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Title: In Situ Engineering of Intracellular Hemoglobin for Implantable High-Performance Biofuel Cells

Abstract

The key challenge for the broad application of implantable biofuel cells (BFCs) is to achieve inorganic–organic composite biocompatibility while improving the activity and selectivity of the catalysts. We have fabricated nanoengineered red blood cells (NERBCs) by an environmentally friendly method by using red blood cells as the raw material and hemoglobin (Hb) embedded with ultrasmall hydroxyapatite (HAP, Ca 10(PO 4) 6(OH) 2) as the functional BFC cathode material. The NERBCs showed greatly enhanced cell performance with high electrocatalytic activity, stability, and selectivity. The NERBCs maintained the original biological properties of the natural cell, while enhancing the catalytic oxygen reduction reaction (ORR) through the interaction between -OH groups in HAP and the Hb in RBCs. Finally, they also enabled direct electron transportation, eliminating the need for an electron-transfer mediator, and showed catalytic inactivity for glucose oxidation, thus potentially enabling the development of separator-free BFCs.

Authors:
 [1];  [1];  [1];  [2];  [3];  [1];  [1];  [4];  [1];  [3];  [1];  [2]
  1. Henan Normal Univ., Xinxiang, Henan (China). Collaborative Innovation Center of Henan Province for Green Manufacturing of Fine Chemicals, Key Lab. of Green Chemical Media and Reactions, Ministry of Education,School of Chemistry and Chemical Engineering and College of Physics and Materials Science
  2. Argonne National Lab. (ANL), Argonne, IL (United States). Chemical Sciences and Engineering Division
  3. Univ. of Waterloo, ON (Canada). Waterloo Inst. for Nanotechnology, Waterloo Inst. for Sustainable Energy
  4. Argonne National Lab. (ANL), Argonne, IL (United States). Chemical Sciences and Engineering Division; Univ. of Waterloo, ON (Canada). Waterloo Inst. for Nanotechnology, Waterloo Inst. for Sustainable Energy
Publication Date:
Research Org.:
Argonne National Lab. (ANL), Argonne, IL (United States)
Sponsoring Org.:
National Natural Science Foundation of China (NNSFC); USDOE Office of Energy Efficiency and Renewable Energy (EERE), Vehicle Technologies Office (EE-3V)
OSTI Identifier:
1523652
Grant/Contract Number:  
AC02-06CH11357; 16IRTSTHN004
Resource Type:
Accepted Manuscript
Journal Name:
Angewandte Chemie
Additional Journal Information:
Journal Volume: 131; Journal Issue: 20; Journal ID: ISSN 0044-8249
Publisher:
German Chemical Society
Country of Publication:
United States
Language:
English
Subject:
09 BIOMASS FUELS; high performance electrocatalyst; implantable biofuel cells; in situ engineering of hemoglobin; oxygen reduction reaction; red blood cells

Citation Formats

Chen, Huifeng, Bai, Zhengyu, Dai, Xianqi, Zeng, Xiaoqiao, Cano, Zachary P., Xie, Xiaoxiao, Zhao, Mingyu, Li, Matthew, Wang, He, Chen, Zhongwei, Yang, Lin, and Lu, Jun. In Situ Engineering of Intracellular Hemoglobin for Implantable High-Performance Biofuel Cells. United States: N. p., 2019. Web. doi:10.1002/ange.201902073.
Chen, Huifeng, Bai, Zhengyu, Dai, Xianqi, Zeng, Xiaoqiao, Cano, Zachary P., Xie, Xiaoxiao, Zhao, Mingyu, Li, Matthew, Wang, He, Chen, Zhongwei, Yang, Lin, & Lu, Jun. In Situ Engineering of Intracellular Hemoglobin for Implantable High-Performance Biofuel Cells. United States. doi:10.1002/ange.201902073.
Chen, Huifeng, Bai, Zhengyu, Dai, Xianqi, Zeng, Xiaoqiao, Cano, Zachary P., Xie, Xiaoxiao, Zhao, Mingyu, Li, Matthew, Wang, He, Chen, Zhongwei, Yang, Lin, and Lu, Jun. Sat . "In Situ Engineering of Intracellular Hemoglobin for Implantable High-Performance Biofuel Cells". United States. doi:10.1002/ange.201902073.
@article{osti_1523652,
title = {In Situ Engineering of Intracellular Hemoglobin for Implantable High-Performance Biofuel Cells},
author = {Chen, Huifeng and Bai, Zhengyu and Dai, Xianqi and Zeng, Xiaoqiao and Cano, Zachary P. and Xie, Xiaoxiao and Zhao, Mingyu and Li, Matthew and Wang, He and Chen, Zhongwei and Yang, Lin and Lu, Jun},
abstractNote = {The key challenge for the broad application of implantable biofuel cells (BFCs) is to achieve inorganic–organic composite biocompatibility while improving the activity and selectivity of the catalysts. We have fabricated nanoengineered red blood cells (NERBCs) by an environmentally friendly method by using red blood cells as the raw material and hemoglobin (Hb) embedded with ultrasmall hydroxyapatite (HAP, Ca10(PO4)6(OH)2) as the functional BFC cathode material. The NERBCs showed greatly enhanced cell performance with high electrocatalytic activity, stability, and selectivity. The NERBCs maintained the original biological properties of the natural cell, while enhancing the catalytic oxygen reduction reaction (ORR) through the interaction between -OH groups in HAP and the Hb in RBCs. Finally, they also enabled direct electron transportation, eliminating the need for an electron-transfer mediator, and showed catalytic inactivity for glucose oxidation, thus potentially enabling the development of separator-free BFCs.},
doi = {10.1002/ange.201902073},
journal = {Angewandte Chemie},
number = 20,
volume = 131,
place = {United States},
year = {2019},
month = {5}
}

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