Superresolution microscopy reveals linkages between ribosomal DNA on heterologous chromosomes

Potapova, Tamara A.; Unruh, Jay R.; Yu, Zulin; Rancati, Giulia; Li, Hua; Stampfer, Martha R.; Gerton, Jennifer L.

doi:10.1083/jcb.201810166

Title: Superresolution microscopy reveals linkages between ribosomal DNA on heterologous chromosomes

Journal Article · 02 July 2019 · Journal of Cell Biology

DOI: https://doi.org/10.1083/jcb.201810166 · OSTI ID:1530923

Potapova, Tamara A. ^[1];

^[1]; Yu, Zulin ^[1];

^[2]; Li, Hua ^[1];

^[3];

^[4]

Stowers Institute for Medical Research, Kansas City, MO
Institute of Medical Biology, Agency for Science, Technology and Research, Singapore
Biological Systems and Engineering Division, Lawrence Berkeley National Laboratory, Berkeley, CA
Stowers Institute for Medical Research, Kansas City, MO, Department of Biochemistry and Molecular Biology, University of Kansas Medical Center, Kansas City, KS

The spatial organization of the genome is enigmatic. Direct evidence of physical contacts between chromosomes and their visualization at nanoscale resolution has been limited. We used superresolution microscopy to demonstrate that ribosomal DNA (rDNA) can form linkages between chromosomes. We observed rDNA linkages in many different human cell types and demonstrated their resolution in anaphase. rDNA linkages are coated by the transcription factor UBF and their formation depends on UBF, indicating that they regularly occur between transcriptionally active loci. Overexpression of c-Myc increases rDNA transcription and the frequency of rDNA linkages, further suggesting that their formation depends on active transcription. Linkages persist in the absence of cohesion, but inhibition of topoisomerase II prevents their resolution in anaphase. We propose that linkages are topological intertwines occurring between transcriptionally active rDNA loci spatially colocated in the same nucleolar compartment. Our findings suggest that active DNA loci engage in physical interchromosomal connections that are an integral and pervasive feature of genome organization.

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Sponsoring Organization:: USDOE Office of Science (SC)

Grant/Contract Number:: AC02-05CH11231

OSTI ID:: 1530923

Alternate ID(s):: OSTI ID: 1580897

Journal Information:: Journal of Cell Biology, Journal Name: Journal of Cell Biology Journal Issue: 8 Vol. 218; ISSN 0021-9525

Publisher:: Rockefeller University PressCopyright Statement

Country of Publication:: United States

Language:: English

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