The Use of Contact Mode Atomic Force Microscopy in Aqueous Medium for Structural Analysis of Spinach Photosynthetic Complexes

Phuthong, Witchukorn; Huang, Zubin; Wittkopp, Tyler M.; Sznee, Kinga; Heinnickel, Mark L.; Dekker, Jan P.; Frese, Raoul N.; Prinz, Fritz B.; Grossman, Arthur R.

doi:10.1104/pp.15.00706

Title: The Use of Contact Mode Atomic Force Microscopy in Aqueous Medium for Structural Analysis of Spinach Photosynthetic Complexes

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Abstract

To investigate the dynamics of photosynthetic pigment-protein complexes in vascular plants at high resolution in an aqueous environment, membrane-protruding oxygen-evolving complexes (OECs) associated with photosystem II (PSII) on spinach (Spinacia oleracea) grana membranes were examined using contact mode atomic force microscopy. This study represents, to our knowledge, the first use of atomic force microscopy to distinguish the putative large extrinsic loop of Photosystem II CP47 reaction center protein (CP47) from the putative oxygen-evolving enhancer proteins 1, 2, and 3 (PsbO, PsbP, and PsbQ) and large extrinsic loop of Photosystem II CP43 reaction center protein (CP43) in the PSII-OEC extrinsic domains of grana membranes under conditions resulting in the disordered arrangement of PSII-OEC particles. Moreover, we observed uncharacterized membrane particles that, based on their physical characteristics and electrophoretic analysis of the polypeptides associated with the grana samples, are hypothesized to be a domain of photosystem I that protrudes from the stromal face of single thylakoid bilayers. Furthermore, our results are interpreted in the context of the results of others that were obtained using cryo-electron microscopy (and single particle analysis), negative staining and freeze-fracture electron microscopy, as well as previous atomic force microscopy studies.

Authors:

^[1]; Huang, Zubin; Wittkopp, Tyler M.; Sznee, Kinga; Heinnickel, Mark L.; Dekker, Jan P.; Frese, Raoul N.; Prinz, Fritz B.; Grossman, Arthur R.

Department of Materials Science and Engineering (W.P., F.B.P.), Department of Mechanical Engineering (Z.H., F.B.P.), and Department of Biology (T.M.W.), Stanford University, Stanford, California 94305,

Publication Date:: Tue Jul 28 00:00:00 EDT 2015

Research Org.:: Energy Frontier Research Centers (EFRC) (United States). Center on Nanostructuring for Efficient Energy Conversion (CNEEC)

Sponsoring Org.:: USDOE Office of Science (SC), Basic Energy Sciences (BES)

OSTI Identifier:: 1529672

Alternate Identifier(s):: OSTI ID: 1386063

Grant/Contract Number:: SC0001060

Resource Type:: Published Article

Journal Name:: Plant Physiology (Bethesda)

Additional Journal Information:: Journal Name: Plant Physiology (Bethesda) Journal Volume: 169 Journal Issue: 2; Journal ID: ISSN 0032-0889

Publisher:: Oxford University Press

Country of Publication:: United States

Language:: English

Subject:: 59 BASIC BIOLOGICAL SCIENCES; catalysis (heterogeneous); solar (fuels); photosynthesis (natural and artificial); bio-inspired; electrodes - solar; defects; charge transport; materials and chemistry by design; synthesis (novel materials)

Citation Formats


                    Phuthong, Witchukorn, Huang, Zubin, Wittkopp, Tyler M., Sznee, Kinga, Heinnickel, Mark L., Dekker, Jan P., Frese, Raoul N., Prinz, Fritz B., and Grossman, Arthur R. The Use of Contact Mode Atomic Force Microscopy in Aqueous Medium for Structural Analysis of Spinach Photosynthetic Complexes.  United States: N. p., 2015. 
Web.  doi:10.1104/pp.15.00706.

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                    Phuthong, Witchukorn, Huang, Zubin, Wittkopp, Tyler M., Sznee, Kinga, Heinnickel, Mark L., Dekker, Jan P., Frese, Raoul N., Prinz, Fritz B., & Grossman, Arthur R. The Use of Contact Mode Atomic Force Microscopy in Aqueous Medium for Structural Analysis of Spinach Photosynthetic Complexes.  United States.  https://doi.org/10.1104/pp.15.00706

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                    Phuthong, Witchukorn, Huang, Zubin, Wittkopp, Tyler M., Sznee, Kinga, Heinnickel, Mark L., Dekker, Jan P., Frese, Raoul N., Prinz, Fritz B., and Grossman, Arthur R. Tue .  
"The Use of Contact Mode Atomic Force Microscopy in Aqueous Medium for Structural Analysis of Spinach Photosynthetic Complexes".  United States.  https://doi.org/10.1104/pp.15.00706.

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@article{osti_1529672,

  title        = {The Use of Contact Mode Atomic Force Microscopy in Aqueous Medium for Structural Analysis of Spinach Photosynthetic Complexes},

  author       = {Phuthong, Witchukorn and Huang, Zubin and Wittkopp, Tyler M. and Sznee, Kinga and Heinnickel, Mark L. and Dekker, Jan P. and Frese, Raoul N. and Prinz, Fritz B. and Grossman, Arthur R.},

  abstractNote = {To investigate the dynamics of photosynthetic pigment-protein complexes in vascular plants at high resolution in an aqueous environment, membrane-protruding oxygen-evolving complexes (OECs) associated with photosystem II (PSII) on spinach (Spinacia oleracea) grana membranes were examined using contact mode atomic force microscopy. This study represents, to our knowledge, the first use of atomic force microscopy to distinguish the putative large extrinsic loop of Photosystem II CP47 reaction center protein (CP47) from the putative oxygen-evolving enhancer proteins 1, 2, and 3 (PsbO, PsbP, and PsbQ) and large extrinsic loop of Photosystem II CP43 reaction center protein (CP43) in the PSII-OEC extrinsic domains of grana membranes under conditions resulting in the disordered arrangement of PSII-OEC particles. Moreover, we observed uncharacterized membrane particles that, based on their physical characteristics and electrophoretic analysis of the polypeptides associated with the grana samples, are hypothesized to be a domain of photosystem I that protrudes from the stromal face of single thylakoid bilayers. Furthermore, our results are interpreted in the context of the results of others that were obtained using cryo-electron microscopy (and single particle analysis), negative staining and freeze-fracture electron microscopy, as well as previous atomic force microscopy studies.},

  doi          = {10.1104/pp.15.00706},

  journal      = {Plant Physiology (Bethesda)},

  number       = 2,

  volume       = 169,

  place        = {United States},

  year         = {Tue Jul 28 00:00:00 EDT 2015},

  month        = {Tue Jul 28 00:00:00 EDT 2015}

}

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