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Title: High marker density GWAS provides novel insights into the genomic architecture of terpene oil yield in Eucalyptus

Abstract

Terpenoid-based essential oils are economically important commodities, yet beyond their biosynthetic pathways, little is known about the genetic architecture of terpene oil yield from plants. Transport, storage, evaporative loss, transcriptional regulation and precursor competition may be important contributors to this complex trait. Here, we associate 2.39 million single nucleotide polymorphisms derived from shallow whole-genome sequencing of 468 Eucalyptus polybractea individuals with 12 traits related to the overall terpene yield, eight direct measures of terpene concentration and four biomass-related traits. Our results show that in addition to terpene biosynthesis, development of secretory cavities, where terpenes are both synthesized and stored, and transport of terpenes were important components of terpene yield. For sesquiterpene concentrations, the availability of precursors in the cytosol was important. Candidate terpene synthase genes for the production of 1,8-cineole and α-pinene, and β-pinene (which comprised > 80% of the total terpenes) were functionally characterized as a 1,8-cineole synthase and a β/α-pinene synthase. Our results provide novel insights into the genomic architecture of terpene yield and we provide candidate genes for breeding or engineering of crops for biofuels or the production of industrially valuable terpenes.

Authors:
 [1];  [2];  [3];  [4];  [5];  [5];  [5]
  1. Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); The Australian National Univ., Canberra, ACT (Australia)
  2. The Australian National Univ., Canberra, ACT (Australia); Michigan Technological Univ., Houghton, MI (United States)
  3. The Australian National Univ., Canberra, ACT (Australia)
  4. The Australian National Univ., Canberra, ACT (Australia); CSIRO, Canberra, ACT (Australia)
  5. Martin-Luther Univ. Halle-Wittenberg, Halle (Saale) (Germany)
Publication Date:
Research Org.:
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1542234
Alternate Identifier(s):
OSTI ID: 1560214
Grant/Contract Number:  
AC05-00OR22725
Resource Type:
Accepted Manuscript
Journal Name:
New Phytologist
Additional Journal Information:
Journal Volume: 223; Journal Issue: 3; Journal ID: ISSN 0028-646X
Publisher:
Wiley
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; 1,8‐cineole; essential oil; eucalypt; genome‐wide association study (GWAS); pinene; terpene oil yield; terpene synthase; terpenes

Citation Formats

Kainer, David, Külheim, Carsten, Foley, William J., Padovan, Amanda, Degenhardt, Joerg, Krause, Sandra, and Mondal, Prodyut. High marker density GWAS provides novel insights into the genomic architecture of terpene oil yield in Eucalyptus. United States: N. p., 2019. Web. doi:10.1111/nph.15887.
Kainer, David, Külheim, Carsten, Foley, William J., Padovan, Amanda, Degenhardt, Joerg, Krause, Sandra, & Mondal, Prodyut. High marker density GWAS provides novel insights into the genomic architecture of terpene oil yield in Eucalyptus. United States. doi:10.1111/nph.15887.
Kainer, David, Külheim, Carsten, Foley, William J., Padovan, Amanda, Degenhardt, Joerg, Krause, Sandra, and Mondal, Prodyut. Tue . "High marker density GWAS provides novel insights into the genomic architecture of terpene oil yield in Eucalyptus". United States. doi:10.1111/nph.15887.
@article{osti_1542234,
title = {High marker density GWAS provides novel insights into the genomic architecture of terpene oil yield in Eucalyptus},
author = {Kainer, David and Külheim, Carsten and Foley, William J. and Padovan, Amanda and Degenhardt, Joerg and Krause, Sandra and Mondal, Prodyut},
abstractNote = {Terpenoid-based essential oils are economically important commodities, yet beyond their biosynthetic pathways, little is known about the genetic architecture of terpene oil yield from plants. Transport, storage, evaporative loss, transcriptional regulation and precursor competition may be important contributors to this complex trait. Here, we associate 2.39 million single nucleotide polymorphisms derived from shallow whole-genome sequencing of 468 Eucalyptus polybractea individuals with 12 traits related to the overall terpene yield, eight direct measures of terpene concentration and four biomass-related traits. Our results show that in addition to terpene biosynthesis, development of secretory cavities, where terpenes are both synthesized and stored, and transport of terpenes were important components of terpene yield. For sesquiterpene concentrations, the availability of precursors in the cytosol was important. Candidate terpene synthase genes for the production of 1,8-cineole and α-pinene, and β-pinene (which comprised > 80% of the total terpenes) were functionally characterized as a 1,8-cineole synthase and a β/α-pinene synthase. Our results provide novel insights into the genomic architecture of terpene yield and we provide candidate genes for breeding or engineering of crops for biofuels or the production of industrially valuable terpenes.},
doi = {10.1111/nph.15887},
journal = {New Phytologist},
number = 3,
volume = 223,
place = {United States},
year = {2019},
month = {5}
}

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