Putidaredoxin Binds to the Same Site on Cytochrome P450cam in the Open and Closed Conformation
Abstract
Cytochrome P450 CYP101A1 (P450cam) hydroxylates camphor by receiving two distinct electrons from its unique reductase, putidaredoxin (Pdx). Upon binding ferric P450cam, Pdx is now known to trigger a conformational change in the enzyme. This Pdx-induced conversion may provide the trigger to coordinate enzyme turnover and protect the enzyme from oxidative damage, so the interactions responsible for this conversion are of significant interest at present. This proposed role for Pdx requires that its interactions with P450cam be different for the open and closed conformations. Here, we show that the binding thermodynamics of Pdx does indeed differ in the predicted way when the conformation of P450cam is held in different states. However, double electron–electron resonance measurements of intermolecular distances in the Pdx/P450cam complex show that the geometry of the complex is nearly identical for the open and closed states of P450cam. These studies show that Pdx appears to make a single distinct interaction with its binding site on the enzyme and triggers the conformational change through very subtle structural interactions.
- Authors:
-
- Univ. of California, Davis, CA (United States); Max-Planck-Inst. for Biophysical Chemistry, Göttingen (Germany)
- Univ. of Oxford (United Kingdom)
- Univ. of California, Davis, CA (United States)
- Publication Date:
- Research Org.:
- Univ. of California, Davis, CA (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC); National Institutes of Health (NIH); Engineering and Physical Sciences Research Council (EPSRC)
- OSTI Identifier:
- 1534424
- Grant/Contract Number:
- SC0002395; GM41049; FG02-09ER16117; EP/L011972/1
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Biochemistry
- Additional Journal Information:
- Journal Volume: 56; Journal Issue: 33; Journal ID: ISSN 0006-2960
- Publisher:
- American Chemical Society (ACS)
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY; 59 BASIC BIOLOGICAL SCIENCES; Biochemistry & molecular biology; Interfaces; Peptides and proteins; Ligands; Crystal structure; Conformation
Citation Formats
Liou, Shu-Hao, Myers, William K., Oswald, Jason D., Britt, R. David, and Goodin, David B. Putidaredoxin Binds to the Same Site on Cytochrome P450cam in the Open and Closed Conformation. United States: N. p., 2017.
Web. doi:10.1021/acs.biochem.7b00564.
Liou, Shu-Hao, Myers, William K., Oswald, Jason D., Britt, R. David, & Goodin, David B. Putidaredoxin Binds to the Same Site on Cytochrome P450cam in the Open and Closed Conformation. United States. https://doi.org/10.1021/acs.biochem.7b00564
Liou, Shu-Hao, Myers, William K., Oswald, Jason D., Britt, R. David, and Goodin, David B. Tue .
"Putidaredoxin Binds to the Same Site on Cytochrome P450cam in the Open and Closed Conformation". United States. https://doi.org/10.1021/acs.biochem.7b00564. https://www.osti.gov/servlets/purl/1534424.
@article{osti_1534424,
title = {Putidaredoxin Binds to the Same Site on Cytochrome P450cam in the Open and Closed Conformation},
author = {Liou, Shu-Hao and Myers, William K. and Oswald, Jason D. and Britt, R. David and Goodin, David B.},
abstractNote = {Cytochrome P450 CYP101A1 (P450cam) hydroxylates camphor by receiving two distinct electrons from its unique reductase, putidaredoxin (Pdx). Upon binding ferric P450cam, Pdx is now known to trigger a conformational change in the enzyme. This Pdx-induced conversion may provide the trigger to coordinate enzyme turnover and protect the enzyme from oxidative damage, so the interactions responsible for this conversion are of significant interest at present. This proposed role for Pdx requires that its interactions with P450cam be different for the open and closed conformations. Here, we show that the binding thermodynamics of Pdx does indeed differ in the predicted way when the conformation of P450cam is held in different states. However, double electron–electron resonance measurements of intermolecular distances in the Pdx/P450cam complex show that the geometry of the complex is nearly identical for the open and closed states of P450cam. These studies show that Pdx appears to make a single distinct interaction with its binding site on the enzyme and triggers the conformational change through very subtle structural interactions.},
doi = {10.1021/acs.biochem.7b00564},
journal = {Biochemistry},
number = 33,
volume = 56,
place = {United States},
year = {2017},
month = {7}
}
Web of Science
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