Simple measurements in a complex system: soil community responses to nitrogen amendment in a Pinus taeda forest
Abstract
Abstract Microorganisms regulate the decomposition of soil organic matter and the flow of plant‐available nutrients. In a temperate pine forest in North Carolina, USA , where nitrogen (N) had been experimentally added for eight years, we combined DNA ‐based measures of fungal and bacterial biomass and composition with measures of seven extracellular enzymes (ecoenzymes). These measures were then correlated with soil chemistry. Our goals were to evaluate the relative sensitivity and repeatability of multiple structural and functional measures of community organization to long‐term N deposition. Measurements were conducted at three litter/soil depths, corresponding to the litter, Oa, and mineral A horizons (max depth 10 cm) with ten spatial replicates per experimental plot. Soil chemistry differed significantly with soil depth and N amendment. Total biomass (soil DNA ), as well as fungal and bacterial biomass (measured by qPCR ), was greatest in the litter horizon and declined significantly with depth. Ecoenzyme activity patterns also changed with soil depth, transitioning from high levels of C, N, and P metabolizing activities in the litter horizon to increased oxidative activities at the lower depth. Under N amendment, soil pH decreased and nitrate concentrations increased in all three soil horizons. Correspondingly, the estimated microbial C usemore »
- Authors:
-
[1];
- Bioscience Division Los Alamos National Laboratory M888 Los Alamos New Mexico USA
- Biology Department University of New Mexico Albuquerque New Mexico USA
- Publication Date:
- Research Org.:
- Los Alamos National Laboratory (LANL), Los Alamos, NM (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC), Biological and Environmental Research (BER)
- OSTI Identifier:
- 1505642
- Alternate Identifier(s):
- OSTI ID: 1505643; OSTI ID: 1525839
- Report Number(s):
- LA-UR-18-23932
Journal ID: ISSN 2150-8925; e02687
- Grant/Contract Number:
- 89233218CNA000001
- Resource Type:
- Published Article
- Journal Name:
- Ecosphere
- Additional Journal Information:
- Journal Name: Ecosphere Journal Volume: 10 Journal Issue: 4; Journal ID: ISSN 2150-8925
- Publisher:
- Wiley Blackwell (John Wiley & Sons)
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 54 ENVIRONMENTAL SCIENCES; Biological Science; carbon use efficiency; extracellular ecoenzymes; N amendment; ribosomal RNA genes; soil bacterial community; soil carbon cycling; soil fungal community; soil microbiome; temperate pine forest
Citation Formats
Kuske, Cheryl R., Sinsabaugh, Robert L., Gallegos‐Graves, La Verne, Albright, Michaeline B. N., Mueller, Rebecca, and Dunbar, John. Simple measurements in a complex system: soil community responses to nitrogen amendment in a Pinus taeda forest. United States: N. p., 2019.
Web. doi:10.1002/ecs2.2687.
Kuske, Cheryl R., Sinsabaugh, Robert L., Gallegos‐Graves, La Verne, Albright, Michaeline B. N., Mueller, Rebecca, & Dunbar, John. Simple measurements in a complex system: soil community responses to nitrogen amendment in a Pinus taeda forest. United States. https://doi.org/10.1002/ecs2.2687
Kuske, Cheryl R., Sinsabaugh, Robert L., Gallegos‐Graves, La Verne, Albright, Michaeline B. N., Mueller, Rebecca, and Dunbar, John. Fri .
"Simple measurements in a complex system: soil community responses to nitrogen amendment in a Pinus taeda forest". United States. https://doi.org/10.1002/ecs2.2687.
@article{osti_1505642,
title = {Simple measurements in a complex system: soil community responses to nitrogen amendment in a Pinus taeda forest},
author = {Kuske, Cheryl R. and Sinsabaugh, Robert L. and Gallegos‐Graves, La Verne and Albright, Michaeline B. N. and Mueller, Rebecca and Dunbar, John},
abstractNote = {Abstract Microorganisms regulate the decomposition of soil organic matter and the flow of plant‐available nutrients. In a temperate pine forest in North Carolina, USA , where nitrogen (N) had been experimentally added for eight years, we combined DNA ‐based measures of fungal and bacterial biomass and composition with measures of seven extracellular enzymes (ecoenzymes). These measures were then correlated with soil chemistry. Our goals were to evaluate the relative sensitivity and repeatability of multiple structural and functional measures of community organization to long‐term N deposition. Measurements were conducted at three litter/soil depths, corresponding to the litter, Oa, and mineral A horizons (max depth 10 cm) with ten spatial replicates per experimental plot. Soil chemistry differed significantly with soil depth and N amendment. Total biomass (soil DNA ), as well as fungal and bacterial biomass (measured by qPCR ), was greatest in the litter horizon and declined significantly with depth. Ecoenzyme activity patterns also changed with soil depth, transitioning from high levels of C, N, and P metabolizing activities in the litter horizon to increased oxidative activities at the lower depth. Under N amendment, soil pH decreased and nitrate concentrations increased in all three soil horizons. Correspondingly, the estimated microbial C use efficiency decreased in N‐amended soils at all depths, despite differences in microbial biomass, community composition, and soil chemistry. Overall, bacterial composition was most responsive to nitrogen amendment, but the taxonomic context of the response varied with soil horizons in conjunction with shifts in soil chemistry and enzyme activities. While a single measure that would incorporate all of the C, N, and P metabolizing activities did not emerge, many measures correlated with each other, and/or with depth and/or N amendment.},
doi = {10.1002/ecs2.2687},
journal = {Ecosphere},
number = 4,
volume = 10,
place = {United States},
year = {Fri Apr 05 00:00:00 EDT 2019},
month = {Fri Apr 05 00:00:00 EDT 2019}
}
Free Publicly Available Full Text
Publisher's Version of Record
https://doi.org/10.1002/ecs2.2687
https://doi.org/10.1002/ecs2.2687
Other availability
Search WorldCat to find libraries that may hold this journalFigures / Tables:
Fig. 1: Profile of soil core collected from the pine Duke Forest free air CO2 enrichment site. Samples collected here were the litter/organic, the 0–2 cm Oa, and the 5–10 cm A horizons. The 2–5 cm upper A horizon was not analyzed due to heterogeneity.
All figures and tables
(8 total)
Save to My Library
You must Sign In or Create an Account in order to save documents to your library.
Works referenced in this record:
PROGRESSIVE NITROGEN LIMITATION OF ECOSYSTEM PROCESSES UNDER ELEVATED CO 2 IN A WARM-TEMPERATE FOREST
journal, January 2006
- Finzi, Adrien C.; Moore, David J. P.; DeLucia, Evan H.
- Ecology, Vol. 87, Issue 1
Model formulation of microbial CO2 production and efficiency can significantly influence short and long term soil C projections
journal, March 2018
- Ballantyne IV, Ford; Billings, Sharon A.
- The ISME Journal, Vol. 12, Issue 6
UCHIME improves sensitivity and speed of chimera detection
journal, June 2011
- Edgar, Robert C.; Haas, Brian J.; Clemente, Jose C.
- Bioinformatics, Vol. 27, Issue 16
The role of nitrogen in climate change and the impacts of nitrogen–climate interactions in the United States: foreword to thematic issue
journal, September 2012
- Suddick, Emma C.; Whitney, Penelope; Townsend, Alan R.
- Biogeochemistry, Vol. 114, Issue 1-3
Microbiome Profiling by Illumina Sequencing of Combinatorial Sequence-Tagged PCR Products
journal, October 2010
- Gloor, Gregory B.; Hummelen, Ruben; Macklaim, Jean M.
- PLoS ONE, Vol. 5, Issue 10
Nitrogen additions and microbial biomass: a meta-analysis of ecosystem studies
journal, October 2008
- Treseder, Kathleen K.
- Ecology Letters, Vol. 11, Issue 10
A theoretical model of C- and N-acquiring exoenzyme activities, which balances microbial demands during decomposition
journal, October 2012
- Moorhead, Daryl L.; Lashermes, Gwenaëlle; Sinsabaugh, Robert L.
- Soil Biology and Biochemistry, Vol. 53
Clarifying the interpretation of carbon use efficiency in soil through methods comparison
journal, January 2019
- Geyer, Kevin M.; Dijkstra, Paul; Sinsabaugh, Robert
- Soil Biology and Biochemistry, Vol. 128
Elemental stoichiometry of Fungi and Bacteria strains from grassland leaf litter
journal, September 2014
- Mouginot, Céline; Kawamura, Rika; Matulich, Kristin L.
- Soil Biology and Biochemistry, Vol. 76
Do we need to understand microbial communities to predict ecosystem function? A comparison of statistical models of nitrogen cycling processes
journal, January 2014
- Graham, Emily B.; Wieder, William R.; Leff, Jonathan W.
- Soil Biology and Biochemistry, Vol. 68
Contrasting soil fungal community responses to experimental nitrogen addition using the large subunit rRNA taxonomic marker and cellobiohydrolase I functional marker
journal, August 2014
- Mueller, Rebecca C.; Balasch, Monica M.; Kuske, Cheryl R.
- Molecular Ecology, Vol. 23, Issue 17
PCR Primers That Amplify Fungal rRNA Genes from Environmental Samples
journal, October 2000
- Borneman, J.; Hartin, R. J.
- Applied and Environmental Microbiology, Vol. 66, Issue 10
Soil Fungal Cellobiohydrolase I Gene ( cbhI ) Composition and Expression in a Loblolly Pine Plantation under Conditions of Elevated Atmospheric CO 2 and Nitrogen Fertilization
journal, March 2012
- Weber, Carolyn F.; Balasch, Monica Moya; Gossage, Zachary
- Applied and Environmental Microbiology, Vol. 78, Issue 11
Global negative effects of nitrogen deposition on soil microbes
journal, March 2018
- Zhang, Tian’an; Chen, Han Y. H.; Ruan, Honghua
- The ISME Journal, Vol. 12, Issue 7
Response of carbon utilization and enzymatic activities to nitrogen deposition in three forests of subtropical China
journal, April 2015
- Wang, Yong-Sheng; Cheng, Shu-Lan; Yu, Gui-Rui
- Canadian Journal of Forest Research, Vol. 45, Issue 4
Stoichiometry of microbial carbon use efficiency in soils
journal, May 2016
- Sinsabaugh, Robert L.; Turner, Benjamin L.; Talbot, Jennifer M.
- Ecological Monographs, Vol. 86, Issue 2
Pyrosequencing-Based Assessment of Soil pH as a Predictor of Soil Bacterial Community Structure at the Continental Scale
journal, June 2009
- Lauber, C. L.; Hamady, M.; Knight, R.
- Applied and Environmental Microbiology, Vol. 75, Issue 15
Soil microbial responses to nitrogen addition in arid ecosystems
journal, August 2015
- Sinsabaugh, Robert L.; Belnap, Jayne; Rudgers, Jennifer
- Frontiers in Microbiology, Vol. 6
Modeling Microbial Dynamics in Heterogeneous Environments: Growth on Soil Carbon Sources
journal, December 2011
- Resat, Haluk; Bailey, Vanessa; McCue, Lee Ann
- Microbial Ecology, Vol. 63, Issue 4
The Microbial Efficiency-Matrix Stabilization (MEMS) framework integrates plant litter decomposition with soil organic matter stabilization: do labile plant inputs form stable soil organic matter?
journal, February 2013
- Cotrufo, M. Francesca; Wallenstein, Matthew D.; Boot, Claudia M.
- Global Change Biology, Vol. 19, Issue 4
A global perspective on belowground carbon dynamics under nitrogen enrichment: Belowground C dynamics under N enrichment
journal, May 2010
- Liu, Lingli; Greaver, Tara L.
- Ecology Letters, Vol. 13, Issue 7
Changes in Fungal Community Composition in Response to Elevated Atmospheric CO2 and Nitrogen Fertilization Varies with Soil Horizon
journal, January 2013
- Weber, Carolyn F.; Vilgalys, Rytas; Kuske, Cheryl R.
- Frontiers in Microbiology, Vol. 4
Optimal metabolic regulation along resource stoichiometry gradients
journal, July 2017
- Manzoni, Stefano; Čapek, Petr; Mooshammer, Maria
- Ecology Letters, Vol. 20, Issue 9
Managing uncertainty in soil carbon feedbacks to climate change
journal, July 2016
- Bradford, Mark A.; Wieder, William R.; Bonan, Gordon B.
- Nature Climate Change, Vol. 6, Issue 8
Examining the global distribution of dominant archaeal populations in soil
journal, November 2010
- Bates, Scott T.; Berg-Lyons, Donna; Caporaso, J. Gregory
- The ISME Journal, Vol. 5, Issue 5
Soil bacterial and fungal community responses to nitrogen addition across soil depth and microhabitat in an arid shrubland
journal, September 2015
- Mueller, Rebecca C.; Belnap, Jayne; Kuske, Cheryl R.
- Frontiers in Microbiology, Vol. 6
Plant, microbial and ecosystem carbon use efficiencies interact to stabilize microbial growth as a fraction of gross primary production
journal, February 2017
- Sinsabaugh, Robert L.; Moorhead, Daryl L.; Xu, Xiaofeng
- New Phytologist, Vol. 214, Issue 4
Long-Term Experimental Nitrogen Deposition Alters the Composition of the Active Fungal Community in the Forest Floor
journal, January 2013
- Entwistle, Elizabeth M.; Zak, Donald R.; Edwards, Ivan P.
- Soil Science Society of America Journal, Vol. 77, Issue 5
UPARSE: highly accurate OTU sequences from microbial amplicon reads
journal, August 2013
- Edgar, Robert C.
- Nature Methods, Vol. 10, Issue 10
Rise and fall of nitrogen deposition in the United States
journal, June 2016
- Du, Enzai
- Proceedings of the National Academy of Sciences, Vol. 113, Issue 26
Rapid determination of 16S ribosomal RNA sequences for phylogenetic analyses.
journal, October 1985
- Lane, D. J.; Pace, B.; Olsen, G. J.
- Proceedings of the National Academy of Sciences, Vol. 82, Issue 20
Tree growth and soil acidification in response to 30 years of experimental nitrogen loading on boreal forest
journal, March 2006
- Hogberg, Peter; Fan, Houbao; Quist, Maud
- Global Change Biology, Vol. 12, Issue 3
The Global Stoichiometry of Litter Nitrogen Mineralization
journal, August 2008
- Manzoni, S.; Jackson, R. B.; Trofymow, J. A.
- Science, Vol. 321, Issue 5889
Complex response of the forest nitrogen cycle to climate change
journal, February 2012
- Bernal, S.; Hedin, L. O.; Likens, G. E.
- Proceedings of the National Academy of Sciences, Vol. 109, Issue 9
The human footprint in the carbon cycle of temperate and boreal forests
journal, June 2007
- Magnani, Federico; Mencuccini, Maurizio; Borghetti, Marco
- Nature, Vol. 447, Issue 7146
Uncertainties in CMIP5 Climate Projections due to Carbon Cycle Feedbacks
journal, January 2014
- Friedlingstein, Pierre; Meinshausen, Malte; Arora, Vivek K.
- Journal of Climate, Vol. 27, Issue 2
The application of ecological stoichiometry to plant–microbial–soil organic matter transformations
journal, May 2015
- Zechmeister-Boltenstern, Sophie; Keiblinger, Katharina Maria; Mooshammer, Maria
- Ecological Monographs, Vol. 85, Issue 2
Linking microbial community structure and microbial processes: an empirical and conceptual overview
journal, September 2015
- Bier, Raven L.; Bernhardt, Emily S.; Boot, Claudia M.
- FEMS Microbiology Ecology, Vol. 91, Issue 10
Global assessment of nitrogen deposition effects on terrestrial plant diversity: a synthesis
journal, January 2010
- Bobbink, R.; Hicks, K.; Galloway, J.
- Ecological Applications, Vol. 20, Issue 1
Altered patterns of soil carbon substrate usage and heterotrophic respiration in a pine forest with elevated CO 2 and N fertilization
journal, May 2008
- Billings, Sharon A.; Ziegler, Susan E.
- Global Change Biology, Vol. 14, Issue 5
Short-term effects of fertilization on photosynthesis and leaf morphology of field-grown loblolly pine following long-term exposure to elevated CO2 concentration
journal, April 2008
- Maier, C. A.; Palmroth, S.; Ward, E.
- Tree Physiology, Vol. 28, Issue 4
QIIME allows analysis of high-throughput community sequencing data
journal, April 2010
- Caporaso, J. Gregory; Kuczynski, Justin; Stombaugh, Jesse
- Nature Methods, Vol. 7, Issue 5
Vector analysis of ecoenzyme activities reveal constraints on coupled C, N and P dynamics
journal, February 2016
- Moorhead, Daryl L.; Sinsabaugh, Robert L.; Hill, Brian H.
- Soil Biology and Biochemistry, Vol. 93
Growth of saprotrophic fungi and bacteria in soil: Growth of saprotrophic fungi and bacteria in soil
journal, April 2011
- Rousk, Johannes; Bååth, Erland
- FEMS Microbiology Ecology, Vol. 78, Issue 1
Nitrogen limitation of decomposition and decay: How can it occur?
journal, December 2017
- Averill, Colin; Waring, Bonnie
- Global Change Biology, Vol. 24, Issue 4
Re-assessment of plant carbon dynamics at the Duke free-air CO2 enrichment site: interactions of atmospheric [CO2] with nitrogen and water availability over stand development
journal, November 2009
- McCarthy, Heather R.; Oren, Ram; Johnsen, Kurt H.
- New Phytologist, Vol. 185, Issue 2
Microbial functional diversity enhances predictive models linking environmental parameters to ecosystem properties
journal, July 2015
- Powell, Jeff R.; Welsh, Allana; Hallin, Sara
- Ecology, Vol. 96, Issue 7
Phenol oxidase, peroxidase and organic matter dynamics of soil
journal, March 2010
- Sinsabaugh, Robert L.
- Soil Biology and Biochemistry, Vol. 42, Issue 3
Continuing Acidification of Organic Soils across the Northeastern USA: 1984–2001
journal, January 2009
- Warby, Richard A. F.; Johnson, Chris E.; Driscoll, Charles T.
- Soil Science Society of America Journal, Vol. 73, Issue 1
Soil Microbial Community Responses to Multiple Experimental Climate Change Drivers
journal, December 2009
- Castro, H. F.; Classen, A. T.; Austin, E. E.
- Applied and Environmental Microbiology, Vol. 76, Issue 4
Reduction of forest soil respiration in response to nitrogen deposition
journal, April 2010
- Janssens, I. A.; Dieleman, W.; Luyssaert, S.
- Nature Geoscience, Vol. 3, Issue 5
A global analysis of soil acidification caused by nitrogen addition
journal, February 2015
- Tian, Dashuan; Niu, Shuli
- Environmental Research Letters, Vol. 10, Issue 2
Key ecological responses to nitrogen are altered by climate change
journal, August 2016
- Greaver, T. L.; Clark, C. M.; Compton, J. E.
- Nature Climate Change, Vol. 6, Issue 9
Nitrogen Additions and Litter Decomposition: a Meta-Analysis
journal, December 2005
- Knorr, M.; Frey, S. D.; Curtis, P. S.
- Ecology, Vol. 86, Issue 12
The changing global carbon cycle: linking plant-soil carbon dynamics to global consequences
journal, September 2009
- Stuart Chapin III, F.; McFarland, Jack; David McGuire, A.
- Journal of Ecology, Vol. 97, Issue 5
The diversity and biogeography of soil bacterial communities
journal, January 2006
- Fierer, N.; Jackson, R. B.
- Proceedings of the National Academy of Sciences, Vol. 103, Issue 3
Different responses of soil respiration and its components to nitrogen addition among biomes: a meta-analysis
journal, April 2014
- Zhou, Lingyan; Zhou, Xuhui; Zhang, Baocheng
- Global Change Biology, Vol. 20, Issue 7
Climate change decreases nitrogen pools and mineralization rates in northern hardwood forests
journal, March 2016
- Durán, Jorge; Morse, Jennifer L.; Groffman, Peter M.
- Ecosphere, Vol. 7, Issue 3
A New Conceptual Model of Nitrogen Saturation Based on Experimental Nitrogen Addition to an Oak Forest
journal, March 2011
- Lovett, Gary M.; Goodale, Christine L.
- Ecosystems, Vol. 14, Issue 4
Endemism and functional convergence across the North American soil mycobiome
journal, April 2014
- Talbot, J. M.; Bruns, T. D.; Taylor, J. W.
- Proceedings of the National Academy of Sciences, Vol. 111, Issue 17
Ecoenzymatic Stoichiometry and Ecological Theory
journal, December 2012
- Sinsabaugh, Robert L.; Follstad Shah, Jennifer J.
- Annual Review of Ecology, Evolution, and Systematics, Vol. 43, Issue 1
Carbon use efficiency of microbial communities: stoichiometry, methodology and modelling
journal, April 2013
- Sinsabaugh, Robert L.; Manzoni, Stefano; Moorhead, Daryl L.
- Ecology Letters, Vol. 16, Issue 7
Adjustment of microbial nitrogen use efficiency to carbon:nitrogen imbalances regulates soil nitrogen cycling
journal, April 2014
- Mooshammer, Maria; Wanek, Wolfgang; Hämmerle, Ieda
- Nature Communications, Vol. 5, Issue 1
Figures / Tables found in this record:
Figures/Tables have been extracted from DOE-funded journal article accepted manuscripts.