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Title: Radiocarbon Tracers in Toxicology and Medicine: Recent Advances in Technology and Science

Journal Article · · Toxics

This review summarizes recent developments in radiocarbon tracer technology and applications. Technologies covered include accelerator mass spectrometry (AMS), including conversion of samples to graphite, and rapid combustion to carbon dioxide to enable direct liquid sample analysis, coupling to HPLC for real-time AMS analysis, and combined molecular mass spectrometry and AMS for analyte identification and quantitation. Laser-based alternatives, such as cavity ring down spectrometry, are emerging to enable lower cost, higher throughput measurements of biological samples. Applications covered include radiocarbon dating, use of environmental atomic bomb pulse radiocarbon content for cell and protein age determination and turnover studies, and carbon source identification. Low dose toxicology applications reviewed include studies of naphthalene-DNA adduct formation, benzo[a]pyrene pharmacokinetics in humans, and triclocarban exposure and risk assessment. Cancer-related studies covered include the use of radiocarbon-labeled cells for better defining mechanisms of metastasis and the use of drug-DNA adducts as predictive biomarkers of response to chemotherapy

Research Organization:
Lawrence Livermore National Laboratory (LLNL), Livermore, CA (United States)
Sponsoring Organization:
USDOE National Nuclear Security Administration (NNSA)
Grant/Contract Number:
AC52-07NA27344; LDRD 08-LW-100
OSTI ID:
1529655
Alternate ID(s):
OSTI ID: 1559419
Report Number(s):
LLNL-JRNL-768802; TOXIC8; 959844
Journal Information:
Toxics, Vol. 7, Issue 2; ISSN 2305-6304
Publisher:
MDPICopyright Statement
Country of Publication:
United States
Language:
English
Citation Metrics:
Cited by: 7 works
Citation information provided by
Web of Science

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A microdosing approach for characterizing formation and repair of carboplatin-DNA monoadducts and chemoresistance journal March 2011
A diagnostic microdosing approach to investigate platinum sensitivity in non-small cell lung cancer: Diagnostic microdosing approach journal May 2017
CRIMS: Chemical reaction interface mass spectrometry journal July 1994
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Towards biomarker-dependent individualized chemotherapy: Exploring cell-specific differences in oxaliplatin–DNA adduct distribution using accelerator mass spectrometry journal April 2010
Dynamics of Hippocampal Neurogenesis in Adult Humans journal June 2013
The impact of early human data on clinical development: there is time to win journal June 2016
Profiling of dalcetrapib metabolites in human plasma by accelerator mass spectrometry and investigation of the free phenothiol by derivatisation with methylacrylate journal April 2018
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Critical Role of Histone Turnover in Neuronal Transcription and Plasticity journal July 2015
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  • Nuclear Instruments and Methods in Physics Research Section B: Beam Interactions with Materials and Atoms, Vol. 259, Issue 1 https://doi.org/10.1016/j.nimb.2007.01.215
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Free flow electrophoresis separation and AMS quantitation of 14C–naphthalene–protein adducts
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  • Nuclear Instruments and Methods in Physics Research Section B: Beam Interactions with Materials and Atoms, Vol. 268, Issue 7-8 https://doi.org/10.1016/j.nimb.2009.10.164
journal April 2010
Installation of hybrid ion source on the 1-MV LLNL BioAMS spectrometer journal January 2013
An interface for the direct coupling of small liquid samples to AMS journal October 2015
Naphthalene DNA adduct formation and tolerance in the lung
  • Buchholz, Bruce A.; Carratt, Sarah A.; Kuhn, Edward A.
  • Nuclear Instruments and Methods in Physics Research Section B: Beam Interactions with Materials and Atoms, Vol. 438 https://doi.org/10.1016/j.nimb.2018.07.004
journal January 2019
Nasal dosimetry of inspired naphthalene vapor in the male and female B6C3F1 mouse journal July 2013
A review of whole animal bioassays of the carcinogenic potential of naphthalene journal July 2008
Accelerator Mass Spectrometry journal June 1995
Dating Techniques in Archaeology and Paleoanthropology journal February 1987
A High-Throughput Method for the Conversion of CO 2 Obtained from Biochemical Samples to Graphite in Septa-Sealed Vials for Quantification of 14 C via Accelerator Mass Spectrometry journal May 2003
Elemental Speciation by Parallel Elemental and Molecular Mass Spectrometry and Peak Profile Matching journal December 2006
Automated Combustion Accelerator Mass Spectrometry for the Analysis of Biomedical Samples in the Low Attomole Range journal July 2014
Intracavity Optogalvanic Spectroscopy. An Analytical Technique for 14 C Analysis with Subattomole Sensitivity journal July 2008
Quantifying Carbon-14 for Biology Using Cavity Ring-Down Spectroscopy journal August 2016
Paclitaxel Enhances Carboplatin-DNA Adduct Formation and Cytotoxicity journal November 2015
Human Microdosing with Carcinogenic Polycyclic Aromatic Hydrocarbons: In Vivo Pharmacokinetics of Dibenzo[ def,p ]chrysene and Metabolites by UPLC Accelerator Mass Spectrometry journal September 2016
DNA Adducts from Anticancer Drugs as Candidate Predictive Markers for Precision Medicine journal December 2016
Toward Predicting Acute Myeloid Leukemia Patient Response to 7 + 3 Induction Chemotherapy via Diagnostic Microdosing journal August 2018
Optical Measurement of Radiocarbon below Unity Fraction Modern by Linear Absorption Spectroscopy journal September 2017
Gemcitabine Causes Minimal Modulation of Carboplatin-DNA Monoadduct Formation and Repair in Bladder Cancer Cells journal November 2010
Characterization of Oxaliplatin−DNA Adduct Formation in DNA and Differentiation of Cancer Cell Drug Sensitivity at Microdose Concentrations journal December 2007
Examination by laser scanning confocal fluorescence imaging microscopy of the subcellular localisation of anthracyclines in parent and multidrug resistant cell lines journal June 1993
Systems biology of cisplatin resistance: past, present and future journal May 2014
The resurgence of platinum-based cancer chemotherapy journal July 2007
Cellular processing of platinum anticancer drugs journal March 2005
Tracking Tumor Colonization in Xenograft Mouse Models Using Accelerator Mass Spectrometry journal October 2018
The status of platinum anticancer drugs in the clinic and in clinical trials journal January 2010
Neocortical neurogenesis in humans is restricted to development journal August 2006
Lethality of human myeloblasts correlates with the incorporation of arabinofuranosylcytosine into DNA. journal May 1981
The v 3 band of 14 C 16 O 2 molecule measured by optical-frequency-comb-assisted cavity ring-down spectroscopy journal September 2011
Absorption, distribution, metabolism and excretion (ADME) of the ALK inhibitor alectinib: results from an absolute bioavailability and mass balance study in healthy subjects journal May 2016
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Lack of tissue renewal in human adult Achilles tendon is revealed by nuclear bomb 14 C journal May 2013
A Decade in the MIST: Learnings from Investigations of Drug Metabolites in Drug Development under the “Metabolites in Safety Testing” Regulatory Guidance journal February 2018
Evidence for Cardiomyocyte Renewal in Humans journal April 2009
Radiocarbon dating reveals minimal collagen turnover in both healthy and osteoarthritic human cartilage journal July 2016
Age and acute myeloid leukemia: real world data on decision to treat and outcomes from the Swedish Acute Leukemia Registry journal April 2009
A randomized study of high-dose cytarabine in induction in acute myeloid leukemia [see comments] journal March 1996
Significant Human β-Cell Turnover Is Limited to the First Three Decades of Life as Determined by in Vivo Thymidine Analog Incorporation and Radiocarbon Dating journal October 2010
Prognostic Impact of Gastroduodenal Artery Involvement in Cancer of the Pancreatic Head journal November 2022
Radiocarbon Dating of the Human Eye Lens Crystallines Reveal Proteins without Carbon Turnover throughout Life journal January 2008
Carotid Plaque Age Is a Feature of Plaque Stability Inversely Related to Levels of Plasma Insulin journal April 2011
Molecular Dissection of Induced Platinum Resistance through Functional and Gene Expression Analysis in a Cell Culture Model of Bladder Cancer journal January 2016
Maternal exposure to an environmentally relevant dose of triclocarban results in perinatal exposure and potential alterations in offspring development in the mouse model journal August 2017
Nanotracing and cavity-ring down spectroscopy: A new ultrasensitive approach in large molecule drug disposition studies journal October 2018
Bioanalytical Applications of Accelerator Mass Spectrometry for Pharmaceutical Research journal July 2000
Naphthalene: A Respiratory Tract Toxicant and Carcinogen for Mice journal January 1992
Radiocarbon Dioxide detection based on Cavity Ring-Down Spectroscopy and a Quantum Cascade Laser text January 2015