Enhanced Aquatic Respiration Associated With Mixing of Clearwater Tributary and Turbid Amazon River Waters
Abstract
When water bodies with unique biogeochemical constituents mix together there is potential for diverse responses by aquatic microbial communities and associated ecosystem functions. Here we evaluate bulk respiration rates under varying mixtures of turbid Amazon River water and two lowland tributaries—the Tapajós and Xingu rivers—based on O2 drawdown in dark rotating incubation chambers. Preliminary experiments containing 50% Tapajós River and 50% Amazon River water were performed during falling water. More detailed experiments containing 5%, 17%, 33%, and 50% tributary water were performed for both the Tapajós and Xingu rivers at 3 different rotation velocities (0, 0.22, and 0.66 m s-1) during the falling water period. Respiration rates were higher in the mixtures compared to both river endmembers in most experiments. For both the Xingu and Tapajós rivers a 17% mixture yielded maximal respiration rates that were 1.6 to 2.8 times faster than each tributary, respectively. The 50% mixtures, on the other hand, only yielded 1.4 to 1.9 times amplified respiration rates for the Tapajós and Xingu rivers, respectively, and in one case was respiration was 2.8 times lower in the 50% mixture compared to the tributary. We hypothesize that these enhanced respiration rates are driven, in part, by microbial primingmore »
- Authors:
-
- Pacific Northwest National Lab. (PNNL), Richland, WA (United States); Univ. of Washington, Seattle, WA (United States); Univ. of Florida, Gainesville, FL (United States)
- Univ. of Washington, Seattle, WA (United States); Univ. de São Paulo, Piracicaba (Brazil); Umeå Univ., Umeå (Sweden)
- Univ. of Washington, Seattle, WA (United States)
- Univ. of Florida, Gainesville, FL (United States)
- Publication Date:
- Research Org.:
- Pacific Northwest National Lab. (PNNL), Richland, WA (United States)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1530887
- Alternate Identifier(s):
- OSTI ID: 1530746
- Report Number(s):
- PNNL-SA-140620
Journal ID: ISSN 2296-6463
- Grant/Contract Number:
- AC0576RL01830; AC05-76RL01830
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Frontiers in Earth Science
- Additional Journal Information:
- Journal Volume: 7; Journal ID: ISSN 2296-6463
- Publisher:
- Frontiers Research Foundation
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 58 GEOSCIENCES; respiration; aquatic; priming; carbon; mixing; Amazon, aquatic, bacteria, biogeochemistry, carbon cycling, continuum, decomposition, ecosystem, ecotone, global change, gradients, interface, metabolism, microbes, microbial, organic matter, oxygen, priming, respiration, river, terrestial aquatic interface
Citation Formats
Ward, Nicholas D., Sawakuchi, Henrique O., Richey, Jeffrey E., Keil, Richard G., and Bianchi, Thomas S. Enhanced Aquatic Respiration Associated With Mixing of Clearwater Tributary and Turbid Amazon River Waters. United States: N. p., 2019.
Web. doi:10.3389/FEART.2019.00101.
Ward, Nicholas D., Sawakuchi, Henrique O., Richey, Jeffrey E., Keil, Richard G., & Bianchi, Thomas S. Enhanced Aquatic Respiration Associated With Mixing of Clearwater Tributary and Turbid Amazon River Waters. United States. https://doi.org/10.3389/FEART.2019.00101
Ward, Nicholas D., Sawakuchi, Henrique O., Richey, Jeffrey E., Keil, Richard G., and Bianchi, Thomas S. Wed .
"Enhanced Aquatic Respiration Associated With Mixing of Clearwater Tributary and Turbid Amazon River Waters". United States. https://doi.org/10.3389/FEART.2019.00101. https://www.osti.gov/servlets/purl/1530887.
@article{osti_1530887,
title = {Enhanced Aquatic Respiration Associated With Mixing of Clearwater Tributary and Turbid Amazon River Waters},
author = {Ward, Nicholas D. and Sawakuchi, Henrique O. and Richey, Jeffrey E. and Keil, Richard G. and Bianchi, Thomas S.},
abstractNote = {When water bodies with unique biogeochemical constituents mix together there is potential for diverse responses by aquatic microbial communities and associated ecosystem functions. Here we evaluate bulk respiration rates under varying mixtures of turbid Amazon River water and two lowland tributaries—the Tapajós and Xingu rivers—based on O2 drawdown in dark rotating incubation chambers. Preliminary experiments containing 50% Tapajós River and 50% Amazon River water were performed during falling water. More detailed experiments containing 5%, 17%, 33%, and 50% tributary water were performed for both the Tapajós and Xingu rivers at 3 different rotation velocities (0, 0.22, and 0.66 m s-1) during the falling water period. Respiration rates were higher in the mixtures compared to both river endmembers in most experiments. For both the Xingu and Tapajós rivers a 17% mixture yielded maximal respiration rates that were 1.6 to 2.8 times faster than each tributary, respectively. The 50% mixtures, on the other hand, only yielded 1.4 to 1.9 times amplified respiration rates for the Tapajós and Xingu rivers, respectively, and in one case was respiration was 2.8 times lower in the 50% mixture compared to the tributary. We hypothesize that these enhanced respiration rates are driven, in part, by microbial priming effects that have been previously identified on a molecular level for these rivers. The results of this study suggest that there may be a “sweet spot” for priming effects to occur in terms of the relative abundance of “priming” and “primed” substrates.},
doi = {10.3389/FEART.2019.00101},
journal = {Frontiers in Earth Science},
number = ,
volume = 7,
place = {United States},
year = {2019},
month = {5}
}
Figures / Tables:

Works referenced in this record:
Plumbing the Global Carbon Cycle: Integrating Inland Waters into the Terrestrial Carbon Budget
journal, February 2007
- Cole, J. J.; Prairie, Y. T.; Caraco, N. F.
- Ecosystems, Vol. 10, Issue 1
Seasonal and spatial variability of dissolved organic matter composition in the lower Amazon River
journal, November 2016
- Seidel, Michael; Dittmar, Thorsten; Ward, Nicholas D.
- Biogeochemistry, Vol. 131, Issue 3
Source to sink: Evolution of lignin composition in the Madre de Dios River system with connection to the Amazon basin and offshore: Lignin Evolution in Amazon
journal, May 2016
- Feng, Xiaojuan; Feakins, Sarah J.; Liu, Zongguang
- Journal of Geophysical Research: Biogeosciences, Vol. 121, Issue 5
Diversity and dynamics of free-living and particle-associated Betaproteobacteria and Actinobacteria in relation to phytoplankton and zooplankton communities
journal, June 2011
- Parveen, Bushra; Reveilliez, Jean-Philippe; Mary, Isabelle
- FEMS Microbiology Ecology, Vol. 77, Issue 3
Fate of the Amazon River dissolved organic matter in the tropical Atlantic Ocean: DOM IN THE AMAZON RIVER-OCEAN CONTINUUM
journal, May 2015
- Medeiros, Patricia M.; Seidel, Michael; Ward, Nicholas D.
- Global Biogeochemical Cycles, Vol. 29, Issue 5
Organic Carbon: Oxidation and Transport in the Amazon River
journal, March 1980
- Richey, J. E.; Brock, J. T.; Naiman, R. J.
- Science, Vol. 207, Issue 4437
Metagenomic and metatranscriptomic inventories of the lower Amazon River, May 2011
journal, September 2015
- Satinsky, Brandon M.; Fortunato, Caroline S.; Doherty, Mary
- Microbiome, Vol. 3, Issue 1
Impact of Wetland Decline on Decreasing Dissolved Organic Carbon Concentrations along the Mississippi River Continuum
journal, January 2017
- Duan, Shuiwang; He, Yuxiang; Kaushal, Sujay S.
- Frontiers in Marine Science, Vol. 3
Degradation of terrestrially derived macromolecules in the Amazon River
journal, May 2013
- Ward, Nicholas D.; Keil, Richard G.; Medeiros, Patricia M.
- Nature Geoscience, Vol. 6, Issue 7
Marine microbial community responses related to wetland carbon mobilization in the coastal zone: Coastal microbial dynamics
journal, December 2018
- Ward, Nicholas D.; Morrison, Elise S.; Liu, Yina
- Limnology and Oceanography Letters, Vol. 4, Issue 1
Where Carbon Goes When Water Flows: Carbon Cycling across the Aquatic Continuum
journal, January 2017
- Ward, Nicholas D.; Bianchi, Thomas S.; Medeiros, Patricia M.
- Frontiers in Marine Science, Vol. 4
Organic carbon decomposition rates controlled by water retention time across inland waters
journal, May 2016
- Catalán, Núria; Marcé, Rafael; Kothawala, Dolly N.
- Nature Geoscience, Vol. 9, Issue 7
Negative Priming Effect on Organic Matter Mineralisation in NE Atlantic Slope Sediments
journal, June 2013
- Gontikaki, Evangelia; Thornton, Barry; Huvenne, Veerle A. I.
- PLoS ONE, Vol. 8, Issue 6
Simultaneous quantification of aquatic ecosystem metabolism and reaeration using a Bayesian statistical model of oxygen dynamics
journal, March 2010
- Holtgrieve, Gordon W.; Schindler, Daniel E.; Branch, Trevor A.
- Limnology and Oceanography, Vol. 55, Issue 3
The River Continuum Concept
journal, January 1980
- Vannote, Robin L.; Minshall, G. Wayne; Cummins, Kenneth W.
- Canadian Journal of Fisheries and Aquatic Sciences, Vol. 37, Issue 1
The role of terrestrially derived organic carbon in the coastal ocean: A changing paradigm and the priming effect
journal, November 2011
- Bianchi, T. S.
- Proceedings of the National Academy of Sciences, Vol. 108, Issue 49
What happens to terrestrial organic matter in the ocean?
journal, November 1997
- Hedges, J. I.; Keil, R. G.; Benner, R.
- Organic Geochemistry, Vol. 27, Issue 5-6
Molecular Signatures of Biogeochemical Transformations in Dissolved Organic Matter from Ten World Rivers
journal, September 2016
- Riedel, Thomas; Zark, Maren; Vähätalo, Anssi V.
- Frontiers in Earth Science, Vol. 4
Bacterial Biogeography across the Amazon River-Ocean Continuum
journal, May 2017
- Doherty, Mary; Yager, Patricia L.; Moran, Mary Ann
- Frontiers in Microbiology, Vol. 8
Carbon Dioxide Emissions along the Lower Amazon River
journal, March 2017
- Sawakuchi, Henrique O.; Neu, Vania; Ward, Nicholas D.
- Frontiers in Marine Science, Vol. 4
The compositional evolution of dissolved and particulate organic matter along the lower Amazon River—Óbidos to the ocean
journal, December 2015
- Ward, Nicholas D.; Krusche, Alex V.; Sawakuchi, Henrique O.
- Marine Chemistry, Vol. 177
Patterns of Bacterial and Archaeal Gene Expression through the Lower Amazon River
journal, August 2017
- Satinsky, Brandon M.; Smith, Christa B.; Sharma, Shalabh
- Frontiers in Marine Science, Vol. 4
Sorptive fractionation of dissolved organic nitrogen and amino acids onto fine sediments within the Amazon Basin
journal, November 2001
- Aufdenkampe, Anthony K.; Hedges, John I.; Richey, Jeffery E.
- Limnology and Oceanography, Vol. 46, Issue 8
Oxygen depletion in the upper reach of the Pearl River estuary during a winter drought
journal, November 2006
- Dai, Minhan; Guo, Xianghui; Zhai, Weidong
- Marine Chemistry, Vol. 102, Issue 1-2
Global carbon dioxide emissions from inland waters
journal, November 2013
- Raymond, Peter A.; Hartmann, Jens; Lauerwald, Ronny
- Nature, Vol. 503, Issue 7476
Molecular-level changes of dissolved organic matter along the Amazon River-to-ocean continuum
journal, December 2015
- Seidel, Michael; Yager, Patricia L.; Ward, Nicholas D.
- Marine Chemistry, Vol. 177
Persistence of soil organic matter as an ecosystem property
journal, October 2011
- Schmidt, Michael W. I.; Torn, Margaret S.; Abiven, Samuel
- Nature, Vol. 478, Issue 7367
Velocity-amplified microbial respiration rates in the lower Amazon River: Amazon River respiration
journal, February 2018
- Ward, Nicholas D.; Sawakuchi, Henrique O.; Neu, Vania
- Limnology and Oceanography Letters, Vol. 3, Issue 3
Interactive effects on organic matter processing from soils to the ocean: are priming effects relevant in aquatic ecosystems?
journal, June 2018
- Bengtsson, Mia M.; Attermeyer, Katrin; Catalán, Núria
- Hydrobiologia, Vol. 822, Issue 1
Positive priming of terrestrially derived dissolved organic matter in a freshwater microcosm system: Priming of Dissolved Organic Matter
journal, July 2015
- Bianchi, Thomas S.; Thornton, Daniel C. O.; Yvon-Lewis, Shari A.
- Geophysical Research Letters, Vol. 42, Issue 13
Persistence of soil organic matter as an ecosystem property
text, January 2011
- Schmidt, M. W. I.; Torn, M. S.; Abiven, S.
- Nature Publishing Group
Absence of a priming effect on dissolved organic carbon degradation in lake water: Absence of priming in lake water
journal, January 2015
- Catalán, Núria; Kellerman, Anne M.; Peter, Hannes
- Limnology and Oceanography, Vol. 60, Issue 1
Respiration in Aquatic Ecosystems
journal, March 2006
- Herndl, G. J.
- Marine Ecology, Vol. 27, Issue 1
The reactivity of plant-derived organic matter and the potential importance of priming effects along the lower Amazon River: ORGANIC MATTER REACTIVITY IN THE AMAZON
journal, June 2016
- Ward, Nicholas D.; Bianchi, Thomas S.; Sawakuchi, Henrique O.
- Journal of Geophysical Research: Biogeosciences, Vol. 121, Issue 6
High rates of daytime respiration in three streams: Use of δ 18 O O2 and O 2 to model diel ecosystem metabolism
journal, April 2014
- Hotchkiss, Erin R.; Hall, Robert O. Jr.
- Limnology and Oceanography, Vol. 59, Issue 3
Comparison of free-living, suspended particle, and aggregate-associated Bacterial and Archaeal communities in the Laptev Sea
journal, September 2009
- Kellogg, Cte; Deming, Jw
- Aquatic Microbial Ecology, Vol. 57
Phylogenetic Analysis of Particle-Attached and Free-Living Bacterial Communities in the Columbia River, Its Estuary, and the Adjacent Coastal Ocean
journal, July 1999
- Crump, Byron C.; Armbrust, E. Virginia; Baross, John A.
- Applied and Environmental Microbiology, Vol. 65, Issue 7
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