Combining loss of function of FOLYLPOLYGLUTAMATE SYNTHETASE1 and CAFFEOYL-COA 3-O-METHYLTRANSFERASE1 for lignin reduction and improved saccharification efficiency in Arabidopsis thaliana
Abstract
Background: Downregulation of genes involved in lignin biosynthesis and related biochemical pathways has been used as a strategy to improve biofuel production. Plant C1 metabolism provides the methyl units used for the methylation reactions carried out by two methyltransferases in the lignin biosynthetic pathway: caffeic acid 3-O-methyltransferase (COMT) and caffeoyl-CoA 3-O-methyltransferase (CCoAOMT). Mutations in these genes resulted in lower lignin levels and altered lignin compositions. Reduced lignin levels can also be achieved by mutations in the C1 pathway gene, fpgs1ccoaomt1 double mutants were generated and functionally characterized.Results: Double fpgs1ccoaomt1 mutants had lower thioacidolysis lignin monomer yield and acetyl bromide lignin content than the ccoaomt1 or fpgs1 mutants and the plants themselves displayed no obvious long-term negative growth phenotypes. Moreover, extracts from the double mutants had dramatically improved enzymatic polysaccharide hydrolysis efficiencies than the single mutants: 15.1% and 20.7% higher than ccoaomt1 and fpgs1, respectively. The reduced lignin and improved sugar release of fpgs1ccoaomt1 was coupled with changes in cell-wall composition, metabolite profiles, and changes in expression of genes involved in cell-wall and lignin biosynthesis. Conclusion: Our observations demonstrate that additional reduction in lignin content and improved sugar release can be achieved by simultaneous downregulation of a gene in the C1more »
- Authors:
- Publication Date:
- Research Org.:
- Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1618752
- Alternate Identifier(s):
- OSTI ID: 1531204
- Grant/Contract Number:
- AC05-00OR22725
- Resource Type:
- Published Article
- Journal Name:
- Biotechnology for Biofuels
- Additional Journal Information:
- Journal Name: Biotechnology for Biofuels Journal Volume: 12 Journal Issue: 1; Journal ID: ISSN 1754-6834
- Publisher:
- Springer Science + Business Media
- Country of Publication:
- Netherlands
- Language:
- English
- Subject:
- 09 BIOMASS FUELS
Citation Formats
Xie, Hongli, Engle, Nancy L., Venketachalam, Sivasankari, Yoo, Chang Geun, Barros, Jaime, Lecoultre, Mitch, Howard, Nikki, Li, Guifen, Sun, Liang, Srivastava, Avinash C., Pattathil, Sivakumar, Pu, Yunqiao, Hahn, Michael G., Ragauskas, Arthur J., Nelson, Richard S., Dixon, Richard A., Tschaplinski, Timothy J., Blancaflor, Elison B., and Tang, Yuhong. Combining loss of function of FOLYLPOLYGLUTAMATE SYNTHETASE1 and CAFFEOYL-COA 3-O-METHYLTRANSFERASE1 for lignin reduction and improved saccharification efficiency in Arabidopsis thaliana. Netherlands: N. p., 2019.
Web. doi:10.1186/s13068-019-1446-3.
Xie, Hongli, Engle, Nancy L., Venketachalam, Sivasankari, Yoo, Chang Geun, Barros, Jaime, Lecoultre, Mitch, Howard, Nikki, Li, Guifen, Sun, Liang, Srivastava, Avinash C., Pattathil, Sivakumar, Pu, Yunqiao, Hahn, Michael G., Ragauskas, Arthur J., Nelson, Richard S., Dixon, Richard A., Tschaplinski, Timothy J., Blancaflor, Elison B., & Tang, Yuhong. Combining loss of function of FOLYLPOLYGLUTAMATE SYNTHETASE1 and CAFFEOYL-COA 3-O-METHYLTRANSFERASE1 for lignin reduction and improved saccharification efficiency in Arabidopsis thaliana. Netherlands. https://doi.org/10.1186/s13068-019-1446-3
Xie, Hongli, Engle, Nancy L., Venketachalam, Sivasankari, Yoo, Chang Geun, Barros, Jaime, Lecoultre, Mitch, Howard, Nikki, Li, Guifen, Sun, Liang, Srivastava, Avinash C., Pattathil, Sivakumar, Pu, Yunqiao, Hahn, Michael G., Ragauskas, Arthur J., Nelson, Richard S., Dixon, Richard A., Tschaplinski, Timothy J., Blancaflor, Elison B., and Tang, Yuhong. Fri .
"Combining loss of function of FOLYLPOLYGLUTAMATE SYNTHETASE1 and CAFFEOYL-COA 3-O-METHYLTRANSFERASE1 for lignin reduction and improved saccharification efficiency in Arabidopsis thaliana". Netherlands. https://doi.org/10.1186/s13068-019-1446-3.
@article{osti_1618752,
title = {Combining loss of function of FOLYLPOLYGLUTAMATE SYNTHETASE1 and CAFFEOYL-COA 3-O-METHYLTRANSFERASE1 for lignin reduction and improved saccharification efficiency in Arabidopsis thaliana},
author = {Xie, Hongli and Engle, Nancy L. and Venketachalam, Sivasankari and Yoo, Chang Geun and Barros, Jaime and Lecoultre, Mitch and Howard, Nikki and Li, Guifen and Sun, Liang and Srivastava, Avinash C. and Pattathil, Sivakumar and Pu, Yunqiao and Hahn, Michael G. and Ragauskas, Arthur J. and Nelson, Richard S. and Dixon, Richard A. and Tschaplinski, Timothy J. and Blancaflor, Elison B. and Tang, Yuhong},
abstractNote = {Background: Downregulation of genes involved in lignin biosynthesis and related biochemical pathways has been used as a strategy to improve biofuel production. Plant C1 metabolism provides the methyl units used for the methylation reactions carried out by two methyltransferases in the lignin biosynthetic pathway: caffeic acid 3-O-methyltransferase (COMT) and caffeoyl-CoA 3-O-methyltransferase (CCoAOMT). Mutations in these genes resulted in lower lignin levels and altered lignin compositions. Reduced lignin levels can also be achieved by mutations in the C1 pathway gene, fpgs1ccoaomt1 double mutants were generated and functionally characterized.Results: Double fpgs1ccoaomt1 mutants had lower thioacidolysis lignin monomer yield and acetyl bromide lignin content than the ccoaomt1 or fpgs1 mutants and the plants themselves displayed no obvious long-term negative growth phenotypes. Moreover, extracts from the double mutants had dramatically improved enzymatic polysaccharide hydrolysis efficiencies than the single mutants: 15.1% and 20.7% higher than ccoaomt1 and fpgs1, respectively. The reduced lignin and improved sugar release of fpgs1ccoaomt1 was coupled with changes in cell-wall composition, metabolite profiles, and changes in expression of genes involved in cell-wall and lignin biosynthesis. Conclusion: Our observations demonstrate that additional reduction in lignin content and improved sugar release can be achieved by simultaneous downregulation of a gene in the C1 (FPGS1) and lignin biosynthetic (CCOAOMT) pathways. These improvements in sugar accessibility were achieved without introducing unwanted long-term plant growth and developmental defects.},
doi = {10.1186/s13068-019-1446-3},
journal = {Biotechnology for Biofuels},
number = 1,
volume = 12,
place = {Netherlands},
year = {Fri May 03 00:00:00 EDT 2019},
month = {Fri May 03 00:00:00 EDT 2019}
}
https://doi.org/10.1186/s13068-019-1446-3
Web of Science
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