Synthesis, lipid membrane incorporation, and ion permeability testing of carbon nanotube porins

Tunuguntla, Ramya H.; Escalada, Artur; A Frolov, Vadim; Noy, Aleksandr

doi:10.1038/nprot.2016.119

Title: Synthesis, lipid membrane incorporation, and ion permeability testing of carbon nanotube porins

Journal Article · 22 September 2016 · Nature Protocols

DOI: https://doi.org/10.1038/nprot.2016.119 · OSTI ID:1530684

Tunuguntla, Ramya H. ^[1]; Escalada, Artur ^[2]; A Frolov, Vadim ^[3]; Noy, Aleksandr ^[4]

Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)
Univ. of Basque Country, Leioa (Spain)
Univ. of Basque Country, Leioa (Spain); Basque Foundation for Science, Bilbao (Spain)
Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Univ. of California Merced, Merced, CA (United States)

Carbon nanotube porins (CNTPs) are 10- to 20-nm-long segments of lipid-stabilized single-walled carbon nanotubes (CNTs) that can be inserted into phospholipid membranes to form nanometer-scale-diameter pores that approximate the geometry and many key transport characteristics of biological membrane channels. We report on protocols for CNTP synthesis by ultrasound-assisted cutting of long CNTs in the presence of lipid amphiphiles, and for validation of CNTP incorporation into a lipid membrane using a proton permeability assay. Moreover, we describe protocols for measuring conductance of individual CNTPs in planar lipid bilayers and plasma membranes of live cells. The protocol for the preparation and testing of the CNTPs in vesicle systems takes 3 d, and single CNTP conductance measurements take 2–5 h. The CNTPs produced by this cutting protocol remain stable and active for at least 10–12 weeks.

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Research Organization:: Lawrence Livermore National Laboratory (LLNL), Livermore, CA (United States)

Sponsoring Organization:: USDOE National Nuclear Security Administration (NNSA); USDOE Office of Science (SC), Basic Energy Sciences (BES) (SC-22). Materials Sciences & Engineering Division; Spanish Ministry of Economy and Competitiveness

Grant/Contract Number:: AC52-07NA27344; AC02-05CH11231

OSTI ID:: 1530684

Report Number(s):: LLNL-JRNL--691260; 819066

Journal Information:: Nature Protocols, Journal Name: Nature Protocols Journal Issue: 10 Vol. 11; ISSN 1754-2189

Publisher:: Nature Publishing GroupCopyright Statement

Country of Publication:: United States

Language:: English

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Carbon Nanotube Porins in Amphiphilic Block Copolymers as Fully Synthetic Mimics of Biological Membranes Sanborn, Jeremy R.; Chen, Xi; Yao, Yun-Chiao Advanced Materials, Vol. 30, Issue 51 https://doi.org/10.1002/adma.201803355	journal	October 2018
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Versatile cyclodextrin nanotube synthesis with functional anchors for efficient ion channel formation: design, characterization and ion conductance Mamad-Hemouch, Hajar; Bacri, Laurent; Huin, Cécile Nanoscale, Vol. 10, Issue 32 https://doi.org/10.1039/c8nr02623h	journal	January 2018
Enhanced water permeability and tunable ion selectivity in subnanometer carbon nanotube porins Tunuguntla, Ramya H.; Henley, Robert Y.; Yao, Yun-Chiao Science, Vol. 357, Issue 6353 https://doi.org/10.1126/science.aan2438	journal	August 2017

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