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Title: Coherent diffractive imaging of microtubules using an X-ray laser

Authors:
 [1]; ORCiD logo [1];  [1];  [1];  [1];  [2];  [3];  [4];  [1];  [5]; ORCiD logo [6];  [3];  [3];  [3]; ORCiD logo [3];  [3];  [3];  [3];  [7];  [3] more »;  [3];  [7];  [5];  [3];  [5];  [5];  [8]; ORCiD logo [9];  [2];  [10];  [5];  [11]; ORCiD logo [1] « less
  1. Univ. of Gothenburg, Gothenburg (Sweden)
  2. Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
  3. Deutsches Elektronen-Synchrotron (DESY), Hamburg (Germany)
  4. AstraZeneca R&D, Molndal (Sweden)
  5. SLAC National Accelerator Lab., Menlo Park, CA (United States)
  6. Deutsches Elektronen-Synchrotron (DESY), Hamburg (Germany); The Hamburg Center for Ultrafast Imaging, Hamburg (Germany)
  7. A*STAR (Agency for Science, Technology and Research) (Singapore)
  8. National Univ. of Singapore (Singapore)
  9. Deutsches Elektronen-Synchrotron (DESY), Hamburg (Germany); The Hamburg Center for Ultrafast Imaging, Hamburg (Germany); Univ. of Hamburg, Hamburg (Germany)
  10. Deutsches Elektronen-Synchrotron (DESY), Hamburg (Germany); SLAC National Accelerator Lab., Menlo Park, CA (United States)
  11. A*STAR (Agency for Science, Technology and Research) (Singapore); National Univ. of Singapore (Singapore); Okayama Univ., Okayama (Japan)
Publication Date:
Research Org.:
SLAC National Accelerator Lab., Menlo Park, CA (United States); Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
Sponsoring Org.:
USDOE Office of Science (SC)
OSTI Identifier:
1532435
Alternate Identifier(s):
OSTI ID: 1559264
Grant/Contract Number:  
AC02-76SF00515; 2015-00560; KAW 2012.0284; KAW 2012.0275; KAW 2014.0275; AC02-05CH11231
Resource Type:
Accepted Manuscript
Journal Name:
Nature Communications
Additional Journal Information:
Journal Volume: 10; Journal Issue: 1; Journal ID: ISSN 2041-1723
Publisher:
Nature Publishing Group
Country of Publication:
United States
Language:
English
Subject:
47 OTHER INSTRUMENTATION

Citation Formats

Brändén, Gisela, Hammarin, Greger, Harimoorthy, Rajiv, Johansson, Alexander, Arnlund, David, Malmerberg, Erik, Barty, Anton, Tångefjord, Stefan, Berntsen, Peter, DePonte, Daniel P., Seuring, Carolin, White, Thomas A., Stellato, Francesco, Bean, Richard, Beyerlein, Kenneth R., Chavas, Leonard M. G., Fleckenstein, Holger, Gati, Cornelius, Ghoshdastider, Umesh, Gumprecht, Lars, Oberthür, Dominik, Popp, David, Seibert, Marvin, Tilp, Thomas, Messerschmidt, Marc, Williams, Garth J., Loh, N. Duane, Chapman, Henry N., Zwart, Peter, Liang, Mengning, Boutet, Sébastien, Robinson, Robert C., and Neutze, Richard. Coherent diffractive imaging of microtubules using an X-ray laser. United States: N. p., 2019. Web. doi:10.1038/s41467-019-10448-x.
Brändén, Gisela, Hammarin, Greger, Harimoorthy, Rajiv, Johansson, Alexander, Arnlund, David, Malmerberg, Erik, Barty, Anton, Tångefjord, Stefan, Berntsen, Peter, DePonte, Daniel P., Seuring, Carolin, White, Thomas A., Stellato, Francesco, Bean, Richard, Beyerlein, Kenneth R., Chavas, Leonard M. G., Fleckenstein, Holger, Gati, Cornelius, Ghoshdastider, Umesh, Gumprecht, Lars, Oberthür, Dominik, Popp, David, Seibert, Marvin, Tilp, Thomas, Messerschmidt, Marc, Williams, Garth J., Loh, N. Duane, Chapman, Henry N., Zwart, Peter, Liang, Mengning, Boutet, Sébastien, Robinson, Robert C., & Neutze, Richard. Coherent diffractive imaging of microtubules using an X-ray laser. United States. doi:10.1038/s41467-019-10448-x.
Brändén, Gisela, Hammarin, Greger, Harimoorthy, Rajiv, Johansson, Alexander, Arnlund, David, Malmerberg, Erik, Barty, Anton, Tångefjord, Stefan, Berntsen, Peter, DePonte, Daniel P., Seuring, Carolin, White, Thomas A., Stellato, Francesco, Bean, Richard, Beyerlein, Kenneth R., Chavas, Leonard M. G., Fleckenstein, Holger, Gati, Cornelius, Ghoshdastider, Umesh, Gumprecht, Lars, Oberthür, Dominik, Popp, David, Seibert, Marvin, Tilp, Thomas, Messerschmidt, Marc, Williams, Garth J., Loh, N. Duane, Chapman, Henry N., Zwart, Peter, Liang, Mengning, Boutet, Sébastien, Robinson, Robert C., and Neutze, Richard. Thu . "Coherent diffractive imaging of microtubules using an X-ray laser". United States. doi:10.1038/s41467-019-10448-x. https://www.osti.gov/servlets/purl/1532435.
@article{osti_1532435,
title = {Coherent diffractive imaging of microtubules using an X-ray laser},
author = {Brändén, Gisela and Hammarin, Greger and Harimoorthy, Rajiv and Johansson, Alexander and Arnlund, David and Malmerberg, Erik and Barty, Anton and Tångefjord, Stefan and Berntsen, Peter and DePonte, Daniel P. and Seuring, Carolin and White, Thomas A. and Stellato, Francesco and Bean, Richard and Beyerlein, Kenneth R. and Chavas, Leonard M. G. and Fleckenstein, Holger and Gati, Cornelius and Ghoshdastider, Umesh and Gumprecht, Lars and Oberthür, Dominik and Popp, David and Seibert, Marvin and Tilp, Thomas and Messerschmidt, Marc and Williams, Garth J. and Loh, N. Duane and Chapman, Henry N. and Zwart, Peter and Liang, Mengning and Boutet, Sébastien and Robinson, Robert C. and Neutze, Richard},
abstractNote = {},
doi = {10.1038/s41467-019-10448-x},
journal = {Nature Communications},
number = 1,
volume = 10,
place = {United States},
year = {2019},
month = {6}
}

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Cited by: 3 works
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Figures / Tables:

Figure 1 Figure 1: Schematic illustration of the experimental setup. Samples of preformed microtubules were injected across a focused X-ray free electron laser (XFEL) beam. Diffraction data were recorded on a CSPAD X-ray detector which was read out at 120 Hz, which matched the incoming repetition rate of the XFEL. Under themore » experimental conditions approximately twenty microtubules were intercepted by the X-ray beam as it passed through the microjet. Figures 1, 2, and 5 were originally presented in the doctoral thesis of Harimoorthy59« less

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    Works referencing / citing this record:

    Flow-aligned, single-shot fiber diffraction using a femtosecond X-ray free-electron laser
    journal, June 2017

    • Popp, David; Loh, N. Duane; Zorgati, Habiba
    • Cytoskeleton, Vol. 74, Issue 12
    • DOI: 10.1002/cm.21378

    Modulation of Microtubule Interprotofilament Interactions by Modified Taxanes
    journal, December 2011

    • Matesanz, Ruth; Rodríguez-Salarichs, Javier; Pera, Benet
    • Biophysical Journal, Vol. 101, Issue 12
    • DOI: 10.1016/j.bpj.2011.11.005

    Mechanistic Origin of Microtubule Dynamic Instability and Its Modulation by EB Proteins
    journal, August 2015


    Microtubule Assembly Dynamics at the Nanoscale
    journal, September 2007


    Maximum-likelihood Multi-reference Refinement for Electron Microscopy Images
    journal, April 2005

    • Scheres, Sjors H. W.; Valle, Mikel; Nuñez, Rafael
    • Journal of Molecular Biology, Vol. 348, Issue 1
    • DOI: 10.1016/j.jmb.2005.02.031

    Insights into the Distinct Mechanisms of Action of Taxane and Non-Taxane Microtubule Stabilizers from Cryo-EM Structures
    journal, March 2017

    • Kellogg, Elizabeth H.; Hejab, Nisreen M. A.; Howes, Stuart
    • Journal of Molecular Biology, Vol. 429, Issue 5
    • DOI: 10.1016/j.jmb.2017.01.001

    XMIPP: a new generation of an open-source image processing package for electron microscopy
    journal, November 2004

    • Sorzano, C. O. S.; Marabini, R.; Velázquez-Muriel, J.
    • Journal of Structural Biology, Vol. 148, Issue 2
    • DOI: 10.1016/j.jsb.2004.06.006

    How cryo-EM is revolutionizing structural biology
    journal, January 2015

    • Bai, Xiao-chen; McMullan, Greg; Scheres, Sjors H. W.
    • Trends in Biochemical Sciences, Vol. 40, Issue 1
    • DOI: 10.1016/j.tibs.2014.10.005

    The 4 Å X-Ray Structure of a Tubulin:Stathmin-like Domain Complex
    journal, September 2000


    How does taxol stabilize microtubules?
    journal, August 1995


    Purification of brain tubulin through two cycles of polymerization–depolymerization in a high-molarity buffer
    journal, November 2003


    Realizing the potential of electron cryo-microscopy
    journal, February 2004


    X-ray kinetic studies of microtubule assembly using synchrotron radiation
    journal, October 1980

    • Mandelkow, Eva-Maria; Harmsen, Arnold; Mandelkow, Eckhard
    • Nature, Vol. 287, Issue 5783
    • DOI: 10.1038/287595a0

    Dynamic instability of microtubule growth
    journal, November 1984

    • Mitchison, Tim; Kirschner, Marc
    • Nature, Vol. 312, Issue 5991
    • DOI: 10.1038/312237a0

    Structure of the αβ tubulin dimer by electron crystallography
    journal, January 1998

    • Nogales, Eva; Wolf, Sharon G.; Downing, Kenneth H.
    • Nature, Vol. 391, Issue 6663
    • DOI: 10.1038/34465

    Potential for biomolecular imaging with femtosecond X-ray pulses
    journal, August 2000

    • Neutze, Richard; Wouts, Remco; van der Spoel, David
    • Nature, Vol. 406, Issue 6797
    • DOI: 10.1038/35021099

    Mitosis: a history of division
    journal, January 2001

    • Mitchison, T. J.; Salmon, E. D.
    • Nature Cell Biology, Vol. 3, Issue 1
    • DOI: 10.1038/35050656

    Structure of tubulin at 6.5 Å and location of the taxol-binding site
    journal, June 1995

    • Nogales, Eva; Grayer Wolf, Sharon; Khan, Israr A.
    • Nature, Vol. 375, Issue 6530
    • DOI: 10.1038/375424a0

    Femtosecond time-delay X-ray holography
    journal, August 2007

    • Chapman, Henry N.; Hau-Riege, Stefan P.; Bogan, Michael J.
    • Nature, Vol. 448, Issue 7154
    • DOI: 10.1038/nature06049

    The Ndc80 kinetochore complex forms oligomeric arrays along microtubules
    journal, October 2010

    • Alushin, Gregory M.; Ramey, Vincent H.; Pasqualato, Sebastiano
    • Nature, Vol. 467, Issue 7317
    • DOI: 10.1038/nature09423

    Single mimivirus particles intercepted and imaged with an X-ray laser
    journal, February 2011

    • Seibert, M. Marvin; Ekeberg, Tomas; Maia, Filipe R. N. C.
    • Nature, Vol. 470, Issue 7332
    • DOI: 10.1038/nature09748

    Femtosecond X-ray protein nanocrystallography
    journal, February 2011

    • Chapman, Henry N.; Fromme, Petra; Barty, Anton
    • Nature, Vol. 470, Issue 7332, p. 73-77
    • DOI: 10.1038/nature09750

    The Coherent X-ray Imaging Data Bank
    journal, August 2012


    First lasing and operation of an ångstrom-wavelength free-electron laser
    journal, August 2010


    Image processing for electron microscopy single-particle analysis using XMIPP
    journal, May 2008

    • Scheres, Sjors H. W.; Núñez-Ramírez, Rafael; Sorzano, Carlos O. S.
    • Nature Protocols, Vol. 3, Issue 6
    • DOI: 10.1038/nprot.2008.62

    Microtubule-binding agents: a dynamic field of cancer therapeutics
    journal, October 2010

    • Dumontet, Charles; Jordan, Mary Ann
    • Nature Reviews Drug Discovery, Vol. 9, Issue 10
    • DOI: 10.1038/nrd3253

    Insight into microtubule disassembly by kinesin-13s from the structure of Kif2C bound to tubulin
    journal, July 2017


    Femtosecond X-ray coherent diffraction of aligned amyloid fibrils on low background graphene
    journal, May 2018

    • Seuring, Carolin; Ayyer, Kartik; Filippaki, Eleftheria
    • Nature Communications, Vol. 9, Article No. 1836
    • DOI: 10.1038/s41467-018-04116-9

    Visualization of a Mammalian Mitochondrion by Coherent X-ray Diffractive Imaging
    journal, May 2017


    A data set from flash X-ray imaging of carboxysomes
    journal, August 2016

    • Hantke, Max F.; Hasse, Dirk; Ekeberg, Tomas
    • Scientific Data, Vol. 3, Issue 1
    • DOI: 10.1038/sdata.2016.61

    Coherent diffraction of single Rice Dwarf virus particles using hard X-rays at the Linac Coherent Light Source
    journal, August 2016

    • Munke, Anna; Andreasson, Jakob; Aquila, Andrew
    • Scientific Data, Vol. 3, Issue 1
    • DOI: 10.1038/sdata.2016.64

    Fixed-target protein serial microcrystallography with an x-ray free electron laser
    journal, August 2014

    • Hunter, Mark S.; Segelke, Brent; Messerschmidt, Marc
    • Scientific Reports, Vol. 4, Issue 1
    • DOI: 10.1038/srep06026

    Injector for scattering measurements on fully solvated biospecies
    journal, March 2012

    • Weierstall, U.; Spence, J. C. H.; Doak, R. B.
    • Review of Scientific Instruments, Vol. 83, Issue 3
    • DOI: 10.1063/1.3693040

    The linac coherent light source single particle imaging road map
    journal, July 2015

    • Aquila, A.; Barty, A.; Bostedt, C.
    • Structural Dynamics, Vol. 2, Issue 4
    • DOI: 10.1063/1.4918726

    Near-atomic cryo-EM structure of PRC1 bound to the microtubule
    journal, August 2016

    • Kellogg, Elizabeth H.; Howes, Stuart; Ti, Shih-Chieh
    • Proceedings of the National Academy of Sciences, Vol. 113, Issue 34
    • DOI: 10.1073/pnas.1609903113

    Microtubule Assembly in the Absence of Added Nucleotides
    journal, March 1973

    • Shelanski, M. L.; Gaskin, F.; Cantor, C. R.
    • Proceedings of the National Academy of Sciences, Vol. 70, Issue 3
    • DOI: 10.1073/pnas.70.3.765

    Microtubule structure at low resolution by x-ray diffraction.
    journal, August 1977

    • Mandelkow, E.; Thomas, J.; Cohen, C.
    • Proceedings of the National Academy of Sciences, Vol. 74, Issue 8
    • DOI: 10.1073/pnas.74.8.3370

    Structural changes at microtubule ends accompanying GTP hydrolysis: Information from a slowly hydrolyzable analogue of GTP, guanylyl ( , )methylenediphosphonate
    journal, March 1998

    • Muller-Reichert, T.; Chretien, D.; Severin, F.
    • Proceedings of the National Academy of Sciences, Vol. 95, Issue 7
    • DOI: 10.1073/pnas.95.7.3661

    Microtubules: Evidence for 13 Protofilaments
    journal, November 1973


    Gas dynamic virtual nozzle for generation of microscopic droplet streams
    journal, September 2008

    • DePonte, D. P.; Weierstall, U.; Schmidt, K.
    • Journal of Physics D: Applied Physics, Vol. 41, Issue 19, Article No. 195505
    • DOI: 10.1088/0022-3727/41/19/195505

    Mitosis
    journal, September 2016


    Three-Dimensional Reconstruction of the Giant Mimivirus Particle with an X-Ray Free-Electron Laser
    journal, March 2015


    Cheetah : software for high-throughput reduction and analysis of serial femtosecond X-ray diffraction data
    journal, May 2014

    • Barty, Anton; Kirian, Richard A.; Maia, Filipe R. N. C.
    • Journal of Applied Crystallography, Vol. 47, Issue 3
    • DOI: 10.1107/s1600576714007626

    Analysis of XFEL serial diffraction data from individual crystalline fibrils
    journal, October 2017


    Considerations for three-dimensional image reconstruction from experimental data in coherent diffractive imaging
    journal, September 2018


    The CSPAD megapixel x-ray camera at LCLS
    conference, October 2012

    • Hart, Philip; Boutet, Sébastien; Carini, Gabriella
    • SPIE Optical Engineering + Applications, SPIE Proceedings
    • DOI: 10.1117/12.930924

    High-Resolution Protein Structure Determination by Serial Femtosecond Crystallography
    journal, May 2012


    Structural Basis for Microtubule Binding and Release by Dynein
    journal, September 2012


    Molecular Mechanism of Action of Microtubule-Stabilizing Anticancer Agents
    journal, January 2013


    Near-atomic model of microtubule-tau interactions
    journal, May 2018

    • Kellogg, Elizabeth H.; Hejab, Nisreen M. A.; Poepsel, Simon
    • Science, Vol. 360, Issue 6394
    • DOI: 10.1126/science.aat1780

    Microtubule Polymerization Dynamics
    journal, November 1997


    Reconstruction of an object from the modulus of its Fourier transform
    journal, January 1978


    Coherent diffractive imaging of microtubules using an X-ray laser
    text, January 2019

    • Brändén, Gisela; Hammarin, Greger; Harimoorthy, Rajiv
    • Deutsches Elektronen-Synchrotron, DESY, Hamburg
    • DOI: 10.3204/pubdb-2019-03073

      Figures/Tables have been extracted from DOE-funded journal article accepted manuscripts.