skip to main content
DOE PAGES title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Lipopolysaccharide O-antigen delays plant innate immune recognition of Xylella fastidiosa

Abstract

Lipopolysaccharides (LPS) are among the known pathogen-associated molecular patterns (PAMPs). LPSs are potent elicitors of PAMP-triggered immunity (PTI), and bacteria have evolved intricate mechanisms to dampen PTI. Here we demonstrate that Xylella fastidiosa (Xf), a hemibiotrophic plant pathogenic bacterium, possesses a long chain O-antigen that enables it to delay initial plant recognition, thereby allowing it to effectively skirt initial elicitation of innate immunity and establish itself in the host. Lack of the O-antigen modifies plant perception of Xf and enables elicitation of hallmarks of PTI, such as ROS production specifically in the plant xylem tissue compartment, a tissue not traditionally considered a spatial location of PTI. To explore translational applications of our findings, we demonstrate that pre-treatment of plants with Xf LPS primes grapevine defenses to confer tolerance to Xf challenge.

Authors:
 [1];  [2]; ORCiD logo [3];  [4]; ORCiD logo [4];  [3];  [3];  [1]; ORCiD logo [4];  [1]
  1. Univ. of California, Riverside, CA (United States). Dept. of Microbiology and Plant Pathology
  2. Univ. of California, Davis, CA (United States). Dept. of Viticulture and Enology and Dept. of Plant Sciences
  3. Univ. of Georgia, Athens, GA (United States). Complex Carbohydrate Research Center
  4. Univ. of California, Davis, CA (United States). Dept. of Viticulture and Enology
Publication Date:
Research Org.:
Univ. of Georgia, Athens, GA (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Basic Energy Sciences (BES) (SC-22). Chemical Sciences, Geosciences & Biosciences Division
OSTI Identifier:
1523394
Grant/Contract Number:  
SC0015662
Resource Type:
Accepted Manuscript
Journal Name:
Nature Communications
Additional Journal Information:
Journal Volume: 9; Journal Issue: 1; Journal ID: ISSN 2041-1723
Publisher:
Nature Publishing Group
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES

Citation Formats

Rapicavoli, Jeannette N., Blanco-Ulate, Barbara, Muszyński, Artur, Figueroa-Balderas, Rosa, Morales-Cruz, Abraham, Azadi, Parastoo, Dobruchowska, Justyna M., Castro, Claudia, Cantu, Dario, and Roper, M. Caroline. Lipopolysaccharide O-antigen delays plant innate immune recognition of Xylella fastidiosa. United States: N. p., 2018. Web. doi:10.1038/s41467-018-02861-5.
Rapicavoli, Jeannette N., Blanco-Ulate, Barbara, Muszyński, Artur, Figueroa-Balderas, Rosa, Morales-Cruz, Abraham, Azadi, Parastoo, Dobruchowska, Justyna M., Castro, Claudia, Cantu, Dario, & Roper, M. Caroline. Lipopolysaccharide O-antigen delays plant innate immune recognition of Xylella fastidiosa. United States. doi:10.1038/s41467-018-02861-5.
Rapicavoli, Jeannette N., Blanco-Ulate, Barbara, Muszyński, Artur, Figueroa-Balderas, Rosa, Morales-Cruz, Abraham, Azadi, Parastoo, Dobruchowska, Justyna M., Castro, Claudia, Cantu, Dario, and Roper, M. Caroline. Fri . "Lipopolysaccharide O-antigen delays plant innate immune recognition of Xylella fastidiosa". United States. doi:10.1038/s41467-018-02861-5. https://www.osti.gov/servlets/purl/1523394.
@article{osti_1523394,
title = {Lipopolysaccharide O-antigen delays plant innate immune recognition of Xylella fastidiosa},
author = {Rapicavoli, Jeannette N. and Blanco-Ulate, Barbara and Muszyński, Artur and Figueroa-Balderas, Rosa and Morales-Cruz, Abraham and Azadi, Parastoo and Dobruchowska, Justyna M. and Castro, Claudia and Cantu, Dario and Roper, M. Caroline},
abstractNote = {Lipopolysaccharides (LPS) are among the known pathogen-associated molecular patterns (PAMPs). LPSs are potent elicitors of PAMP-triggered immunity (PTI), and bacteria have evolved intricate mechanisms to dampen PTI. Here we demonstrate that Xylella fastidiosa (Xf), a hemibiotrophic plant pathogenic bacterium, possesses a long chain O-antigen that enables it to delay initial plant recognition, thereby allowing it to effectively skirt initial elicitation of innate immunity and establish itself in the host. Lack of the O-antigen modifies plant perception of Xf and enables elicitation of hallmarks of PTI, such as ROS production specifically in the plant xylem tissue compartment, a tissue not traditionally considered a spatial location of PTI. To explore translational applications of our findings, we demonstrate that pre-treatment of plants with Xf LPS primes grapevine defenses to confer tolerance to Xf challenge.},
doi = {10.1038/s41467-018-02861-5},
journal = {Nature Communications},
number = 1,
volume = 9,
place = {United States},
year = {2018},
month = {1}
}

Journal Article:
Free Publicly Available Full Text
Publisher's Version of Record

Save / Share:

Works referenced in this record:

A simple and rapid method for the permethylation of carbohydrates
journal, August 1984