Thermochemical wastewater valorization via enhanced microbial toxicity tolerance

Jayakody, Lahiru N.; Johnson, Christopher W.; Whitham, Jason M.; Giannone, Richard J.; Black, Brenna A.; Cleveland, Nicholas S.; Klingeman, Dawn Marie; Michener, William E.; Olstad, Jessica L.; Vardon, Derek R.; Brown, Robert C.; Brown, Steven D.; Hettich, Robert L.; Guss, Adam M.; Beckham, Gregg T.

doi:10.1039/c8ee00460a

Title: Thermochemical wastewater valorization via enhanced microbial toxicity tolerance

Journal Article · 17 April 2018 · Energy & Environmental Science

DOI: https://doi.org/10.1039/c8ee00460a · OSTI ID:1542241

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^[2]; Black, Brenna A. ^[1]; Cleveland, Nicholas S. ^[1];

^[2]; Michener, William E. ^[1]; Olstad, Jessica L. ^[1];

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National Renewable Energy Lab. (NREL), Golden, CO (United States)
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
Iowa State Univ., Ames, IA (United States)
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); LanzaTech, Inc., Skokie, IL (United States)

Thermochemical (TC) biomass conversion processes such as pyrolysis and liquefaction generate considerable amounts of wastewater, which often contains highly toxic compounds that are incredibly challenging to convert via standard wastewater treatment approaches such as anaerobic digestion. These streams represent a cost for TC biorefineries, and a potential valorization opportunity, if effective conversion methods are developed. The primary challenge hindering microbial conversion of TC wastewater is toxicity. In this study, we employ a robust bacterium, Pseudomonas putida, with TC wastewater streams to demonstrate that aldehydes are the most inhibitory compounds in these streams. Proteomics, transcriptomics, and fluorescence-based immunoassays of P. putida grown in a representative wastewater stream indicate that stress results from protein damage, which we hypothesize is a primary toxicity mechanism. Constitutive overexpression of the chaperone genes, groEL, groES, and clpB, in a genome-reduced P. putida strain improves the tolerance towards multiple TC wastewater samples up to 200-fold. Moreover, the concentration ranges of TC wastewater are industrially relevant for further bioprocess development for all wastewater streams examined here, representing different TC process configurations. Furthermore, we demonstrate proof-of-concept polyhydroxyalkanoate production from the usable carbon in an exemplary TC wastewater stream. Altogether, this study demonstrates that protein quality control machinery and repair mechanisms can enable substantial gains in microbial tolerance to highly toxic substrates, including heterogeneous waste streams. When coupled to other metabolic engineering advances such as expanded substrate utilization and enhanced product accumulation, this study generally enables new strategies for biological conversion of highly-toxic, organic-rich wastewater via engineered aerobic monocultures or designer consortia.

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Research Organization:: Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER)

Grant/Contract Number:: AC05-00OR22725; AC36-08GO28308; AC02-05CH11231

OSTI ID:: 1542241

Alternate ID(s):: OSTI ID: 1434804

Report Number(s):: NREL/JA-2A00-71485; EESNBY

Journal Information:: Energy & Environmental Science, Vol. 11, Issue 6; ISSN 1754-5692

Publisher:: Royal Society of ChemistryCopyright Statement

Country of Publication:: United States

Language:: English

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Cited by: 65 works

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