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Title: A nanochannel through a plasmonic antenna gap: an integrated device for single particle counting

Abstract

Plasmonic nanoantennas are ideal for single molecule detection since they nano-focus the light beyond diffraction and enhance the optical fields by several orders of magnitude. But delivering the molecules into these nanometric hot-spots is a real challenge. Here, we present a dynamic sensor, with label-free real-time detection capabilities, which can detect and count molecules and particles one by one in their native environment independently of their concentration. To this end, we have integrated a 35 nm gap plasmonic bowtie antenna with a 30 nm × 30 nm nanochannel. The channel runs through the antenna gap, and delivers the analyte directly into the hot spot. We show how the antenna probes into zeptoliter volumes inside the nanochannel by observing the dark field resonance shift during the filling process of a non-fluorescent liquid. Moreover, we detect and count single quantum dots, one by one, at ultra-high concentrations of up to 25 mg mL-1. The nano-focusing of light, reduces the observation volume in five orders of magnitude compared to the diffraction limited spot, beating the diffraction limit. These results prove the unique sensitivity of the device and in the future can be extended to detection of a variety of molecules for biomedical applications.

Authors:
ORCiD logo [1];  [2];  [2];  [2];  [2]
  1. Molecular Foundry, Lawrence Berkeley National Laboratory, USA, DTU Nanotech, Technical University of Denmark
  2. Molecular Foundry, Lawrence Berkeley National Laboratory, USA
Publication Date:
Research Org.:
Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Basic Energy Sciences (BES) (SC-22)
OSTI Identifier:
1526649
Alternate Identifier(s):
OSTI ID: 1596678
Grant/Contract Number:  
AC02-05CH11231
Resource Type:
Published Article
Journal Name:
Lab on a chip (Print)
Additional Journal Information:
Journal Name: Lab on a chip (Print) Journal Volume: 19 Journal Issue: 14; Journal ID: ISSN 1473-0197
Publisher:
Royal Society of Chemistry
Country of Publication:
France
Language:
English
Subject:
77 NANOSCIENCE AND NANOTECHNOLOGY

Citation Formats

Fernandez-Cuesta, Irene, West, Melanie Maputol, Montinaro, Enrica, Schwartzberg, Adam, and Cabrini, Stefano. A nanochannel through a plasmonic antenna gap: an integrated device for single particle counting. France: N. p., 2019. Web. doi:10.1039/C9LC00186G.
Fernandez-Cuesta, Irene, West, Melanie Maputol, Montinaro, Enrica, Schwartzberg, Adam, & Cabrini, Stefano. A nanochannel through a plasmonic antenna gap: an integrated device for single particle counting. France. doi:10.1039/C9LC00186G.
Fernandez-Cuesta, Irene, West, Melanie Maputol, Montinaro, Enrica, Schwartzberg, Adam, and Cabrini, Stefano. Tue . "A nanochannel through a plasmonic antenna gap: an integrated device for single particle counting". France. doi:10.1039/C9LC00186G.
@article{osti_1526649,
title = {A nanochannel through a plasmonic antenna gap: an integrated device for single particle counting},
author = {Fernandez-Cuesta, Irene and West, Melanie Maputol and Montinaro, Enrica and Schwartzberg, Adam and Cabrini, Stefano},
abstractNote = {Plasmonic nanoantennas are ideal for single molecule detection since they nano-focus the light beyond diffraction and enhance the optical fields by several orders of magnitude. But delivering the molecules into these nanometric hot-spots is a real challenge. Here, we present a dynamic sensor, with label-free real-time detection capabilities, which can detect and count molecules and particles one by one in their native environment independently of their concentration. To this end, we have integrated a 35 nm gap plasmonic bowtie antenna with a 30 nm × 30 nm nanochannel. The channel runs through the antenna gap, and delivers the analyte directly into the hot spot. We show how the antenna probes into zeptoliter volumes inside the nanochannel by observing the dark field resonance shift during the filling process of a non-fluorescent liquid. Moreover, we detect and count single quantum dots, one by one, at ultra-high concentrations of up to 25 mg mL-1. The nano-focusing of light, reduces the observation volume in five orders of magnitude compared to the diffraction limited spot, beating the diffraction limit. These results prove the unique sensitivity of the device and in the future can be extended to detection of a variety of molecules for biomedical applications.},
doi = {10.1039/C9LC00186G},
journal = {Lab on a chip (Print)},
number = 14,
volume = 19,
place = {France},
year = {2019},
month = {7}
}

Journal Article:
Free Publicly Available Full Text
Publisher's Version of Record
DOI: 10.1039/C9LC00186G

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