In situ liquid cell crystallization and imaging of thiamethoxam by helium ion microscopy

Belianinov, Alex; Pawlicki, Alison A.; Burch, Matt J.; Kim, Songkil; Ievlev, Anton; Fowler, Jeff; Ovchinnikova, Olga S.

doi:10.1116/1.5040849

Title: In situ liquid cell crystallization and imaging of thiamethoxam by helium ion microscopy

Abstract

Pest insect control is an essential component for crop protection and public health. Neonicotinoids are a relatively new class of insecticides developed in the last four decades. Thiamethoxam, a member of the neonicotinoid class, has shown outstanding potency for crop protection against a variety of piercing-sucking pests. However, its use in industrial-volume packing and transportation is complicated by crystallization dynamics. In this work, a helium ion microscope (HIM) with a Protochips liquid cell was utilized to crystallize and image thiamethoxam in situ. The results of the study illustrate the growth and morphology of the thiamethoxam crystals at different He⁺ exposure doses, which is markedly different from what has been typically observed. Energy-dispersive x-ray spectroscopy results confirm the presence of the thiamethoxam on the liquid cell membrane. This imaging study illustrates the HIM ability to image and induce the crystallization in soft materials in liquid environments, and attempts to shed light onto the key processes involved in liquid imaging.

Authors:

^[1]; Pawlicki, Alison A. ^[2];

^[1];

^[3];

^[1]; Fowler, Jeff ^[4];

^[1]

Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Univ. of Tennessee, Knoxville, TN (United States)
Pusan National Univ., Busan (South Korea)
Syngenta Crop Protection, Greensboro, NC (United States)

Publication Date:: 2018-08-31

Research Org.:: Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)

Sponsoring Org.:: USDOE

OSTI Identifier:: 1524876

Grant/Contract Number:: AC05-00OR22725

Resource Type:: Accepted Manuscript

Journal Name:: Journal of Vacuum Science and Technology. B, Nanotechnology and Microelectronics

Additional Journal Information:: Journal Volume: 36; Journal Issue: 5; Journal ID: ISSN 2166-2746

Publisher:: American Vacuum Society/AIP

Country of Publication:: United States

Language:: English

Subject:: 60 APPLIED LIFE SCIENCES; 77 NANOSCIENCE AND NANOTECHNOLOGY

Citation Formats


                    Belianinov, Alex, Pawlicki, Alison A., Burch, Matt J., Kim, Songkil, Ievlev, Anton, Fowler, Jeff, and Ovchinnikova, Olga S.. In situ liquid cell crystallization and imaging of thiamethoxam by helium ion microscopy.  United States: N. p., 2018. 
Web.  doi:10.1116/1.5040849.

Copy to clipboard


                    Belianinov, Alex, Pawlicki, Alison A., Burch, Matt J., Kim, Songkil, Ievlev, Anton, Fowler, Jeff, & Ovchinnikova, Olga S.. In situ liquid cell crystallization and imaging of thiamethoxam by helium ion microscopy.  United States.  https://doi.org/10.1116/1.5040849

Copy to clipboard


                    Belianinov, Alex, Pawlicki, Alison A., Burch, Matt J., Kim, Songkil, Ievlev, Anton, Fowler, Jeff, and Ovchinnikova, Olga S.. Fri .  
"In situ liquid cell crystallization and imaging of thiamethoxam by helium ion microscopy".  United States.  https://doi.org/10.1116/1.5040849.  https://www.osti.gov/servlets/purl/1524876.

Copy to clipboard


                    
@article{osti_1524876,

  title        = {In situ liquid cell crystallization and imaging of thiamethoxam by helium ion microscopy},

  author       = {Belianinov, Alex and Pawlicki, Alison A. and Burch, Matt J. and Kim, Songkil and Ievlev, Anton and Fowler, Jeff and Ovchinnikova, Olga S.},

  abstractNote = {Pest insect control is an essential component for crop protection and public health. Neonicotinoids are a relatively new class of insecticides developed in the last four decades. Thiamethoxam, a member of the neonicotinoid class, has shown outstanding potency for crop protection against a variety of piercing-sucking pests. However, its use in industrial-volume packing and transportation is complicated by crystallization dynamics. In this work, a helium ion microscope (HIM) with a Protochips liquid cell was utilized to crystallize and image thiamethoxam in situ. The results of the study illustrate the growth and morphology of the thiamethoxam crystals at different He+ exposure doses, which is markedly different from what has been typically observed. Energy-dispersive x-ray spectroscopy results confirm the presence of the thiamethoxam on the liquid cell membrane. This imaging study illustrates the HIM ability to image and induce the crystallization in soft materials in liquid environments, and attempts to shed light onto the key processes involved in liquid imaging.},

  doi          = {10.1116/1.5040849},

  journal      = {Journal of Vacuum Science and Technology. B, Nanotechnology and Microelectronics},

  number       = 5,

  volume       = 36,

  place        = {United States},

  year         = {2018},

  month        = {8}

}

Copy to clipboard

Journal Article:

Free Publicly Available Full Text

Accepted Manuscript (DOE)

Publisher's Version of Record

https://doi.org/10.1116/1.5040849

Other availability

Search WorldCat to find libraries that may hold this journal

Citation Metrics:

Cited by: 1 work

Citation information provided by
Web of Science

Figures / Tables:

FIG. 1: HIM, with the use of e⁻flood gun, displaying TMX crystals on a glass surface. (a) HIM image of many large crystallites that form upon drop casting an aqueous supersaturated solution of TMX and drying. (b) HIM image of two particular TMX crystals. (c) HIM image with amore »

All figures and tables (5 total)

Save / Share:

Export Metadata

Save to My Library

Figures / Tables found in this record:

Figures/Tables have been extracted from DOE-funded journal article accepted manuscripts.

Similar Records in DOE PAGES and OSTI.GOV collections:

Developmental neurotoxicity of different pesticides in PC-12 cells in vitro

Journal Article Christen, Verena ; Rusconi, Manuel ; Crettaz, Pierre ; ... - Toxicology and Applied Pharmacology

The detection of developmental neurotoxicity (DNT) of chemicals has high relevance for protection of human health. However, DNT of many pesticides is only little known. Furthermore, validated in vitro systems for assessment of DNT are not well established. Here we employed the rat phaeochromocytoma cell line PC-12 to evaluate DNT of 18 frequently used pesticides of different classes, including neonicotinoids, pyrethroids, organophosphates, organochlorines, as well as quaternary ammonium compounds, the organic compound used in pesticides, piperonyl butoxide, as well as the insect repellent diethyltoluamide (DEET). We determined the outgrowth of neurites in PC-12 cells co-treated with nerve growth factor andmore »« less
https://doi.org/10.1016/J.TAAP.2017.03.027
The binding properties of cycloxaprid on insect native nAChRs partially explain the low cross‐resistance with imidacloprid in Nilaparvata lugens

Journal Article Zhang, Yixi ; Xu, Xiaoyong ; Bao, Haibo ; ... - Pest Management Science

Abstract BACKGROUND Neonicotinoids, such as imidacloprid, are selective agonists of insect nicotinic acetylcholine receptors (nAChRs) used to control Nilaparvata lugens , a major rice insect pest. High imidacloprid resistance has been reported in N. lugens both in the laboratory and in the field. Cycloxaprid (CYC), an oxa‐bridged cis ‐nitromethylene neonicotinoid, showed high insecticidal activity against N. lugens and low cross‐resistance in imidacloprid‐resistant strains and field populations. RESULTS Binding studies demonstrated that imidacloprid has two binding sites with different affinities ( K _d = 3.18 ± 0.43 p m and 1.78 ± 0.19 n m ) in N. lugens nAChRs. CYCmore »« less
Cited by 13
https://doi.org/10.1002/ps.5108
Bacillus thuringiensisCry1Da_7 and Cry1B.868 Protein Interactions with Novel Receptors Allow Control of Resistant Fall Armyworms,Spodoptera frugiperda(J.E. Smith)

Journal Article Wang, Yanfei ; Wang, Jinling ; Fu, Xiaoran ; ... - Applied and Environmental Microbiology

Two new modified Bacillus thuringiensis (Bt) proteins, Cry1Da_7 and Cry1B.868, with activity against fall armyworms (FAW), Spodoptera frugiperda (J.E. Smith), were evaluated for their potential to bind new insect receptors compared to proteins currently deployed as plant-incorporated protectants (PIPs) in row crops. Results from resistant insect bioassays, disabled insecticidal protein (DIP) bioassays, and cell-based assays using insect cells expressing individual receptors demonstrate that receptor utilizations of the newly modified Cry1Da_7 and Cry1B.868 proteins are distinct from each other and from those of commercially available Bt proteins such as Cry1F, Cry1A.105, Cry2Ab, and Vip3A. Accordingly, these two proteins target different insectmore »« less
Cited by 23
https://doi.org/10.1128/aem.00579-19

Full Text Available
Atomic interactions of neonicotinoid agonists with AChBP: Molecular recognition of the distinctive electronegative pharmacophore

Journal Article Talley, Todd T ; Harel, Michal ; Hibbs, Ryan E ; ... - Proc. Natl. Acad. Sci. USA

Acetylcholine-binding proteins (AChBPs) from mollusks are suitable structural and functional surrogates of the nicotinic acetylcholine receptors when combined with transmembrane spans of the nicotinic receptor. These proteins assemble as a pentamer with identical ACh binding sites at the subunit interfaces and show ligand specificities resembling those of the nicotinic receptor for agonists and antagonists. A subset of ligands, termed the neonicotinoids, exhibit specificity for insect nicotinic receptors and selective toxicity as insecticides. AChBPs are of neither mammalian nor insect origin and exhibit a distinctive pattern of selectivity for the neonicotinoid ligands. We define here the binding orientation and determinants ofmore »« less
https://doi.org/10.1073/pnas.0802197105
Allergy arising from exposure to airborne contaminants in an insect rearing facility: Health effects and exposure control

Technical Report Wolff, D

In agricultural crop improvement, yield under various stress conditions and limiting factors is assessed experimentally. Of the stresses on plants which affect yield are those due to insects. Ostrinia nubilalis, the European corn borer (corn borer) is a major pest in sweet and field corn in the U.S. There are many ways to fight crop pests such as the corn borer, including (1) application of chemical insecticides, (2) application of natural predators and, (3) improving crop resistance through plant genetics programs. Randomized field trials are used to determine the effectiveness of pest management programs. These trials frequently consist of randomlymore »« less
https://doi.org/10.2172/576050

Full Text Available

Similar Records