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Title: Correlating structural and photochemical heterogeneity in cyanobacteriochrome NpR6012g4

Journal Article · · Proceedings of the National Academy of Sciences of the United States of America

Phytochrome photoreceptors control plant growth, development, and the shade avoidance response that limits crop yield in high-density agricultural plantings. Cyanobacteriochromes (CBCRs) are distantly related photosensory proteins that control cyanobacterial metabolism and behavior in response to light. Photoreceptors in both families reversibly photoconvert between two photostates via photoisomerization of linear tetrapyrrole (bilin) chromophores. Spectroscopic and biochemical reprorts have demonstrated heterogeneity in both photostates, but the structural basis for such heterogeneity remains unclear. We discuss solution NMR structures for both photostates of the red/green CBCR NpR6012g4 fromNostoc punctiforme. In addition to identifying structural changes accompanying photoconversion, these structures reveal structural heterogeneity for residues Trp655 and Asp657 in the red-absorbing NpR6012g4 dark state, yielding two distinct environments for the phycocyanobilin chromophore. We use site-directed mutagenesis and fluorescence and absorbance spectroscopy to assign an orange-absorbing population in the NpR6012g4 dark state to the minority configuration for Asp657. This population does not undergo full, productive photoconversion, as shown by time-resolved spectroscopy and absorption spectroscopy at cryogenic temperature. Our research thus elucidate the spectral and photochemical consequences of structural heterogeneity in a member of the phytochrome superfamily, insights that should inform efforts to improve photochemical or fluorescence quantum yields in the phytochrome superfamily.

Research Organization:
Univ. of California, Davis, CA (United States)
Sponsoring Organization:
USDOE Office of Science (SC), Basic Energy Sciences (BES). Chemical Sciences, Geosciences, and Biosciences Division; National Institutes of Health (NIH)
Grant/Contract Number:
SC0002395; FG02-09ER16117
OSTI ID:
1540279
Journal Information:
Proceedings of the National Academy of Sciences of the United States of America, Vol. 115, Issue 17; ISSN 0027-8424
Publisher:
National Academy of Sciences, Washington, DC (United States)Copyright Statement
Country of Publication:
United States
Language:
English

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Structural elements regulating the photochromicity in a cyanobacteriochrome text January 2020
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