Transformation of Recalcitrant Sorghum Varieties Facilitated by Baby Boom and Wuschel2

Nelson-Vasilchik, Kimberly; Hague, Joel; Mookkan, Muruganantham; Zhang, Zhanyuan J.; Kausch, Albert

doi:10.1002/cppb.20076

Title: Transformation of Recalcitrant Sorghum Varieties Facilitated by Baby Boom and Wuschel2

Journal Article · 17 October 2018 · Current Protocols in Plant Biology

DOI: https://doi.org/10.1002/cppb.20076 · OSTI ID:1530823

Nelson-Vasilchik, Kimberly ^[1]; Hague, Joel ^[1]; Mookkan, Muruganantham ^[2]; Zhang, Zhanyuan J. ^[2]; Kausch, Albert ^[1]

Univ. of Rhode Island, Kingston, RI (United States)
Univ. of Missouri, Columbia, MO (United States)

We present that most reliable transformation protocols for cereal crops, including sorghum (Sorghum bicolor L. Moench), rely on the use of immature embryo explants to generate embryogenic callus cells that are then transformed using Agrobacterium- or particle-bombardment-mediated DNA delivery. Subsequent to DNA transfer, most protocols rely on selectable markers for the recovery of stably transformed callus that is then regenerated to produce T₀ plants. However, these protocols require specific genotypes that are innately capable of efficient embryogenic callus initiation. In this study, we describe a system that makes use of the differential expression of the morphogenic regulators Baby Boom (Bbm) and Wuschel2 (Wus2) to achieve transformation in varieties of sorghum typically recalcitrant to standard transformation methods.

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Research Organization:: Donald Danforth Plant Science Center, St. Louis, MO (United States); Univ. of Rhode Island, Kingston, RI (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER)

Grant/Contract Number:: SC0018277

OSTI ID:: 1530823

Alternate ID(s):: OSTI ID: 1479566

Journal Information:: Current Protocols in Plant Biology, Vol. 3, Issue 4; ISSN 2379-8068

Publisher:: WileyCopyright Statement

Country of Publication:: United States

Language:: English

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