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Title: Combining loss of function of FOLYLPOLYGLUTAMATE SYNTHETASE1 and CAFFEOYL-COA 3-O-METHYLTRANSFERASE1 for lignin reduction and improved saccharification efficiency in Arabidopsis thaliana

Abstract

Background: Downregulation of genes involved in lignin biosynthesis and related biochemical pathways has been used as a strategy to improve biofuel production. Plant C1 metabolism provides the methyl units used for the methylation reactions carried out by two methyltransferases in the lignin biosynthetic pathway: caffeic acid 3-O-methyltransferase (COMT) and caffeoyl-CoA 3-O-methyltransferase (CCoAOMT). Mutations in these genes resulted in lower lignin levels and altered lignin compositions. Reduced lignin levels can also be achieved by mutations in the C1 pathway gene, fpgs1ccoaomt1 double mutants were generated and functionally characterized.Results: Double fpgs1ccoaomt1 mutants had lower thioacidolysis lignin monomer yield and acetyl bromide lignin content than the ccoaomt1 or fpgs1 mutants and the plants themselves displayed no obvious long-term negative growth phenotypes. Moreover, extracts from the double mutants had dramatically improved enzymatic polysaccharide hydrolysis efficiencies than the single mutants: 15.1% and 20.7% higher than ccoaomt1 and fpgs1, respectively. The reduced lignin and improved sugar release of fpgs1ccoaomt1 was coupled with changes in cell-wall composition, metabolite profiles, and changes in expression of genes involved in cell-wall and lignin biosynthesis. Conclusion: Our observations demonstrate that additional reduction in lignin content and improved sugar release can be achieved by simultaneous downregulation of a gene in the C1more » (FPGS1) and lignin biosynthetic (CCOAOMT) pathways. These improvements in sugar accessibility were achieved without introducing unwanted long-term plant growth and developmental defects.« less

Authors:
 [1]; ORCiD logo [2];  [3];  [2];  [4];  [1];  [1];  [5];  [5];  [1];  [6]; ORCiD logo [2];  [3]; ORCiD logo [2];  [1];  [4]; ORCiD logo [2];  [1]; ORCiD logo [1]
  1. Noble Research Inst., LLC, Ardmore, OK (United States); Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). BioEnergy Science Center
  2. Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). Biosciences Division; Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). BioEnergy Science Center; Dept. of Energy (DOE), Oak Ridge, TN (United States). Center for Bioenergy Innovation
  3. Univ. of Georgia, Athens, GA (United States). Complex Carbohydrate Research Center; Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). BioEnergy Science Center; Dept. of Energy (DOE), Oak Ridge, TN (United States). Center for Bioenergy Innovation
  4. Univ. of North Texas, Denton, TX (United States). BioDiscovery Inst. and Dept. of Biological Sciences; Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). BioEnergy Science Center; Dept. of Energy (DOE), Oak Ridge, TN (United States). Center for Bioenergy Innovation
  5. Noble Research Inst., LLC, Ardmore, OK (United States)
  6. Univ. of Georgia, Athens, GA (United States). Complex Carbohydrate Research Center; Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). BioEnergy Science Center
Publication Date:
Research Org.:
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1531204
Grant/Contract Number:  
AC05-00OR22725
Resource Type:
Accepted Manuscript
Journal Name:
Biotechnology for Biofuels
Additional Journal Information:
Journal Volume: 12; Journal Issue: 1; Journal ID: ISSN 1754-6834
Publisher:
BioMed Central
Country of Publication:
United States
Language:
English
Subject:
09 BIOMASS FUELS

Citation Formats

Xie, Hongli, Engle, Nancy L., Venketachalam, Sivasankari, Yoo, Chang Geun, Barros, Jaime, Lecoultre, Mitch, Howard, Nikki, Li, Guifen, Sun, Liang, Srivastava, Avinash C., Pattathil, Sivakumar, Pu, Yunqiao, Hahn, Michael G., Ragauskas, Arthur J., Nelson, Richard S., Dixon, Richard A., Tschaplinski, Timothy J., Blancaflor, Elison B., and Tang, Yuhong. Combining loss of function of FOLYLPOLYGLUTAMATE SYNTHETASE1 and CAFFEOYL-COA 3-O-METHYLTRANSFERASE1 for lignin reduction and improved saccharification efficiency in Arabidopsis thaliana. United States: N. p., 2019. Web. doi:10.1186/s13068-019-1446-3.
Xie, Hongli, Engle, Nancy L., Venketachalam, Sivasankari, Yoo, Chang Geun, Barros, Jaime, Lecoultre, Mitch, Howard, Nikki, Li, Guifen, Sun, Liang, Srivastava, Avinash C., Pattathil, Sivakumar, Pu, Yunqiao, Hahn, Michael G., Ragauskas, Arthur J., Nelson, Richard S., Dixon, Richard A., Tschaplinski, Timothy J., Blancaflor, Elison B., & Tang, Yuhong. Combining loss of function of FOLYLPOLYGLUTAMATE SYNTHETASE1 and CAFFEOYL-COA 3-O-METHYLTRANSFERASE1 for lignin reduction and improved saccharification efficiency in Arabidopsis thaliana. United States. doi:10.1186/s13068-019-1446-3.
Xie, Hongli, Engle, Nancy L., Venketachalam, Sivasankari, Yoo, Chang Geun, Barros, Jaime, Lecoultre, Mitch, Howard, Nikki, Li, Guifen, Sun, Liang, Srivastava, Avinash C., Pattathil, Sivakumar, Pu, Yunqiao, Hahn, Michael G., Ragauskas, Arthur J., Nelson, Richard S., Dixon, Richard A., Tschaplinski, Timothy J., Blancaflor, Elison B., and Tang, Yuhong. Fri . "Combining loss of function of FOLYLPOLYGLUTAMATE SYNTHETASE1 and CAFFEOYL-COA 3-O-METHYLTRANSFERASE1 for lignin reduction and improved saccharification efficiency in Arabidopsis thaliana". United States. doi:10.1186/s13068-019-1446-3. https://www.osti.gov/servlets/purl/1531204.
@article{osti_1531204,
title = {Combining loss of function of FOLYLPOLYGLUTAMATE SYNTHETASE1 and CAFFEOYL-COA 3-O-METHYLTRANSFERASE1 for lignin reduction and improved saccharification efficiency in Arabidopsis thaliana},
author = {Xie, Hongli and Engle, Nancy L. and Venketachalam, Sivasankari and Yoo, Chang Geun and Barros, Jaime and Lecoultre, Mitch and Howard, Nikki and Li, Guifen and Sun, Liang and Srivastava, Avinash C. and Pattathil, Sivakumar and Pu, Yunqiao and Hahn, Michael G. and Ragauskas, Arthur J. and Nelson, Richard S. and Dixon, Richard A. and Tschaplinski, Timothy J. and Blancaflor, Elison B. and Tang, Yuhong},
abstractNote = {Background: Downregulation of genes involved in lignin biosynthesis and related biochemical pathways has been used as a strategy to improve biofuel production. Plant C1 metabolism provides the methyl units used for the methylation reactions carried out by two methyltransferases in the lignin biosynthetic pathway: caffeic acid 3-O-methyltransferase (COMT) and caffeoyl-CoA 3-O-methyltransferase (CCoAOMT). Mutations in these genes resulted in lower lignin levels and altered lignin compositions. Reduced lignin levels can also be achieved by mutations in the C1 pathway gene, fpgs1ccoaomt1 double mutants were generated and functionally characterized.Results: Double fpgs1ccoaomt1 mutants had lower thioacidolysis lignin monomer yield and acetyl bromide lignin content than the ccoaomt1 or fpgs1 mutants and the plants themselves displayed no obvious long-term negative growth phenotypes. Moreover, extracts from the double mutants had dramatically improved enzymatic polysaccharide hydrolysis efficiencies than the single mutants: 15.1% and 20.7% higher than ccoaomt1 and fpgs1, respectively. The reduced lignin and improved sugar release of fpgs1ccoaomt1 was coupled with changes in cell-wall composition, metabolite profiles, and changes in expression of genes involved in cell-wall and lignin biosynthesis. Conclusion: Our observations demonstrate that additional reduction in lignin content and improved sugar release can be achieved by simultaneous downregulation of a gene in the C1 (FPGS1) and lignin biosynthetic (CCOAOMT) pathways. These improvements in sugar accessibility were achieved without introducing unwanted long-term plant growth and developmental defects.},
doi = {10.1186/s13068-019-1446-3},
journal = {Biotechnology for Biofuels},
number = 1,
volume = 12,
place = {United States},
year = {2019},
month = {5}
}

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