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Title: Active elastohydrodynamics of vesicles in narrow blind constrictions

Abstract

Fluid-resistance limited transport of vesicles through narrow constrictions is a recurring theme in many biological and engineering applications. Inspired by the motor-driven movement of soft membrane-bound vesicles into closed neuronal dendritic spines, here we study this problem using a combination of passive three-dimensional simulations and a simplified semianalytical theory for the active transport of vesicles forced through constrictions by molecular motors. We show that the motion of these objects is characterized by two dimensionless quantities related to the geometry and to the strength of forcing relative to the vesicle elasticity. We use numerical simulations to characterize the transit time for a vesicle forced by fluid pressure through a constriction in a channel and find that relative to an open channel, transport into a blind end leads to the formation of a smaller forward-flowing lubrication layer that strongly impedes motion. When the fluid pressure forcing is complemented by forces due to molecular motors that are responsible for vesicle trafficking into dendritic spines, we find that the competition between motor forcing and fluid drag results in multistable dynamics reminiscent of the real system. Our study highlights the role of nonlocal hydrodynamic effects in determining the kinetics of vesicular transport in constricted geometries.

Authors:
 [1];  [2];  [3];  [1];  [1]
+ Show Author Affiliations
  1. Harvard Univ., Cambridge, MA (United States)
  2. Eindhoven Univ. of Technology (Netherlands); National Centre for Scientific Research-Mixed Organizations (CNRS-UMR), Paris (France)
  3. Eindhoven Univ. of Technology (Netherlands); Helmholtz Inst. Erlangen-Nurnberg for Renewable Energy, Nurnberg (Germany)
Publication Date:
Research Org.:
Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
Sponsoring Org.:
USDOE Office of Science (SC)
OSTI Identifier:
1525248
Grant/Contract Number:  
AC02-05CH11231
Resource Type:
Accepted Manuscript
Journal Name:
Physical Review Fluids
Additional Journal Information:
Journal Volume: 2; Journal Issue: 11; Journal ID: ISSN 2469-990X
Publisher:
American Physical Society (APS)
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES

Citation Formats

Fai, T. G., Kusters, R., Harting, J., Rycroft, C. H., and Mahadevan, L. Active elastohydrodynamics of vesicles in narrow blind constrictions. United States: N. p., 2017. Web. doi:10.1103/PhysRevFluids.2.113601.
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Fai, T. G., Kusters, R., Harting, J., Rycroft, C. H., & Mahadevan, L. Active elastohydrodynamics of vesicles in narrow blind constrictions. United States. https://doi.org/10.1103/PhysRevFluids.2.113601
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Fai, T. G., Kusters, R., Harting, J., Rycroft, C. H., and Mahadevan, L. Fri . "Active elastohydrodynamics of vesicles in narrow blind constrictions". United States. https://doi.org/10.1103/PhysRevFluids.2.113601. https://www.osti.gov/servlets/purl/1525248.
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@article{osti_1525248,
title = {Active elastohydrodynamics of vesicles in narrow blind constrictions},
author = {Fai, T. G. and Kusters, R. and Harting, J. and Rycroft, C. H. and Mahadevan, L.},
abstractNote = {Fluid-resistance limited transport of vesicles through narrow constrictions is a recurring theme in many biological and engineering applications. Inspired by the motor-driven movement of soft membrane-bound vesicles into closed neuronal dendritic spines, here we study this problem using a combination of passive three-dimensional simulations and a simplified semianalytical theory for the active transport of vesicles forced through constrictions by molecular motors. We show that the motion of these objects is characterized by two dimensionless quantities related to the geometry and to the strength of forcing relative to the vesicle elasticity. We use numerical simulations to characterize the transit time for a vesicle forced by fluid pressure through a constriction in a channel and find that relative to an open channel, transport into a blind end leads to the formation of a smaller forward-flowing lubrication layer that strongly impedes motion. When the fluid pressure forcing is complemented by forces due to molecular motors that are responsible for vesicle trafficking into dendritic spines, we find that the competition between motor forcing and fluid drag results in multistable dynamics reminiscent of the real system. Our study highlights the role of nonlocal hydrodynamic effects in determining the kinetics of vesicular transport in constricted geometries.},
doi = {10.1103/PhysRevFluids.2.113601},
journal = {Physical Review Fluids},
number = 11,
volume = 2,
place = {United States},
year = {Fri Nov 10 00:00:00 EST 2017},
month = {Fri Nov 10 00:00:00 EST 2017}
}
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Journal Article:
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Publisher's Version of Record
https://doi.org/10.1103/PhysRevFluids.2.113601
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Cited by: 10 works
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Figures / Tables:

Figure 1 Figure 1: (Color online) (a) Timelapse images and (b) corresponding kymograph from a dual-color timelapse recording showing the actomyosin-based transportation of an endosome through the neck of a neuronal dendritic spine over 40 – 60 seconds (adapted from [11] and available under CC BY NC ND license). The bright colormore » represents the endosome and the curve in (b) indicates the contour of the spine. The region of high intensity at the base of the spine represents a pool of endosomes, from which the kymograph shows one entrance event. (c) Our corresponding model system for the forced translocation of a vesicle through a narrow constriction. (d) Schematic of the motor model used which contain two species of motors, one pushing up the channel, and another pushing down the channel.« less
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    Simulating Transport of Soft Matter in Micro/Nano Channel Flows with Dissipative Particle Dynamics
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    • Advanced Theory and Simulations, Vol. 2, Issue 2
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