Coherent diffractive imaging of microtubules using an X-ray laser

Brändén, Gisela; Hammarin, Greger; Harimoorthy, Rajiv; Johansson, Alexander; Arnlund, David; Malmerberg, Erik; Barty, Anton; Tångefjord, Stefan; Berntsen, Peter; DePonte, Daniel P.; Seuring, Carolin; White, Thomas A.; Stellato, Francesco; Bean, Richard; Beyerlein, Kenneth R.; Chavas, Leonard M. G.; Fleckenstein, Holger; Gati, Cornelius; Ghoshdastider, Umesh; Gumprecht, Lars; Oberthür, Dominik; Popp, David; Seibert, Marvin; Tilp, Thomas; Messerschmidt, Marc; Williams, Garth J.; Loh, N. Duane; Chapman, Henry N.; Zwart, Peter; Liang, Mengning; Boutet, Sébastien; Robinson, Robert C.; Neutze, Richard

doi:10.1038/s41467-019-10448-x

Title: Coherent diffractive imaging of microtubules using an X-ray laser

Journal Article · 13 June 2019 · Nature Communications

DOI: https://doi.org/10.1038/s41467-019-10448-x · OSTI ID:1532435

Brändén, Gisela ^[1];

^[1]; Harimoorthy, Rajiv ^[1]; Johansson, Alexander ^[1]; Arnlund, David ^[1]; Malmerberg, Erik ^[2]; Barty, Anton ^[3]; Tångefjord, Stefan ^[4]; Berntsen, Peter ^[1]; DePonte, Daniel P. ^[5];

^[6]; White, Thomas A. ^[3]; Stellato, Francesco ^[3]; Bean, Richard ^[3];

^[3]; Chavas, Leonard M. G. ^[3]; Fleckenstein, Holger ^[3]; Gati, Cornelius ^[3]; Ghoshdastider, Umesh ^[7]; Gumprecht, Lars ^[3] more »

Univ. of Gothenburg, Gothenburg (Sweden)
Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
Deutsches Elektronen-Synchrotron (DESY), Hamburg (Germany)
AstraZeneca R&D, Molndal (Sweden)
SLAC National Accelerator Lab., Menlo Park, CA (United States)
Deutsches Elektronen-Synchrotron (DESY), Hamburg (Germany); The Hamburg Center for Ultrafast Imaging, Hamburg (Germany)
A*STAR (Agency for Science, Technology and Research) (Singapore)
National Univ. of Singapore (Singapore)
Deutsches Elektronen-Synchrotron (DESY), Hamburg (Germany); The Hamburg Center for Ultrafast Imaging, Hamburg (Germany); Univ. of Hamburg, Hamburg (Germany)
Deutsches Elektronen-Synchrotron (DESY), Hamburg (Germany); SLAC National Accelerator Lab., Menlo Park, CA (United States)
A*STAR (Agency for Science, Technology and Research) (Singapore); National Univ. of Singapore (Singapore); Okayama Univ., Okayama (Japan)

X-ray free electron lasers (XFELs) create new possibilities for structural studies of biological objects that extend beyond what is possible with synchrotron radiation. Serial femtosecond crystallography has allowed high-resolution structures to be determined from micro-meter sized crystals, whereas single particle coherent X-ray imaging requires development to extend the resolution beyond a few tens of nanometers. Here we describe an intermediate approach: the XFEL imaging of biological assemblies with helical symmetry. We collected X-ray scattering images from samples of microtubules injected across an XFEL beam using a liquid microjet, sorted these images into class averages, merged these data into a diffraction pattern extending to 2 nm resolution, and reconstructed these data into a projection image of the microtubule. Details such as the 4 nm tubulin monomer became visible in this reconstruction. These results illustrate the potential of single-molecule X-ray imaging of biological assembles with helical symmetry at room temperature.

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Research Organization:: SLAC National Accelerator Laboratory (SLAC), Menlo Park, CA (United States)

Sponsoring Organization:: USDOE Office of Science (SC)

Grant/Contract Number:: AC02-76SF00515

OSTI ID:: 1532435

Journal Information:: Nature Communications, Journal Name: Nature Communications Journal Issue: 1 Vol. 10; ISSN 2041-1723

Publisher:: Nature Publishing GroupCopyright Statement

Country of Publication:: United States

Language:: English

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