Sustainable bioproduction of the blue pigment indigoidine: Expanding the range of heterologous products in R. toruloides to include non-ribosomal peptides

Wehrs, Maren; Gladden, John M.; Liu, Yuzhong; Platz, Lukas; Prahl, Jan-Philip; Moon, Jadie; Papa, Gabriella; Sundstrom, Eric; Geiselman, Gina M.; Tanjore, Deepti; Keasling, Jay D.; Pray, Todd R.; Simmons, Blake A.; Mukhopadhyay, Aindrila

doi:10.1039/c9gc00920e

Title: Sustainable bioproduction of the blue pigment indigoidine: Expanding the range of heterologous products in R. toruloides to include non-ribosomal peptides

Journal Article · 28 May 2019 · Green Chemistry

DOI: https://doi.org/10.1039/c9gc00920e · OSTI ID:1518494

Wehrs, Maren ^[1];

^[2]; Liu, Yuzhong ^[3]; Platz, Lukas ^[3]; Prahl, Jan-Philip ^[3]; Moon, Jadie ^[3]; Papa, Gabriella ^[3]; Sundstrom, Eric ^[3]; Geiselman, Gina M. ^[4]; Tanjore, Deepti ^[3]; Keasling, Jay D. ^[5]; Pray, Todd R. ^[3];

^[3];

^[3]

Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States); Braunschweig Univ. of Technology (Germany); Joint BioEnergy Inst. (JBEI), Emeryville, CA (United States)
Joint BioEnergy Inst. (JBEI), Emeryville, CA (United States); Sandia National Lab. (SNL-CA), Livermore, CA (United States)
Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States); Joint BioEnergy Inst. (JBEI), Emeryville, CA (United States)
Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States); Sandia National Lab. (SNL-CA), Livermore, CA (United States)
Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States); Joint BioEnergy Inst. (JBEI), Emeryville, CA (United States); Univ. of California, Berkeley, CA (United States); Technical Univ. of Denmark, Lyngby (Denmark); Shenzhen Inst. for Advanced Technologies (China)

Non-ribosomal peptides (NRPs) constitute a diverse class of valuable secondary metabolites, with potential industrial applications including use as pharmaceuticals, polymers and dyes. Current industrial production of dyes is predominantly achieved via chemical synthesis, which can involve toxic precursors and generate hazardous byproducts. Thus, alternative routes of dye production are highly desirable to enhance both workplace safety and environmental sustainability. Similarly, biological synthesis of dyes from renewable carbon would serve an ideal green chemistry paradigm. Thus, we engineered the fungal host Rhodosporidium toruloides to produce the blue pigment indigoidine, an NRP with potential applications in the dye industry, using various low-cost carbon and nitrogen sources. To demonstrate production from renewable carbon sources and assess process scalability we produced indigoidine in 2 L bioreactors, reaching titers of 2.9 ± 0.8 g L^–1 using a sorghum lignocellulosic hydrolysate in a batch process and 86.3 ± 7.4 g L^–1 using glucose in a high-gravity fed-batch process. This study represents the first heterologous production of an NRP in R. toruloides, thus extending the range of microbial hosts that can be used for sustainable, heterologous production of NRPs. In addition, this is the first demonstration of producing an NRP using lignocellulose. These conclusions highlight the potential of R. toruloides for the sustainable, and scalable production of NRPs, with the highest reported titer of indigoidine or any heterologously produced NRP to date.

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Research Organization:: Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER); USDOE Office of Energy Efficiency and Renewable Energy (EERE), Office of Sustainable Transportation and Fuels. Bioenergy Technologies Office (BETO)

Grant/Contract Number:: AC02-05CH11231

OSTI ID:: 1518494

Alternate ID(s):: OSTI ID: 1542352

Journal Information:: Green Chemistry, Vol. 21, Issue 12; ISSN 1463-9262

Publisher:: Royal Society of ChemistryCopyright Statement

Country of Publication:: United States

Language:: English

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