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Title: Exometabolomic Analysis of Cross-Feeding Metabolites

Abstract

Microbial consortia have the potential to perform complex, industrially important tasks. The design of microbial consortia requires knowledge of the substrate preferences and metabolic outputs of each member, to allow understanding of potential interactions such as competition and beneficial metabolic exchange. Here, we used exometabolite profiling to follow the resource processing by a microbial co-culture of two biotechnologically relevant microbes, the bacterial cellulose degrader Cellulomonas fimi, and the oleaginous yeast Yarrowia lipolytica. We characterized the substrate preferences of the two strains on compounds typically found in lignocellulose hydrolysates. This allowed prediction that specific sugars resulting from hemicellulose polysaccharide degradation by C. fimi may serve as a cross-feeding metabolites to Y. lipolytica in co-culture. We also showed that products of ionic liquid-treated switchgrass lignocellulose degradation by C. fimi were channeled to Y. lipolytica in a co-culture. Additionally, we observed metabolites, such as shikimic acid accumulating in the co-culture supernatants, suggesting the potential for producing interesting co-products. Insights gained from characterizing the exometabolite profiles of individual and co-cultures of the two strains can help to refine this interaction, and guide strategies for making this an industrially viable co-culture to produce valuable products from lignocellulose material.

Authors:
 [1];  [1];  [1]
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  1. Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
Publication Date:
Research Org.:
Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
Sponsoring Org.:
USDOE Office of Science (SC)
OSTI Identifier:
1532280
Grant/Contract Number:  
AC02-05CH11231
Resource Type:
Accepted Manuscript
Journal Name:
Metabolites
Additional Journal Information:
Journal Volume: 7; Journal Issue: 4; Journal ID: ISSN 2218-1989
Publisher:
MDPI
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; exometabolomics; bioprocessing; microbial consortia; cross-feeding; Yarrowia lipolytica; Cellulomonas fimi; lignocellulose

Citation Formats

Lubbe, Andrea, Bowen, Benjamin P., and Northen, Trent. Exometabolomic Analysis of Cross-Feeding Metabolites. United States: N. p., 2017. Web. doi:10.3390/metabo7040050.
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Lubbe, Andrea, Bowen, Benjamin P., & Northen, Trent. Exometabolomic Analysis of Cross-Feeding Metabolites. United States. doi:10.3390/metabo7040050.
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Lubbe, Andrea, Bowen, Benjamin P., and Northen, Trent. Wed . "Exometabolomic Analysis of Cross-Feeding Metabolites". United States. doi:10.3390/metabo7040050. https://www.osti.gov/servlets/purl/1532280.
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@article{osti_1532280,
title = {Exometabolomic Analysis of Cross-Feeding Metabolites},
author = {Lubbe, Andrea and Bowen, Benjamin P. and Northen, Trent},
abstractNote = {Microbial consortia have the potential to perform complex, industrially important tasks. The design of microbial consortia requires knowledge of the substrate preferences and metabolic outputs of each member, to allow understanding of potential interactions such as competition and beneficial metabolic exchange. Here, we used exometabolite profiling to follow the resource processing by a microbial co-culture of two biotechnologically relevant microbes, the bacterial cellulose degrader Cellulomonas fimi, and the oleaginous yeast Yarrowia lipolytica. We characterized the substrate preferences of the two strains on compounds typically found in lignocellulose hydrolysates. This allowed prediction that specific sugars resulting from hemicellulose polysaccharide degradation by C. fimi may serve as a cross-feeding metabolites to Y. lipolytica in co-culture. We also showed that products of ionic liquid-treated switchgrass lignocellulose degradation by C. fimi were channeled to Y. lipolytica in a co-culture. Additionally, we observed metabolites, such as shikimic acid accumulating in the co-culture supernatants, suggesting the potential for producing interesting co-products. Insights gained from characterizing the exometabolite profiles of individual and co-cultures of the two strains can help to refine this interaction, and guide strategies for making this an industrially viable co-culture to produce valuable products from lignocellulose material.},
doi = {10.3390/metabo7040050},
journal = {Metabolites},
number = 4,
volume = 7,
place = {United States},
year = {2017},
month = {10}
}
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DOI:  10.3390/metabo7040050
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