Crystal structure of the Redβ C-terminal domain in complex with λ Exonuclease reveals an unexpected homology with λ Orf and an interaction with Escherichia coli single stranded DNA binding protein

Caldwell, Brian J.; Zakharova, Ekaterina; Filsinger, Gabriel T.; Wannier, Timothy M.; Hempfling, Jordan P.; Chun-Der, Lee; Pei, Dehua; Church, George M.; Bell, Charles E.

doi:10.1093/nar/gky1309

Title: Crystal structure of the Redβ C-terminal domain in complex with λ Exonuclease reveals an unexpected homology with λ Orf and an interaction with Escherichia coli single stranded DNA binding protein

Journal Article · 09 January 2019 · Nucleic Acids Research

DOI: https://doi.org/10.1093/nar/gky1309 · OSTI ID:1528913

Caldwell, Brian J. ^[1]; Zakharova, Ekaterina ^[1]; Filsinger, Gabriel T. ^[2]; Wannier, Timothy M. ^[2]; Hempfling, Jordan P. ^[1]; Chun-Der, Lee ^[1]; Pei, Dehua ^[1]; Church, George M. ^[3]; Bell, Charles E. ^[1]

The Ohio State Univ., Columbus, OH (United States)
Harvard Medical School, Cambridge, MA (United States)
Harvard Medical School, Cambridge, MA (United States); Harvard Univ., Cambridge, MA (United States)

Bacteriophage λ encodes a DNA recombination system that includes a 5'-3' exonuclease (λ Exo) and a single strand annealing protein (Redβ). The two proteins form a complex that is thought to mediate loading of Redβ directly onto the single-stranded 3'-overhang generated by λ Exo. Here, we present a 2.3 Å crystal structure of the λ Exo trimer bound to three copies of the Redβ C-terminal domain (CTD). Mutation of residues at the hydrophobic core of the interface disrupts complex formation in vitro and impairs recombination in vivo. The Redβ CTD forms a three-helix bundle with unexpected structural homology to phage λ Orf, a protein that binds to E. coli single-stranded DNA binding protein (SSB) to function as a recombination mediator. Based on this relationship, we found that Redβ binds to full-length SSB, and to a peptide corresponding to its nine C-terminal residues, in an interaction that requires the CTD. These results suggest a dual role of the CTD, first in binding to λ Exo to facilitate loading of Redβ directly onto the initial single-stranded DNA (ssDNA) at a 3'-overhang, and second in binding to SSB to facilitate annealing of the overhang to SSB-coated ssDNA at the replication fork.

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Research Organization:: Harvard Medical School, Cambridge, MA (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER). Biological Systems Science Division

Grant/Contract Number:: FG02-02ER63445

OSTI ID:: 1528913

Journal Information:: Nucleic Acids Research, Vol. 47, Issue 4; ISSN 0305-1048

Publisher:: Oxford University PressCopyright Statement

Country of Publication:: United States

Language:: English

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Cited by: 15 works

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59 BASIC BIOLOGICAL SCIENCES
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Title: Crystal structure of the Redβ C-terminal domain in complex with λ Exonuclease reveals an unexpected homology with λ Orf and an interaction with Escherichia coli single stranded DNA binding protein

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