Modular Engineering of Biomass Degradation Pathways
Abstract
Production of fuels and chemicals from renewable lignocellulosic feedstocks is a promising alternative to petroleum-derived compounds. Because of the complexity of lignocellulosic feedstocks, microbial conversion of all potential substrates will require substantial metabolic engineering. Non-model microbes offer desirable physiological traits, but also increase the difficulty of heterologous pathway engineering and optimization. The development of modular design principles that allow metabolic pathways to be used in a variety of novel microbes with minimal strain-specific optimization will enable the rapid construction of microbes for commercial production of biofuels and bioproducts. In this review, we discuss variability of lignocellulosic feedstocks, pathways for catabolism of lignocellulose-derived compounds, challenges to heterologous engineering of catabolic pathways, and opportunities to apply modular pathway design. Implementation of these approaches will simplify the process of modifying non-model microbes to convert diverse lignocellulosic feedstocks.
- Authors:
-
- Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
- Publication Date:
- Research Org.:
- Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC), Biological and Environmental Research (BER)
- OSTI Identifier:
- 1523739
- Grant/Contract Number:
- AC05-00OR22725
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Processes
- Additional Journal Information:
- Journal Volume: 7; Journal Issue: 4; Journal ID: ISSN 2227-9717
- Publisher:
- Multidisciplinary Digital Publishing Institute (MDPI)
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 09 BIOMASS FUELS; metabolic engineering; lignin valorization; lignocellulose; biofuels; hemicellulose
Citation Formats
Chaves, Julie E., Presley, Gerald N., and Michener, Joshua K. Modular Engineering of Biomass Degradation Pathways. United States: N. p., 2019.
Web. doi:10.3390/pr7040230.
Chaves, Julie E., Presley, Gerald N., & Michener, Joshua K. Modular Engineering of Biomass Degradation Pathways. United States. https://doi.org/10.3390/pr7040230
Chaves, Julie E., Presley, Gerald N., and Michener, Joshua K. Mon .
"Modular Engineering of Biomass Degradation Pathways". United States. https://doi.org/10.3390/pr7040230. https://www.osti.gov/servlets/purl/1523739.
@article{osti_1523739,
title = {Modular Engineering of Biomass Degradation Pathways},
author = {Chaves, Julie E. and Presley, Gerald N. and Michener, Joshua K.},
abstractNote = {Production of fuels and chemicals from renewable lignocellulosic feedstocks is a promising alternative to petroleum-derived compounds. Because of the complexity of lignocellulosic feedstocks, microbial conversion of all potential substrates will require substantial metabolic engineering. Non-model microbes offer desirable physiological traits, but also increase the difficulty of heterologous pathway engineering and optimization. The development of modular design principles that allow metabolic pathways to be used in a variety of novel microbes with minimal strain-specific optimization will enable the rapid construction of microbes for commercial production of biofuels and bioproducts. In this review, we discuss variability of lignocellulosic feedstocks, pathways for catabolism of lignocellulose-derived compounds, challenges to heterologous engineering of catabolic pathways, and opportunities to apply modular pathway design. Implementation of these approaches will simplify the process of modifying non-model microbes to convert diverse lignocellulosic feedstocks.},
doi = {10.3390/pr7040230},
journal = {Processes},
number = 4,
volume = 7,
place = {United States},
year = {Mon Apr 01 00:00:00 EDT 2019},
month = {Mon Apr 01 00:00:00 EDT 2019}
}
Web of Science
Figures / Tables:
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Works referencing / citing this record:
Engineered Pseudomonas putida KT2440 co-utilizes galactose and glucose
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- Biotechnology for Biofuels, Vol. 12, Issue 1
Engineered Pseudomonas putida KT2440 co-utilizes galactose and glucose
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