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Title: Engineering microbial consortia by division of labor

Abstract

During microbial applications, metabolic burdens can lead to a signifcant drop in cell performance. Novel synthetic biology tools or multi-step bioprocessing (e.g., fermentation followed by chemical conversions) are therefore needed to avoid compromised biochemical productivity from over-burdened cells. A possible solution to address metabolic burden is Division of Labor (DoL) via natural and synthetic microbial consortia. In particular, consolidated bioprocesses and metabolic cooperation for detoxifcation or cross feeding (e.g., vitamin C fermentation) have shown numerous successes in industrial level applications. However, distributing a metabolic pathway among proper hosts remains an engineering conundrum due to several challenges: complex subpopulation dynamics/interactions with a short time-window for stable production, suboptimal cultivation of microbial communities, proliferation of cheaters or low-producers, intermediate metabolite dilution, transport barriers between species, and breaks in metabolite channeling through biosynthesis pathways. To develop stable consortia, optimization of strain inoculations, nutritional divergence and crossing feeding, evolution of mutualistic growth, cell immobilization, and biosensors may potentially be used to control cell populations. Another opportunity is direct integration of non-bioprocesses (e.g., microbial electrosynthesis) to power cell metabolism and improve carbon efciency. Additionally, metabolic modeling and 13C-metabolic fux analysis of mixed culture metabolism and cross-feeding ofers a computational approach to complement experimental research formore » improved consortia performance.« less

Authors:
 [1];  [2];  [1];  [2];  [3]; ORCiD logo [1]
  1. Washington Univ., St. Louis, MO (United States)
  2. Rensselaer Polytechnic Inst., Troy, NY (United States)
  3. Virginia Commonwealth Univ., Richmond, VA (United States)
Publication Date:
Research Org.:
Washington Univ., St. Louis, MO (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Biological and Environmental Research (BER) (SC-23)
Contributing Org.:
Rensselaer Polytechnic Institute, Virginia Commonwealth University
OSTI Identifier:
1529583
Grant/Contract Number:  
SC0018324
Resource Type:
Accepted Manuscript
Journal Name:
Microbial Cell Factories
Additional Journal Information:
Journal Volume: 18; Journal Issue: 1; Journal ID: ISSN 1475-2859
Publisher:
BioMed Central
Country of Publication:
United States
Language:
English
Subject:
09 BIOMASS FUELS; 59 BASIC BIOLOGICAL SCIENCES; 13C-metabolic fux analysis; Cross-feeding; Metabolite channeling; Reporter protein; Subpopulations

Citation Formats

Roell, Garrett W., Zha, Jian, Carr, Rhiannon R., Koffas, Mattheos A., Fong, Stephen S., and Tang, Yinjie J. Engineering microbial consortia by division of labor. United States: N. p., 2019. Web. doi:10.1186/s12934-019-1083-3.
Roell, Garrett W., Zha, Jian, Carr, Rhiannon R., Koffas, Mattheos A., Fong, Stephen S., & Tang, Yinjie J. Engineering microbial consortia by division of labor. United States. doi:10.1186/s12934-019-1083-3.
Roell, Garrett W., Zha, Jian, Carr, Rhiannon R., Koffas, Mattheos A., Fong, Stephen S., and Tang, Yinjie J. Fri . "Engineering microbial consortia by division of labor". United States. doi:10.1186/s12934-019-1083-3. https://www.osti.gov/servlets/purl/1529583.
@article{osti_1529583,
title = {Engineering microbial consortia by division of labor},
author = {Roell, Garrett W. and Zha, Jian and Carr, Rhiannon R. and Koffas, Mattheos A. and Fong, Stephen S. and Tang, Yinjie J.},
abstractNote = {During microbial applications, metabolic burdens can lead to a signifcant drop in cell performance. Novel synthetic biology tools or multi-step bioprocessing (e.g., fermentation followed by chemical conversions) are therefore needed to avoid compromised biochemical productivity from over-burdened cells. A possible solution to address metabolic burden is Division of Labor (DoL) via natural and synthetic microbial consortia. In particular, consolidated bioprocesses and metabolic cooperation for detoxifcation or cross feeding (e.g., vitamin C fermentation) have shown numerous successes in industrial level applications. However, distributing a metabolic pathway among proper hosts remains an engineering conundrum due to several challenges: complex subpopulation dynamics/interactions with a short time-window for stable production, suboptimal cultivation of microbial communities, proliferation of cheaters or low-producers, intermediate metabolite dilution, transport barriers between species, and breaks in metabolite channeling through biosynthesis pathways. To develop stable consortia, optimization of strain inoculations, nutritional divergence and crossing feeding, evolution of mutualistic growth, cell immobilization, and biosensors may potentially be used to control cell populations. Another opportunity is direct integration of non-bioprocesses (e.g., microbial electrosynthesis) to power cell metabolism and improve carbon efciency. Additionally, metabolic modeling and 13C-metabolic fux analysis of mixed culture metabolism and cross-feeding ofers a computational approach to complement experimental research for improved consortia performance.},
doi = {10.1186/s12934-019-1083-3},
journal = {Microbial Cell Factories},
number = 1,
volume = 18,
place = {United States},
year = {2019},
month = {2}
}

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Figures / Tables:

Figure 1 Figure 1: Overview of cellular processes, metabolic burdens, and resource allocations in engineered microbial hosts (left) and the consortia maintenance (right)

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    Figures/Tables have been extracted from DOE-funded journal article accepted manuscripts.