Chlorophyll–carotenoid excitation energy transfer and charge transfer in Nannochloropsis oceanica for the regulation of photosynthesis
Abstract
Nonphotochemical quenching (NPQ) is a proxy for photoprotective thermal dissipation processes that regulate photosynthetic light harvesting. The identification of NPQ mechanisms and their molecular or physiological triggering factors under in vivo conditions is a matter of controversy. In this work, to view chlorophyll (Chl)–zeaxanthin (Zea) excitation energy transfer (EET) and charge transfer (CT) as possible NPQ mechanisms, we performed transient absorption (TA) spectroscopy on live cells of the microalga Nannochloropsis oceanica. We gained evidence for the operation of both EET and CT quenching by observing spectral features associated with the Zea S1and Zea•+excited-state absorption (ESA) signals, respectively, after Chl excitation. Knockout mutants for genes encoding either violaxanthin de-epoxidase or LHCX1 proteins exhibited strongly inhibited NPQ capabilities and lacked detectable Zea S1 and Zea•+ ESA signals in vivo, which strongly suggests that the accumulation of Zea and active LHCX1 is essential for both EET and CT quenching inN. oceanica.
- Authors:
-
[1];
[1];
[1];
[1];
[1]
- Univ. of California, Berkeley, CA (United States); Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
- Publication Date:
- Research Org.:
- Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC), Basic Energy Sciences (BES). Chemical Sciences, Geosciences, and Biosciences Division
- OSTI Identifier:
- 1542372
- Grant/Contract Number:
- AC02-05CH11231
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Proceedings of the National Academy of Sciences of the United States of America
- Additional Journal Information:
- Journal Volume: 116; Journal Issue: 9; Journal ID: ISSN 0027-8424
- Publisher:
- National Academy of Sciences, Washington, DC (United States)
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; photosynthesis; nonphotochemical quenching; Nannochloropsis; charge transfer; excitation energy; transfer
Citation Formats
Park, Soomin, Steen, Collin J., Lyska, Dagmar, Fischer, Alexandra L., Endelman, Benjamin, Iwai, Masakazu, Niyogi, Krishna K., and Fleming, Graham R. Chlorophyll–carotenoid excitation energy transfer and charge transfer in Nannochloropsis oceanica for the regulation of photosynthesis. United States: N. p., 2019.
Web. doi:10.1073/pnas.1819011116.
Park, Soomin, Steen, Collin J., Lyska, Dagmar, Fischer, Alexandra L., Endelman, Benjamin, Iwai, Masakazu, Niyogi, Krishna K., & Fleming, Graham R. Chlorophyll–carotenoid excitation energy transfer and charge transfer in Nannochloropsis oceanica for the regulation of photosynthesis. United States. https://doi.org/10.1073/pnas.1819011116
Park, Soomin, Steen, Collin J., Lyska, Dagmar, Fischer, Alexandra L., Endelman, Benjamin, Iwai, Masakazu, Niyogi, Krishna K., and Fleming, Graham R. Mon .
"Chlorophyll–carotenoid excitation energy transfer and charge transfer in Nannochloropsis oceanica for the regulation of photosynthesis". United States. https://doi.org/10.1073/pnas.1819011116. https://www.osti.gov/servlets/purl/1542372.
@article{osti_1542372,
title = {Chlorophyll–carotenoid excitation energy transfer and charge transfer in Nannochloropsis oceanica for the regulation of photosynthesis},
author = {Park, Soomin and Steen, Collin J. and Lyska, Dagmar and Fischer, Alexandra L. and Endelman, Benjamin and Iwai, Masakazu and Niyogi, Krishna K. and Fleming, Graham R.},
abstractNote = {Nonphotochemical quenching (NPQ) is a proxy for photoprotective thermal dissipation processes that regulate photosynthetic light harvesting. The identification of NPQ mechanisms and their molecular or physiological triggering factors under in vivo conditions is a matter of controversy. In this work, to view chlorophyll (Chl)–zeaxanthin (Zea) excitation energy transfer (EET) and charge transfer (CT) as possible NPQ mechanisms, we performed transient absorption (TA) spectroscopy on live cells of the microalga Nannochloropsis oceanica. We gained evidence for the operation of both EET and CT quenching by observing spectral features associated with the Zea S1and Zea•+excited-state absorption (ESA) signals, respectively, after Chl excitation. Knockout mutants for genes encoding either violaxanthin de-epoxidase or LHCX1 proteins exhibited strongly inhibited NPQ capabilities and lacked detectable Zea S1 and Zea•+ ESA signals in vivo, which strongly suggests that the accumulation of Zea and active LHCX1 is essential for both EET and CT quenching inN. oceanica.},
doi = {10.1073/pnas.1819011116},
journal = {Proceedings of the National Academy of Sciences of the United States of America},
number = 9,
volume = 116,
place = {United States},
year = {Mon Feb 11 00:00:00 EST 2019},
month = {Mon Feb 11 00:00:00 EST 2019}
}
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