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Title: Sub-micron level investigation reveals the inaccessibility of stabilized carbon in soil microaggregates

Abstract

Direct evidence-based approaches are vital to evaluating newly proposed theories on the persistence of soil organic carbon and establishing the contributions of abiotic and biotic controls. Our primary goal was to directly identify the mechanisms of organic carbon stabilization in native-state, free soil microaggregates without disrupting the aggregate microstructure using scanning transmission x-ray microscopy coupled with near edge x-ray absorption fine structure spectroscopy (STXM-NEXAFS). The influence of soil management practices on microaggregate associated-carbon was also assessed. Free, stable soil microaggregates were collected from a tropical agro-ecosystem in Cruz Alta, Brazil. The long-term experimental plots (>25 years) comparing two tillage systems: no-till and till with a complex crop rotation. Based on simultaneously collected multi-elemental associations and speciation, STXM-NEXAFS successfully provided submicron level information on organo-mineral associations. Simple organic carbon sources were found preserved within microaggregates; some still possessing original morphology, suggesting that their stabilization was not entirely governed by the substrate chemistry. Bulk analysis showed higher and younger organic carbon in microaggregates from no-till systems than tilled systems. These results provide direct submicron level evidence that the surrounding environment is involved in stabilizing organic carbon, thus favoring newly proposed concepts on the persistence of soil organic carbon.

Authors:
 [1]; ORCiD logo [1];  [1]; ORCiD logo [2];  [3];  [2];  [2]; ORCiD logo [4];  [1];  [5];  [6]
  1. Kansas State Univ., Manhattan, KS (United States). Dept. of Agronomy
  2. Canadian Light Sources, Inc., Saskatoon, SK (Canada)
  3. Kansas Dept. of Health and Environment, Topeka, KS (United States)
  4. Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States). Advanced Light Source (ALS)
  5. Federal Univ. of Santa Maria, Santa Maria, Rio Grande do Sul (Brazil)
  6. Univ. of Cruz Alta, Rio Grande do Sul (Brazil). CCGL Tec
Publication Date:
Research Org.:
Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
Sponsoring Org.:
USDOE Office of Science (SC)
OSTI Identifier:
1542344
Grant/Contract Number:  
AC02-05CH11231
Resource Type:
Accepted Manuscript
Journal Name:
Scientific Reports
Additional Journal Information:
Journal Volume: 8; Journal Issue: 1; Journal ID: ISSN 2045-2322
Publisher:
Nature Publishing Group
Country of Publication:
United States
Language:
English
Subject:
58 GEOSCIENCES; 54 ENVIRONMENTAL SCIENCES

Citation Formats

Arachchige, Pavithra S. Pitumpe, Hettiarachchi, Ganga M., Rice, Charles W., Dynes, James J., Maurmann, Leila, Wang, Jian, Karunakaran, Chithra, Kilcoyne, A. L. David, Attanayake, Chammi P., Amado, Telmo J. C., and Fiorin, Jackson E. Sub-micron level investigation reveals the inaccessibility of stabilized carbon in soil microaggregates. United States: N. p., 2018. Web. doi:10.1038/s41598-018-34981-9.
Arachchige, Pavithra S. Pitumpe, Hettiarachchi, Ganga M., Rice, Charles W., Dynes, James J., Maurmann, Leila, Wang, Jian, Karunakaran, Chithra, Kilcoyne, A. L. David, Attanayake, Chammi P., Amado, Telmo J. C., & Fiorin, Jackson E. Sub-micron level investigation reveals the inaccessibility of stabilized carbon in soil microaggregates. United States. doi:10.1038/s41598-018-34981-9.
Arachchige, Pavithra S. Pitumpe, Hettiarachchi, Ganga M., Rice, Charles W., Dynes, James J., Maurmann, Leila, Wang, Jian, Karunakaran, Chithra, Kilcoyne, A. L. David, Attanayake, Chammi P., Amado, Telmo J. C., and Fiorin, Jackson E. Wed . "Sub-micron level investigation reveals the inaccessibility of stabilized carbon in soil microaggregates". United States. doi:10.1038/s41598-018-34981-9. https://www.osti.gov/servlets/purl/1542344.
@article{osti_1542344,
title = {Sub-micron level investigation reveals the inaccessibility of stabilized carbon in soil microaggregates},
author = {Arachchige, Pavithra S. Pitumpe and Hettiarachchi, Ganga M. and Rice, Charles W. and Dynes, James J. and Maurmann, Leila and Wang, Jian and Karunakaran, Chithra and Kilcoyne, A. L. David and Attanayake, Chammi P. and Amado, Telmo J. C. and Fiorin, Jackson E.},
abstractNote = {Direct evidence-based approaches are vital to evaluating newly proposed theories on the persistence of soil organic carbon and establishing the contributions of abiotic and biotic controls. Our primary goal was to directly identify the mechanisms of organic carbon stabilization in native-state, free soil microaggregates without disrupting the aggregate microstructure using scanning transmission x-ray microscopy coupled with near edge x-ray absorption fine structure spectroscopy (STXM-NEXAFS). The influence of soil management practices on microaggregate associated-carbon was also assessed. Free, stable soil microaggregates were collected from a tropical agro-ecosystem in Cruz Alta, Brazil. The long-term experimental plots (>25 years) comparing two tillage systems: no-till and till with a complex crop rotation. Based on simultaneously collected multi-elemental associations and speciation, STXM-NEXAFS successfully provided submicron level information on organo-mineral associations. Simple organic carbon sources were found preserved within microaggregates; some still possessing original morphology, suggesting that their stabilization was not entirely governed by the substrate chemistry. Bulk analysis showed higher and younger organic carbon in microaggregates from no-till systems than tilled systems. These results provide direct submicron level evidence that the surrounding environment is involved in stabilizing organic carbon, thus favoring newly proposed concepts on the persistence of soil organic carbon.},
doi = {10.1038/s41598-018-34981-9},
journal = {Scientific Reports},
number = 1,
volume = 8,
place = {United States},
year = {2018},
month = {11}
}

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