skip to main content
DOE PAGES title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Electrospray sample injection for single-particle imaging with x-ray lasers

Abstract

The possibility of imaging single proteins constitutes an exciting challenge for x-ray lasers. Despite encouraging results on large particles, imaging small particles has proven to be difficult for two reasons: not quite high enough pulse intensity from currently available x-ray lasers and, as we demonstrate here, contamination of the aerosolized molecules by nonvolatile contaminants in the solution. The amount of contamination on the sample depends on the initial droplet size during aerosolization. Here, we show that, with our electrospray injector, we can decrease the size of aerosol droplets and demonstrate virtually contaminant-free sample delivery of organelles, small virions, and proteins. The results presented here, together with the increased performance of next-generation x-ray lasers, constitute an important stepping stone toward the ultimate goal of protein structure determination from imaging at room temperature and high temporal resolution.

Authors:
ORCiD logo [1]; ORCiD logo [2]; ORCiD logo [3];  [4]; ORCiD logo [4]; ORCiD logo [4]; ORCiD logo [4];  [5]; ORCiD logo [4]; ORCiD logo [5];  [6]; ORCiD logo [4]; ORCiD logo [4];  [4]; ORCiD logo [4];  [4]; ORCiD logo [4];  [4];  [7]; ORCiD logo [8] more »; ORCiD logo [9]; ORCiD logo [10];  [11];  [12];  [11]; ORCiD logo [13];  [4];  [11];  [11]; ORCiD logo [11]; ORCiD logo [14];  [15];  [11];  [16];  [11]; ORCiD logo [4]; ORCiD logo [17]; ORCiD logo [18]; ORCiD logo [19] « less
  1. Uppsala Univ., Uppsala (Sweden); European XFEL GmbH, Schenefeld (Germany)
  2. Uppsala Univ., Uppsala (Sweden); Oxford Univ., Oxford (United Kingdom); Refeyn Ltd., Oxford (United Kingdom)
  3. Uppsala Univ., Uppsala (Sweden); National Univ. of Singapore (Singapore)
  4. Uppsala Univ., Uppsala (Sweden)
  5. Uppsala Univ., Uppsala (Sweden); KTH Royal Institute of Technology, Stockholm (Sweden)
  6. La Trobe Univ., Melbourne, VIC (Australia)
  7. Academy of Sciences of the Czech Republic, Prague (Czech Republic)
  8. Osaka Univ., Osaka (Japan); Hiroshima Univ., Hiroshima (Japan)
  9. Uppsala Univ., Uppsala (Sweden); Utrecht Univ., Utrecht (Netherlands)
  10. National Univ. of Singapore (Singapore)
  11. SLAC National Accelerator Lab., Menlo Park, CA (United States)
  12. Argonne National Lab. (ANL), Argonne, IL (United States); Northwestern Univ., Evanston, IL (United States)
  13. SLAC National Accelerator Lab., Menlo Park, CA (United States); Leibniz Univ. Hannover, Hannover (Germany)
  14. Argonne National Lab. (ANL), Argonne, IL (United States)
  15. Technische Univ. Berlin, Berlin (Germany)
  16. SLAC National Accelerator Lab., Menlo Park, CA (United States); Brookhaven National Lab. (BNL), Upton, NY (United States)
  17. Academy of Sciences of the Czech Republic, Prague (Czech Republic); Chalmers Univ. of Technology, Gothenburg (Sweden)
  18. Uppsala Univ., Uppsala (Sweden); Academy of Sciences of the Czech Republic; Prague (Czech Republic)
  19. Uppsala Univ., Uppsala (Sweden); Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
Publication Date:
Research Org.:
SLAC National Accelerator Lab., Menlo Park, CA (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1532400
Grant/Contract Number:  
AC02-76SF00515
Resource Type:
Accepted Manuscript
Journal Name:
Science Advances
Additional Journal Information:
Journal Volume: 5; Journal Issue: 5; Journal ID: ISSN 2375-2548
Publisher:
AAAS
Country of Publication:
United States
Language:
English

Citation Formats

Bielecki, Johan, Hantke, Max F., Daurer, Benedikt J., Reddy, Hemanth K. N., Hasse, Dirk, Larsson, Daniel S. D., Gunn, Laura H., Svenda, Martin, Munke, Anna, Sellberg, Jonas A., Flueckiger, Leonie, Pietrini, Alberto, Nettelblad, Carl, Lundholm, Ida, Carlsson, Gunilla, Okamoto, Kenta, Timneanu, Nicusor, Westphal, Daniel, Kulyk, Olena, Higashiura, Akifumi, van der Schot, Gijs, Loh, Ne-Te Duane, Wysong, Taylor E., Bostedt, Christoph, Gorkhover, Tais, Iwan, Bianca, Seibert, M. Marvin, Osipov, Timur, Walter, Peter, Hart, Philip, Bucher, Maximilian, Ulmer, Anatoli, Ray, Dipanwita, Carini, Gabriella, Ferguson, Ken R., Andersson, Inger, Andreasson, Jakob, Hajdu, Janos, and Maia, Filipe R. N. C. Electrospray sample injection for single-particle imaging with x-ray lasers. United States: N. p., 2019. Web. doi:10.1126/sciadv.aav8801.
Bielecki, Johan, Hantke, Max F., Daurer, Benedikt J., Reddy, Hemanth K. N., Hasse, Dirk, Larsson, Daniel S. D., Gunn, Laura H., Svenda, Martin, Munke, Anna, Sellberg, Jonas A., Flueckiger, Leonie, Pietrini, Alberto, Nettelblad, Carl, Lundholm, Ida, Carlsson, Gunilla, Okamoto, Kenta, Timneanu, Nicusor, Westphal, Daniel, Kulyk, Olena, Higashiura, Akifumi, van der Schot, Gijs, Loh, Ne-Te Duane, Wysong, Taylor E., Bostedt, Christoph, Gorkhover, Tais, Iwan, Bianca, Seibert, M. Marvin, Osipov, Timur, Walter, Peter, Hart, Philip, Bucher, Maximilian, Ulmer, Anatoli, Ray, Dipanwita, Carini, Gabriella, Ferguson, Ken R., Andersson, Inger, Andreasson, Jakob, Hajdu, Janos, & Maia, Filipe R. N. C. Electrospray sample injection for single-particle imaging with x-ray lasers. United States. doi:10.1126/sciadv.aav8801.
Bielecki, Johan, Hantke, Max F., Daurer, Benedikt J., Reddy, Hemanth K. N., Hasse, Dirk, Larsson, Daniel S. D., Gunn, Laura H., Svenda, Martin, Munke, Anna, Sellberg, Jonas A., Flueckiger, Leonie, Pietrini, Alberto, Nettelblad, Carl, Lundholm, Ida, Carlsson, Gunilla, Okamoto, Kenta, Timneanu, Nicusor, Westphal, Daniel, Kulyk, Olena, Higashiura, Akifumi, van der Schot, Gijs, Loh, Ne-Te Duane, Wysong, Taylor E., Bostedt, Christoph, Gorkhover, Tais, Iwan, Bianca, Seibert, M. Marvin, Osipov, Timur, Walter, Peter, Hart, Philip, Bucher, Maximilian, Ulmer, Anatoli, Ray, Dipanwita, Carini, Gabriella, Ferguson, Ken R., Andersson, Inger, Andreasson, Jakob, Hajdu, Janos, and Maia, Filipe R. N. C. Fri . "Electrospray sample injection for single-particle imaging with x-ray lasers". United States. doi:10.1126/sciadv.aav8801. https://www.osti.gov/servlets/purl/1532400.
@article{osti_1532400,
title = {Electrospray sample injection for single-particle imaging with x-ray lasers},
author = {Bielecki, Johan and Hantke, Max F. and Daurer, Benedikt J. and Reddy, Hemanth K. N. and Hasse, Dirk and Larsson, Daniel S. D. and Gunn, Laura H. and Svenda, Martin and Munke, Anna and Sellberg, Jonas A. and Flueckiger, Leonie and Pietrini, Alberto and Nettelblad, Carl and Lundholm, Ida and Carlsson, Gunilla and Okamoto, Kenta and Timneanu, Nicusor and Westphal, Daniel and Kulyk, Olena and Higashiura, Akifumi and van der Schot, Gijs and Loh, Ne-Te Duane and Wysong, Taylor E. and Bostedt, Christoph and Gorkhover, Tais and Iwan, Bianca and Seibert, M. Marvin and Osipov, Timur and Walter, Peter and Hart, Philip and Bucher, Maximilian and Ulmer, Anatoli and Ray, Dipanwita and Carini, Gabriella and Ferguson, Ken R. and Andersson, Inger and Andreasson, Jakob and Hajdu, Janos and Maia, Filipe R. N. C.},
abstractNote = {The possibility of imaging single proteins constitutes an exciting challenge for x-ray lasers. Despite encouraging results on large particles, imaging small particles has proven to be difficult for two reasons: not quite high enough pulse intensity from currently available x-ray lasers and, as we demonstrate here, contamination of the aerosolized molecules by nonvolatile contaminants in the solution. The amount of contamination on the sample depends on the initial droplet size during aerosolization. Here, we show that, with our electrospray injector, we can decrease the size of aerosol droplets and demonstrate virtually contaminant-free sample delivery of organelles, small virions, and proteins. The results presented here, together with the increased performance of next-generation x-ray lasers, constitute an important stepping stone toward the ultimate goal of protein structure determination from imaging at room temperature and high temporal resolution.},
doi = {10.1126/sciadv.aav8801},
journal = {Science Advances},
number = 5,
volume = 5,
place = {United States},
year = {2019},
month = {5}
}

Journal Article:
Free Publicly Available Full Text
Publisher's Version of Record

Save / Share:

Works referenced in this record:

Single Particle X-ray Diffractive Imaging
journal, January 2008

  • Bogan, Michael J.; Benner, W. Henry; Boutet, Sébastien
  • Nano Letters, Vol. 8, Issue 1, p. 310-316
  • DOI: 10.1021/nl072728k

Gas dynamic virtual nozzle for generation of microscopic droplet streams
journal, September 2008

  • DePonte, D. P.; Weierstall, U.; Schmidt, K.
  • Journal of Physics D: Applied Physics, Vol. 41, Issue 19, Article No. 195505
  • DOI: 10.1088/0022-3727/41/19/195505

Correlations in Scattered X-Ray Laser Pulses Reveal Nanoscale Structural Features of Viruses
journal, October 2017

  • Kurta, Ruslan P.; Donatelli, Jeffrey J.; Yoon, Chun Hong
  • Physical Review Letters, Vol. 119, Issue 15, Article No. 158102
  • DOI: 10.1103/PhysRevLett.119.158102

Experimental strategies for imaging bioparticles with femtosecond hard X-ray pulses
journal, April 2017


Electrospray ionization for mass spectrometry of large biomolecules
journal, October 1989


Electrospraying of conducting liquids for monodisperse aerosol generation in the 4 nm to 1.8 μm diameter range
journal, September 1995

  • Chen, Da-Ren; Pui, David Y. H.; Kaufman, Stanley L.
  • Journal of Aerosol Science, Vol. 26, Issue 6, p. 963-977
  • DOI: 10.1016/0021-8502(95)00027-A