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Title: PtWOX11 acts as master regulator conducting the expression of key transcription factors to induce de novo shoot organogenesis in poplar

Journal Article · · Plant Molecular Biology
ORCiD logo [1]; ORCiD logo [2];  [1];  [3];  [3];  [1];  [1];  [1];  [1];  [4]; ORCiD logo [5];  [6];  [7]
  1. Fujian Agriculture and Forestry Univ., Fuzhou (China)
  2. Chinese Academy of Forestry, Beijing (China); Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
  3. RIKEN Center for Sustainable Resource Science, Yokohama (Japan)
  4. Indian Inst. of Science Education and Research, Thiruvananthapuram (India)
  5. Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
  6. Chinese Academy of Forestry, Beijing (China)
  7. Fujian Agriculture and Forestry Univ., Fuzhou (China); RIKEN Center for Sustainable Resource Science, Yokohama (Japan)

De novo shoot regeneration is a prerequisite for propagation and genetic engineering of elite cultivars in forestry. Yet, the regulatory mechanism of de novo organogenesis is poorly understood in tree species. We previously showed that WUSCHEL (WUS)-RELATED HOMEOBOX 11 (PtWOX11) of the hybrid poplar clone 84K (Populus alba × P. glandulosa) promotes de novo root formation. Here, we found that PtWOX11 also regulates de novo shoot regeneration in poplar. The overexpression of PtWOX11 enhanced de novo shoot formation, whereas overexpression of PtWOX11 fused with the transcriptional repressor domain (PtWOX11-SRDX) or reduced expression of PtWOX11 inhibited this process, indicating that PtWOX11 promotes de novo shoot organogenesis. Although PtWOX11 promotes callus formation, overexpression of PtWOX11 and PtWOX11-SRDX also produced increased and decreased numbers of de novo shoots per unit weight, respectively, implying that PtWOX11 promotes de novo shoot organogenesis partially by regulating the intrinsic mechanism of shoot development. RNA-seq and qPCR analysis further revealed that PtWOX11 activates the expression of PLETHORA1 (PtPLT1) and PtPLT2, whose Arabidopsis paralogs establish the acquisition of pluripotency, during incubation on callus-inducing medium. Moreover, PtWOX11 activates the expression of shoot-promoting factors and meristem regulators such as CUP-SHAPED COTYLEDON2 (PtCUC2), PtCUC3, WUS and SHOOT MERISTEMLESS to fulfill shoot regeneration during incubation on shoot-inducing medium. These results imply that PtWOX11 acts as a central regulator of the expression of key genes to cause de novo shoot formation. Our studies further provide a possible means to genetically engineer economically important tree species for their micropropagation.

Research Organization:
Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)
Sponsoring Organization:
USDOE Office of Science (SC), Biological and Environmental Research (BER); National Natural Science Foundation of China (NSFC); National Key Research and Development Program of China; Natural Science Foundation of Fujian province
Grant/Contract Number:
AC05-00OR22725
OSTI ID:
1542226
Journal Information:
Plant Molecular Biology, Vol. 98, Issue 4-5; ISSN 0167-4412
Publisher:
SpringerCopyright Statement
Country of Publication:
United States
Language:
English
Citation Metrics:
Cited by: 10 works
Citation information provided by
Web of Science

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Cited By (1)


Figures / Tables (8)