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Title: Synthesis, lipid membrane incorporation, and ion permeability testing of carbon nanotube porins

Abstract

Carbon nanotube porins (CNTPs) are 10- to 20-nm-long segments of lipid-stabilized single-walled carbon nanotubes (CNTs) that can be inserted into phospholipid membranes to form nanometer-scale-diameter pores that approximate the geometry and many key transport characteristics of biological membrane channels. We report on protocols for CNTP synthesis by ultrasound-assisted cutting of long CNTs in the presence of lipid amphiphiles, and for validation of CNTP incorporation into a lipid membrane using a proton permeability assay. Moreover, we describe protocols for measuring conductance of individual CNTPs in planar lipid bilayers and plasma membranes of live cells. The protocol for the preparation and testing of the CNTPs in vesicle systems takes 3 d, and single CNTP conductance measurements take 2–5 h. The CNTPs produced by this cutting protocol remain stable and active for at least 10–12 weeks.

Authors:
 [1];  [2];  [3];  [4]
  1. Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)
  2. Univ. of Basque Country, Leioa (Spain)
  3. Univ. of Basque Country, Leioa (Spain); Basque Foundation for Science, Bilbao (Spain)
  4. Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Univ. of California Merced, Merced, CA (United States)
Publication Date:
Research Org.:
Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)
Sponsoring Org.:
USDOE National Nuclear Security Administration (NNSA); USDOE Office of Science (SC), Basic Energy Sciences (BES) (SC-22). Materials Sciences & Engineering Division; Spanish Ministry of Economy and Competitiveness
OSTI Identifier:
1530684
Report Number(s):
LLNL-JRNL-691260
Journal ID: ISSN 1754-2189; 819066
Grant/Contract Number:  
AC52-07NA27344; AC02- 05CH11231; BIO2013-49843-EXP
Resource Type:
Accepted Manuscript
Journal Name:
Nature Protocols
Additional Journal Information:
Journal Volume: 11; Journal Issue: 10; Journal ID: ISSN 1754-2189
Publisher:
Nature Publishing Group
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES

Citation Formats

Tunuguntla, Ramya H., Escalada, Artur, A Frolov, Vadim, and Noy, Aleksandr. Synthesis, lipid membrane incorporation, and ion permeability testing of carbon nanotube porins. United States: N. p., 2016. Web. doi:10.1038/nprot.2016.119.
Tunuguntla, Ramya H., Escalada, Artur, A Frolov, Vadim, & Noy, Aleksandr. Synthesis, lipid membrane incorporation, and ion permeability testing of carbon nanotube porins. United States. doi:10.1038/nprot.2016.119.
Tunuguntla, Ramya H., Escalada, Artur, A Frolov, Vadim, and Noy, Aleksandr. Thu . "Synthesis, lipid membrane incorporation, and ion permeability testing of carbon nanotube porins". United States. doi:10.1038/nprot.2016.119. https://www.osti.gov/servlets/purl/1530684.
@article{osti_1530684,
title = {Synthesis, lipid membrane incorporation, and ion permeability testing of carbon nanotube porins},
author = {Tunuguntla, Ramya H. and Escalada, Artur and A Frolov, Vadim and Noy, Aleksandr},
abstractNote = {Carbon nanotube porins (CNTPs) are 10- to 20-nm-long segments of lipid-stabilized single-walled carbon nanotubes (CNTs) that can be inserted into phospholipid membranes to form nanometer-scale-diameter pores that approximate the geometry and many key transport characteristics of biological membrane channels. We report on protocols for CNTP synthesis by ultrasound-assisted cutting of long CNTs in the presence of lipid amphiphiles, and for validation of CNTP incorporation into a lipid membrane using a proton permeability assay. Moreover, we describe protocols for measuring conductance of individual CNTPs in planar lipid bilayers and plasma membranes of live cells. The protocol for the preparation and testing of the CNTPs in vesicle systems takes 3 d, and single CNTP conductance measurements take 2–5 h. The CNTPs produced by this cutting protocol remain stable and active for at least 10–12 weeks.},
doi = {10.1038/nprot.2016.119},
journal = {Nature Protocols},
number = 10,
volume = 11,
place = {United States},
year = {2016},
month = {9}
}

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Cited by: 8 works
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