Draft Genome Sequence of Cyanobacterium sp. Strain HL-69, Isolated from a Benthic Microbial Mat from a Magnesium Sulfate-Dominated Hypersaline Lake

Mobberley, J. M.; Romine, M. F.; Cole, J. K.; Maezato, Y.; Lindemann, S. R.; Nelson, W. C.

doi:10.1128/genomeA.01583-17

Title: Draft Genome Sequence of Cyanobacterium sp. Strain HL-69, Isolated from a Benthic Microbial Mat from a Magnesium Sulfate-Dominated Hypersaline Lake

Abstract

ABSTRACT The complete genome sequence of Cyanobacterium sp. strain HL-69 consists of 3,155,247 bp and contains 2,897 predicted genes comprising a chromosome and two plasmids. The genome is consistent with a halophilic nondiazotrophic phototrophic lifestyle, and this organism is able to synthesize most B vitamins and produces several secondary metabolites.

Authors:

^[1]; Romine, M. F. ^[1]; Cole, J. K. ^[1]; Maezato, Y. ^[1]; Lindemann, S. R. ^[1];

^[1]

Biological Sciences Division, Pacific Northwest National Laboratory, Richland, Washington, USA

Publication Date:: Thu Feb 08 00:00:00 EST 2018

Research Org.:: Pacific Northwest National Lab. (PNNL), Richland, WA (United States)

Sponsoring Org.:: USDOE

OSTI Identifier:: 1530920

Alternate Identifier(s):: OSTI ID: 1528963

Report Number(s):: PNNL-SA-129362
Journal ID: ISSN 2169-8287; e01583-17

Grant/Contract Number:: AC02-05CH11231; AC05-76RL01830

Resource Type:: Published Article

Journal Name:: Genome Announcements

Additional Journal Information:: Journal Name: Genome Announcements Journal Volume: 6 Journal Issue: 6; Journal ID: ISSN 2169-8287

Publisher:: American Society for Microbiology

Country of Publication:: United States

Language:: English

Subject:: 59 BASIC BIOLOGICAL SCIENCES; hypersaline; cyanobacteria; complete genome

Citation Formats


                    Mobberley, J. M., Romine, M. F., Cole, J. K., Maezato, Y., Lindemann, S. R., and Nelson, W. C. Draft Genome Sequence of Cyanobacterium sp. Strain HL-69, Isolated from a Benthic Microbial Mat from a Magnesium Sulfate-Dominated Hypersaline Lake.  United States: N. p., 2018. 
Web.  doi:10.1128/genomeA.01583-17.

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                    Mobberley, J. M., Romine, M. F., Cole, J. K., Maezato, Y., Lindemann, S. R., & Nelson, W. C. Draft Genome Sequence of Cyanobacterium sp. Strain HL-69, Isolated from a Benthic Microbial Mat from a Magnesium Sulfate-Dominated Hypersaline Lake.  United States.  https://doi.org/10.1128/genomeA.01583-17

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                    Mobberley, J. M., Romine, M. F., Cole, J. K., Maezato, Y., Lindemann, S. R., and Nelson, W. C. Thu .  
"Draft Genome Sequence of Cyanobacterium sp. Strain HL-69, Isolated from a Benthic Microbial Mat from a Magnesium Sulfate-Dominated Hypersaline Lake".  United States.  https://doi.org/10.1128/genomeA.01583-17.

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@article{osti_1530920,

  title        = {Draft Genome Sequence of Cyanobacterium sp. Strain HL-69, Isolated from a Benthic Microbial Mat from a Magnesium Sulfate-Dominated Hypersaline Lake},

  author       = {Mobberley, J. M. and Romine, M. F. and Cole, J. K. and Maezato, Y. and Lindemann, S. R. and Nelson, W. C.},

  abstractNote = {ABSTRACT The complete genome sequence of Cyanobacterium sp. strain HL-69 consists of 3,155,247 bp and contains 2,897 predicted genes comprising a chromosome and two plasmids. The genome is consistent with a halophilic nondiazotrophic phototrophic lifestyle, and this organism is able to synthesize most B vitamins and produces several secondary metabolites.},

  doi          = {10.1128/genomeA.01583-17},

  journal      = {Genome Announcements},

  number       = 6,

  volume       = 6,

  place        = {United States},

  year         = {Thu Feb 08 00:00:00 EST 2018},

  month        = {Thu Feb 08 00:00:00 EST 2018}

}

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Works referenced in this record:

Geo- and biogeochemical processes in a heliothermal hypersaline lake
journal, May 2016

Zachara, John M.; Moran, James J.; Resch, Charles T.
Geochimica et Cosmochimica Acta, Vol. 181
DOI: 10.1016/j.gca.2016.02.001

Rfam 12.0: updates to the RNA families database
journal, November 2014

Nawrocki, Eric P.; Burge, Sarah W.; Bateman, Alex
Nucleic Acids Research, Vol. 43, Issue D1
DOI: 10.1093/nar/gku1063

BlastKOALA and GhostKOALA: KEGG Tools for Functional Characterization of Genome and Metagenome Sequences
journal, February 2016

Kanehisa, Minoru; Sato, Yoko; Morishima, Kanae
Journal of Molecular Biology, Vol. 428, Issue 4
DOI: 10.1016/j.jmb.2015.11.006

The TIGRFAMs database of protein families
journal, January 2003

Haft, D. H.
Nucleic Acids Research, Vol. 31, Issue 1
DOI: 10.1093/nar/gkg128

Molecular Ecology of Hypersaline Microbial Mats: Current Insights and New Directions
journal, January 2016

Wong, Hon; Ahmed-Cox, Aria; Burns, Brendan
Microorganisms, Vol. 4, Issue 1
DOI: 10.3390/microorganisms4010006

Prodigal: prokaryotic gene recognition and translation initiation site identification
journal, March 2010

Hyatt, Doug; Chen, Gwo-Liang; LoCascio, Philip F.
BMC Bioinformatics, Vol. 11, Issue 1
DOI: 10.1186/1471-2105-11-119

Organismal and spatial partitioning of energy and macronutrient transformations within a hypersaline mat
journal, March 2017

Mobberley, Jennifer M.; Lindemann, Stephen R.; Bernstein, Hans C.
FEMS Microbiology Ecology, Vol. 93, Issue 4
DOI: 10.1093/femsec/fix028

The epsomitic phototrophic microbial mat of Hot Lake, Washington: community structural responses to seasonal cycling
journal, January 2013

Lindemann, Stephen R.; Moran, James J.; Stegen, James C.
Frontiers in Microbiology, Vol. 4
DOI: 10.3389/fmicb.2013.00323

Draft Genome Sequence of Cyanobacterium sp. Strain IPPAS B-1200 with a Unique Fatty Acid Composition
journal, November 2016

Starikov, Alexander Y.; Usserbaeva, Aizhan A.; Sinetova, Maria A.
Genome Announcements, Vol. 4, Issue 6
DOI: 10.1128/genomeA.01306-16

Underlying mechanisms for syntrophic metabolism of essential enzyme cofactors in microbial communities
journal, February 2017

Romine, Margaret F.; Rodionov, Dmitry A.; Maezato, Yukari
The ISME Journal, Vol. 11, Issue 6
DOI: 10.1038/ismej.2017.2

Multiple Roles of Photosynthetic and Sunscreen Pigments in Cyanobacteria Focusing on the Oxidative Stress
journal, May 2013

Wada, Naoki; Sakamoto, Toshio; Matsugo, Seiichi
Metabolites, Vol. 3, Issue 2
DOI: 10.3390/metabo3020463

Nonhybrid, finished microbial genome assemblies from long-read SMRT sequencing data
journal, May 2013

Chin, Chen-Shan; Alexander, David H.; Marks, Patrick
Nature Methods, Vol. 10, Issue 6
DOI: 10.1038/nmeth.2474

Generic Assignments, Strain Histories and Properties of Pure Cultures of Cyanobacteria
journal, March 1979

Stanier, Roger Y.; Deruelles, Josette; Rippka, Rosmarie
Microbiology, Vol. 111, Issue 1, p. 1-61
DOI: 10.1099/00221287-111-1-1

Phototrophic biofilm assembly in microbial-mat-derived unicyanobacterial consortia: model systems for the study of autotroph-heterotroph interactions
journal, April 2014

Cole, Jessica K.; Hutchison, Janine R.; Renslow, Ryan S.
Frontiers in Microbiology, Vol. 5
DOI: 10.3389/fmicb.2014.00109

The RAST Server: Rapid Annotations using Subsystems Technology
journal, January 2008

Aziz, Ramy K.; Bartels, Daniela; Best, Aaron A.
BMC Genomics, Vol. 9, Issue 1, Article No. 75
DOI: 10.1186/1471-2164-9-75

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Title: Draft Genome Sequence of Cyanobacterium sp. Strain HL-69, Isolated from a Benthic Microbial Mat from a Magnesium Sulfate-Dominated Hypersaline Lake

Abstract

Citation Formats

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Prodigal: prokaryotic gene recognition and translation initiation site identification
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