Correlating structural and photochemical heterogeneity in cyanobacteriochrome NpR6012g4

Lim, Sunghyuk; Yu, Qinhong; Gottlieb, Sean M.; Chang, Che-Wei; Rockwell, Nathan C.; Martin, Shelley S.; Madsen, Dorte; Lagarias, J. Clark; Larsen, Delmar S.; Ames, James B.

doi:10.1073/pnas.1720682115

Title: Correlating structural and photochemical heterogeneity in cyanobacteriochrome NpR6012g4

Journal Article · 09 April 2018 · Proceedings of the National Academy of Sciences of the United States of America

DOI: https://doi.org/10.1073/pnas.1720682115 · OSTI ID:1540279

Lim, Sunghyuk ^[1]; Yu, Qinhong ^[1]; Gottlieb, Sean M. ^[2]; Chang, Che-Wei ^[1]; Rockwell, Nathan C. ^[1]; Martin, Shelley S. ^[1]; Madsen, Dorte ^[1];

^[1]; Larsen, Delmar S. ^[1]; Ames, James B. ^[1]

Univ. of California, Davis, CA (United States)
Univ. of California, Davis, CA (United States); Allan Hancock College, Santa Maria, CA (United States)

Phytochrome photoreceptors control plant growth, development, and the shade avoidance response that limits crop yield in high-density agricultural plantings. Cyanobacteriochromes (CBCRs) are distantly related photosensory proteins that control cyanobacterial metabolism and behavior in response to light. Photoreceptors in both families reversibly photoconvert between two photostates via photoisomerization of linear tetrapyrrole (bilin) chromophores. Spectroscopic and biochemical reprorts have demonstrated heterogeneity in both photostates, but the structural basis for such heterogeneity remains unclear. We discuss solution NMR structures for both photostates of the red/green CBCR NpR6012g4 fromNostoc punctiforme. In addition to identifying structural changes accompanying photoconversion, these structures reveal structural heterogeneity for residues Trp655 and Asp657 in the red-absorbing NpR6012g4 dark state, yielding two distinct environments for the phycocyanobilin chromophore. We use site-directed mutagenesis and fluorescence and absorbance spectroscopy to assign an orange-absorbing population in the NpR6012g4 dark state to the minority configuration for Asp657. This population does not undergo full, productive photoconversion, as shown by time-resolved spectroscopy and absorption spectroscopy at cryogenic temperature. Our research thus elucidate the spectral and photochemical consequences of structural heterogeneity in a member of the phytochrome superfamily, insights that should inform efforts to improve photochemical or fluorescence quantum yields in the phytochrome superfamily.

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Research Organization:: Univ. of California, Davis, CA (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Basic Energy Sciences (BES). Chemical Sciences, Geosciences, and Biosciences Division; National Institutes of Health (NIH)

Grant/Contract Number:: SC0002395; FG02-09ER16117

OSTI ID:: 1540279

Journal Information:: Proceedings of the National Academy of Sciences of the United States of America, Vol. 115, Issue 17; ISSN 0027-8424

Publisher:: National Academy of Sciences, Washington, DC (United States)Copyright Statement

Country of Publication:: United States

Language:: English

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