation energy transfer (EET) and charge transfer (CT) as possible NPQ mechanisms, we performed transient absorption (TA) spectroscopy on live cells of the microalga Nannochloropsis oceanica. We gained evidence for the operation of both EET and CT quenching by observing spectral features associated with the Zea S1and Zea•+excited-state absorption (ESA) signals, respectively, after Chl excitation. Knockout mutants for genes encoding either violaxanthin de-epoxidase or LHCX1 proteins exhibited strongly inhibited NPQ capabilities and lacked detectable Zea S1 and Zea•+ ESA signals in vivo, which strongly suggests that the accumulation of Zea and active LHCX1 is essential for both EET and CT quenching inN. oceanica.

Authors:
ORCiD logo [1]; ORCiD logo [1];  [1];  [1];  [1]; ORCiD logo [1]; ORCiD logo [1]; ORCiD logo [1]
  1. Univ. of California, Berkeley, CA (United States); Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
Publication Date:
Research Org.:
Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Basic Energy Sciences (BES) (SC-22). Chemical Sciences, Geosciences & Biosciences Division
OSTI Identifier:
1542372
Grant/Contract Number:  
AC02-05CH11231
Resource Type:
Accepted Manuscript
Journal Name:
Proceedings of the National Academy of Sciences of the United States of America
Additional Journal Information:
Journal Volume: 116; Journal Issue: 9; Journal ID: ISSN 0027-8424
Publisher:
National Academy of Sciences, Washington, DC (United States)
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; photosynthesis; nonphotochemical quenching; Nannochloropsis; charge transfer; excitation energy; transfer

Citation Formats

Park, Soomin, Steen, Collin J., Lyska, Dagmar, Fischer, Alexandra L., Endelman, Benjamin, Iwai, Masakazu, Niyogi, Krishna K., and Fleming, Graham R. Chlorophyll–carotenoid excitation energy transfer and charge transfer in Nannochloropsis oceanica for the regulation of photosynthesis. United States: N. p., 2019. Web. doi:10.1073/pnas.1819011116.
Park, Soomin, Steen, Collin J., Lyska, Dagmar, Fischer, Alexandra L., Endelman, Benjamin, Iwai, Masakazu, Niyogi, Krishna K., & Fleming, Graham R. Chlorophyll–carotenoid excitation energy transfer and charge transfer in Nannochloropsis oceanica for the regulation of photosynthesis. United States. doi:10.1073/pnas.1819011116.
Park, Soomin, Steen, Collin J., Lyska, Dagmar, Fischer, Alexandra L., Endelman, Benjamin, Iwai, Masakazu, Niyogi, Krishna K., and Fleming, Graham R. Mon . "Chlorophyll–carotenoid excitation energy transfer and charge transfer in Nannochloropsis oceanica for the regulation of photosynthesis". United States. doi:10.1073/pnas.1819011116. https://www.osti.gov/servlets/purl/1542372.
@article{osti_1542372,
title = {Chlorophyll–carotenoid excitation energy transfer and charge transfer in Nannochloropsis oceanica for the regulation of photosynthesis},
author = {Park, Soomin and Steen, Collin J. and Lyska, Dagmar and Fischer, Alexandra L. and Endelman, Benjamin and Iwai, Masakazu and Niyogi, Krishna K. and Fleming, Graham R.},
abstractNote = {Nonphotochemical quenching (NPQ) is a proxy for photoprotective thermal dissipation processes that regulate photosynthetic light harvesting. The identification of NPQ mechanisms and their molecular or physiological triggering factors under in vivo conditions is a matter of controversy. In this work, to view chlorophyll (Chl)–zeaxanthin (Zea) excitation energy transfer (EET) and charge transfer (CT) as possible NPQ mechanisms, we performed transient absorption (TA) spectroscopy on live cells of the microalga Nannochloropsis oceanica. We gained evidence for the operation of both EET and CT quenching by observing spectral features associated with the Zea S1and Zea•+excited-state absorption (ESA) signals, respectively, after Chl excitation. Knockout mutants for genes encoding either violaxanthin de-epoxidase or LHCX1 proteins exhibited strongly inhibited NPQ capabilities and lacked detectable Zea S1 and Zea•+ ESA signals in vivo, which strongly suggests that the accumulation of Zea and active LHCX1 is essential for both EET and CT quenching inN. oceanica.},
doi = {10.1073/pnas.1819011116},
journal = {Proceedings of the National Academy of Sciences of the United States of America},
number = 9,
volume = 116,
place = {United States},
year = {2019},
month = {2}
}

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Cited by: 15 works
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