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Title: High-viscosity injector-based pink-beam serial crystallography of microcrystals at a synchrotron radiation source

Abstract

Since the first successful serial crystallography (SX) experiment at a synchrotron radiation source, the popularity of this approach has continued to grow showing that third-generation synchrotrons can be viable alternatives to scarce X-ray free-electron laser sources. Synchrotron radiation flux may be increased ∼100 times by a moderate increase in the bandwidth (`pink beam' conditions) at some cost to data analysis complexity. Here, we report the first high-viscosity injector-based pink-beam SX experiments. The structures of proteinase K (PK) and A 2Aadenosine receptor (A 2AAR) were determined to resolutions of 1.8 and 4.2 Å using 4 and 24 consecutive 100 ps X-ray pulse exposures, respectively. Strong PK data were processed using existing Laue approaches, while weaker A 2AAR data required an alternative data-processing strategy. This demonstration of the feasibility presents new opportunities for time-resolved experiments with microcrystals to study structural changes in real time at pink-beam synchrotron beamlines worldwide.

Authors:
ORCiD logo [1];  [1];  [1];  [1];  [1]; ORCiD logo [1];  [1];  [1];  [2];  [2];  [3]; ORCiD logo [4];  [1];  [1];  [1]; ORCiD logo [1];  [1]; ORCiD logo [1]; ORCiD logo [2];  [4] more »;  [1] « less
  1. Arizona State Univ., Tempe, AZ (United States)
  2. Argonne National Lab. (ANL), Lemont, IL (United States)
  3. The Univ. of Chicago, Argonne National Lab., Lemont, IL (United States)
  4. Univ. of Southern California, Los Angeles, CA (United States)
Publication Date:
Research Org.:
Argonne National Lab. (ANL), Argonne, IL (United States)
Sponsoring Org.:
National Institutes of Health (NIH), National Institute of General Medical Sciences; National Institutes of Health (NIH), National Cancer Institute; Mayo Clinic; Arizona State University; National Science Foundation (NSF), BioXFEL; National Science Foundation (NSF); USDOE
OSTI Identifier:
1542189
Grant/Contract Number:  
AC02-06CH11357
Resource Type:
Accepted Manuscript
Journal Name:
IUCrJ
Additional Journal Information:
Journal Volume: 6; Journal Issue: 3; Journal ID: ISSN 2052-2525
Publisher:
International Union of Crystallography
Country of Publication:
United States
Language:
English
Subject:
lipidic cubic phase; pink beam; protein dynamics; serial crystallography; time-resolved; third-generation synchrotrons; injector-based serial crystallography; structure determination; membrane proteins; protein structures; X-ray crystallography; structural biology

Citation Formats

Martin-Garcia, Jose M., Zhu, Lan, Mendez, Derek, Lee, Ming-Yue, Chun, Eugene, Li, Chufeng, Hu, Hao, Subramanian, Ganesh, Kissick, David, Ogata, Craig, Henning, Robert, Ishchenko, Andrii, Dobson, Zachary, Zhang, Shangji, Weierstall, Uwe, Spence, John C. H., Fromme, Petra, Zatsepin, Nadia A., Fischetti, Robert F., Cherezov, Vadim, and Liu, Wei. High-viscosity injector-based pink-beam serial crystallography of microcrystals at a synchrotron radiation source. United States: N. p., 2019. Web. doi:10.1107/S205225251900263X.
Martin-Garcia, Jose M., Zhu, Lan, Mendez, Derek, Lee, Ming-Yue, Chun, Eugene, Li, Chufeng, Hu, Hao, Subramanian, Ganesh, Kissick, David, Ogata, Craig, Henning, Robert, Ishchenko, Andrii, Dobson, Zachary, Zhang, Shangji, Weierstall, Uwe, Spence, John C. H., Fromme, Petra, Zatsepin, Nadia A., Fischetti, Robert F., Cherezov, Vadim, & Liu, Wei. High-viscosity injector-based pink-beam serial crystallography of microcrystals at a synchrotron radiation source. United States. doi:10.1107/S205225251900263X.
Martin-Garcia, Jose M., Zhu, Lan, Mendez, Derek, Lee, Ming-Yue, Chun, Eugene, Li, Chufeng, Hu, Hao, Subramanian, Ganesh, Kissick, David, Ogata, Craig, Henning, Robert, Ishchenko, Andrii, Dobson, Zachary, Zhang, Shangji, Weierstall, Uwe, Spence, John C. H., Fromme, Petra, Zatsepin, Nadia A., Fischetti, Robert F., Cherezov, Vadim, and Liu, Wei. Fri . "High-viscosity injector-based pink-beam serial crystallography of microcrystals at a synchrotron radiation source". United States. doi:10.1107/S205225251900263X. https://www.osti.gov/servlets/purl/1542189.
@article{osti_1542189,
title = {High-viscosity injector-based pink-beam serial crystallography of microcrystals at a synchrotron radiation source},
author = {Martin-Garcia, Jose M. and Zhu, Lan and Mendez, Derek and Lee, Ming-Yue and Chun, Eugene and Li, Chufeng and Hu, Hao and Subramanian, Ganesh and Kissick, David and Ogata, Craig and Henning, Robert and Ishchenko, Andrii and Dobson, Zachary and Zhang, Shangji and Weierstall, Uwe and Spence, John C. H. and Fromme, Petra and Zatsepin, Nadia A. and Fischetti, Robert F. and Cherezov, Vadim and Liu, Wei},
abstractNote = {Since the first successful serial crystallography (SX) experiment at a synchrotron radiation source, the popularity of this approach has continued to grow showing that third-generation synchrotrons can be viable alternatives to scarce X-ray free-electron laser sources. Synchrotron radiation flux may be increased ∼100 times by a moderate increase in the bandwidth (`pink beam' conditions) at some cost to data analysis complexity. Here, we report the first high-viscosity injector-based pink-beam SX experiments. The structures of proteinase K (PK) and A2Aadenosine receptor (A2AAR) were determined to resolutions of 1.8 and 4.2 Å using 4 and 24 consecutive 100 ps X-ray pulse exposures, respectively. Strong PK data were processed using existing Laue approaches, while weaker A2AAR data required an alternative data-processing strategy. This demonstration of the feasibility presents new opportunities for time-resolved experiments with microcrystals to study structural changes in real time at pink-beam synchrotron beamlines worldwide.},
doi = {10.1107/S205225251900263X},
journal = {IUCrJ},
number = 3,
volume = 6,
place = {United States},
year = {2019},
month = {4}
}

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