A biochemical framework for anaerobic oxidation of methane driven by Fe(III)-dependent respiration
Abstract
Consumption of methane by aerobic and anaerobic microbes governs the atmospheric level of this powerful greenhouse gas. Whereas a biochemical understanding of aerobic methanotrophy is well developed, a mechanistic understanding of anaerobic methanotrophy has been prevented by the unavailability of pure cultures. Here we report a biochemical investigation of Methanosarcina acetivorans, a methane-producing species capable of anaerobic methanotrophic growth dependent on reduction of Fe(III). Our findings support a pathway anchored by Fe(III)-dependent mechanisms for energy conservation driving endergonic reactions that are key to methanotrophic growth. The pathway is remarkably similar to pathways hypothesized for uncultured anaerobic methanotrophic archaea. The results contribute to an improved understanding of the methane cycle that is paramount to understanding human interventions influencing Earth’s climate. Finally, the pathway enables advanced development and optimization of biotechnologies converting methane to value-added products through metabolic engineering of M. acetivorans.
- Authors:
-
[2];
- Pennsylvania State Univ., University Park, PA (United States). Dept of Biochemistry and Molecular Biology
- Pennsylvania State Univ., University Park, PA (United States). Dept of Civil and Environmental Engineering
- Publication Date:
- Research Org.:
- Pennsylvania State Univ, University Park, PA (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC), Basic Energy Sciences (BES)
- OSTI Identifier:
- 1529924
- Grant/Contract Number:
- FG02-95ER20198
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Nature Communications
- Additional Journal Information:
- Journal Volume: 9; Journal Issue: 1; Journal ID: ISSN 2041-1723
- Publisher:
- Nature Publishing Group
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 09 BIOMASS FUELS
Citation Formats
Yan, Zhen, Joshi, Prachi, Gorski, Christopher A., and Ferry, James G. A biochemical framework for anaerobic oxidation of methane driven by Fe(III)-dependent respiration. United States: N. p., 2018.
Web. doi:10.1038/s41467-018-04097-9.
Yan, Zhen, Joshi, Prachi, Gorski, Christopher A., & Ferry, James G. A biochemical framework for anaerobic oxidation of methane driven by Fe(III)-dependent respiration. United States. https://doi.org/10.1038/s41467-018-04097-9
Yan, Zhen, Joshi, Prachi, Gorski, Christopher A., and Ferry, James G. Tue .
"A biochemical framework for anaerobic oxidation of methane driven by Fe(III)-dependent respiration". United States. https://doi.org/10.1038/s41467-018-04097-9. https://www.osti.gov/servlets/purl/1529924.
@article{osti_1529924,
title = {A biochemical framework for anaerobic oxidation of methane driven by Fe(III)-dependent respiration},
author = {Yan, Zhen and Joshi, Prachi and Gorski, Christopher A. and Ferry, James G.},
abstractNote = {Consumption of methane by aerobic and anaerobic microbes governs the atmospheric level of this powerful greenhouse gas. Whereas a biochemical understanding of aerobic methanotrophy is well developed, a mechanistic understanding of anaerobic methanotrophy has been prevented by the unavailability of pure cultures. Here we report a biochemical investigation of Methanosarcina acetivorans, a methane-producing species capable of anaerobic methanotrophic growth dependent on reduction of Fe(III). Our findings support a pathway anchored by Fe(III)-dependent mechanisms for energy conservation driving endergonic reactions that are key to methanotrophic growth. The pathway is remarkably similar to pathways hypothesized for uncultured anaerobic methanotrophic archaea. The results contribute to an improved understanding of the methane cycle that is paramount to understanding human interventions influencing Earth’s climate. Finally, the pathway enables advanced development and optimization of biotechnologies converting methane to value-added products through metabolic engineering of M. acetivorans.},
doi = {10.1038/s41467-018-04097-9},
journal = {Nature Communications},
number = 1,
volume = 9,
place = {United States},
year = {2018},
month = {4}
}
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