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Title: Biotin Attachment Domain-Containing proteins, inhibitors of ACCase, are regulated by Wrinkled1

Abstract

WRINKLED1 (WRI1) is a transcriptional activator that binds to AW boxes in the promoters of many genes from central metabolism and FA synthesis, resulting in their transcription. BIOTIN ATTACHMENT DOMAIN-CONTAINING (BADC) proteins are homologs of BIOTIN CARBOXYL CARRIER PROTEIN (BCCP) that lack a biotin-attachment domain and are therefore inactive. In the presence of excess FA, BADC1 and BADC3 are primarily responsible for the observed long-term irreversible inhibition of ACETYL-COA CARBOXYLASE (ACCase), and consequently FA synthesis. Purified WRI1 bound with high affinity ( Kds in the low nanomolar range) to canonical AW-boxes from the promoters of all three BADC genes. Consistent with this observation, the expression of BADC1, BADC2 and BADC3 genes and BADC1 protein levels were reduced in wri1-1 relative to wild type (WT), and BADCs gene expression and BADC1 protein levels also were elevated upon WRI1 overexpression. The double mutant badc1badc2 phenocopied wri1-1 with respect to both reduction in root length, and elevation of indole-3-acetic acid-Asp (IAA-Asp) levels relative to WT. Overexpression of BADC1 in wri1-1 decreased its IAA-Asp and partially rescued its short-root phenotype demonstrating a role for BADCs in seedling establishment. In conclusion, that WRI1 positively regulates genes encoding both FA synthesis and BADCs i.e., conditional inhibitorsmore » of FA synthesis, represents a coordinated mechanism to achieve lipid homeostasis in which plants couple the transcription their FA synthetic capacity with their capacity to biochemically downregulate it.« less

Authors:
ORCiD logo [1];  [1];  [1];  [1];  [1];  [1]
  1. Brookhaven National Lab. (BNL), Upton, NY (United States)
Publication Date:
Research Org.:
Brookhaven National Lab. (BNL), Upton, NY (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Basic Energy Sciences (BES) (SC-22)
OSTI Identifier:
1525402
Report Number(s):
BNL-211766-2019-JAAM
Grant/Contract Number:  
SC0012704
Resource Type:
Accepted Manuscript
Journal Name:
Plant Physiology
Additional Journal Information:
Journal Name: Plant Physiology
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; Metabolic regulation; homeostatic mechanism; lipid metabolism; triacylglycerol

Citation Formats

Liu, Hui, Zhai, Zhiyang, Kuczynski, Kate, Keereetaweep, Jantana, Schwender, Jorg, and Shanklin, John. Biotin Attachment Domain-Containing proteins, inhibitors of ACCase, are regulated by Wrinkled1. United States: N. p., 2019. Web. doi:10.1101/634550.
Liu, Hui, Zhai, Zhiyang, Kuczynski, Kate, Keereetaweep, Jantana, Schwender, Jorg, & Shanklin, John. Biotin Attachment Domain-Containing proteins, inhibitors of ACCase, are regulated by Wrinkled1. United States. doi:10.1101/634550.
Liu, Hui, Zhai, Zhiyang, Kuczynski, Kate, Keereetaweep, Jantana, Schwender, Jorg, and Shanklin, John. Wed . "Biotin Attachment Domain-Containing proteins, inhibitors of ACCase, are regulated by Wrinkled1". United States. doi:10.1101/634550.
@article{osti_1525402,
title = {Biotin Attachment Domain-Containing proteins, inhibitors of ACCase, are regulated by Wrinkled1},
author = {Liu, Hui and Zhai, Zhiyang and Kuczynski, Kate and Keereetaweep, Jantana and Schwender, Jorg and Shanklin, John},
abstractNote = {WRINKLED1 (WRI1) is a transcriptional activator that binds to AW boxes in the promoters of many genes from central metabolism and FA synthesis, resulting in their transcription. BIOTIN ATTACHMENT DOMAIN-CONTAINING (BADC) proteins are homologs of BIOTIN CARBOXYL CARRIER PROTEIN (BCCP) that lack a biotin-attachment domain and are therefore inactive. In the presence of excess FA, BADC1 and BADC3 are primarily responsible for the observed long-term irreversible inhibition of ACETYL-COA CARBOXYLASE (ACCase), and consequently FA synthesis. Purified WRI1 bound with high affinity (Kds in the low nanomolar range) to canonical AW-boxes from the promoters of all three BADC genes. Consistent with this observation, the expression of BADC1, BADC2 and BADC3 genes and BADC1 protein levels were reduced in wri1-1 relative to wild type (WT), and BADCs gene expression and BADC1 protein levels also were elevated upon WRI1 overexpression. The double mutant badc1badc2 phenocopied wri1-1 with respect to both reduction in root length, and elevation of indole-3-acetic acid-Asp (IAA-Asp) levels relative to WT. Overexpression of BADC1 in wri1-1 decreased its IAA-Asp and partially rescued its short-root phenotype demonstrating a role for BADCs in seedling establishment. In conclusion, that WRI1 positively regulates genes encoding both FA synthesis and BADCs i.e., conditional inhibitors of FA synthesis, represents a coordinated mechanism to achieve lipid homeostasis in which plants couple the transcription their FA synthetic capacity with their capacity to biochemically downregulate it.},
doi = {10.1101/634550},
journal = {Plant Physiology},
number = ,
volume = ,
place = {United States},
year = {2019},
month = {6}
}

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