Expression of a Lychee PHOSPHATIDYLCHOLINE:DIACYLGLYCEROL CHOLINEPHOSPHOTRANSFERASE with an E. coli CYCLOPROPANE SYNTHASE Enhances Cyclopropane Fatty Acid Accumulation in Camelina Seeds

Yu, Xiao-Hong; Cai, Yuanheng; Chai, Jin; Schwender, Jorg; Shanklin, John

doi:10.1104/pp.19.00396

Title: Expression of a Lychee PHOSPHATIDYLCHOLINE:DIACYLGLYCEROL CHOLINEPHOSPHOTRANSFERASE with an E. coli CYCLOPROPANE SYNTHASE Enhances Cyclopropane Fatty Acid Accumulation in Camelina Seeds

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Abstract

Cyclopropane fatty acids (CPA) are useful feedstocks for biofuels and bioproducts such as lubricants and biodiesel. Our goal is to pinpoint factors that can facilitate the accumulation of CPA in seed triacylglycerol, TAG (storage oil). We hypothesized that the poor metabolism of CPA through the TAG biosynthetic network could be overcome by addition of enzyme activities from species that naturally accumulate CPA in their seed oil, such as lychee (Lychi chinesis) which contains approximately 40% CPA in TAG. Our previous work on engineering CPA accumulation in crop and model plants identified a metabolic bottleneck between phosphatidylcholine (PC), the site of CPA biosynthesis, diacylglycerol (DAG) and TAG. In this work we report the cloning and heterologous expression in Camelina sativa of a lychee phosphatidylcholine:diacylglycerol cholinephosphotransferase (PDCT), which catalyzes the transfer of the phosphocholine headgroup from PC to DAG. Camelina sativa lines co-expressing LcPDCT and Escherichia coli cyclopropane synthase (EcCPS) showed up to 50% increase of CPA in mature seed, relative to the EcCPS background. Stereospecific lipid compositional analysis showed that the expression of LcPDCT strongly reduced the level of C18:1 substrate at PC-sn-1 and PC-sn-2 i.e., the sites of CPA synthesis, while the levels of CPA increased in PC-sn-2, DAG-sn-1 andmore »« less

Authors:: Yu, Xiao-Hong; Cai, Yuanheng; Chai, Jin; Schwender, Jorg; Shanklin, John

Publication Date:: Mon May 13 00:00:00 EDT 2019

Research Org.:: Brookhaven National Lab. (BNL), Upton, NY (United States); Center for Advanced Bioenergy and Bioproducts Innovation (CABBI), Urbana, IL (United States)

Sponsoring Org.:: USDOE Office of Science (SC), Biological and Environmental Research (BER); USDOE Office of Science (SC), Basic Energy Sciences (BES). Chemical Sciences, Geosciences, and Biosciences Division

OSTI Identifier:: 1515561

Alternate Identifier(s):: OSTI ID: 1512697; OSTI ID: 1546075

Report Number(s):: BNL-211611-2019-JAAM
Journal ID: ISSN 0032-0889; plantphysiol;pp.19.00396v1

Grant/Contract Number:: SC0012704; SC0018420

Resource Type:: Published Article

Journal Name:: Plant Physiology (Bethesda)

Additional Journal Information:: Journal Name: Plant Physiology (Bethesda); Journal ID: ISSN 0032-0889

Publisher:: American Society of Plant Biologists (ASPB)

Country of Publication:: United States

Language:: English

Subject:: 59 BASIC BIOLOGICAL SCIENCES; cyclopropane fatty acid; Camelina sativa; phosphatidylcholine diacylglycerol cholinephosphotransferase; lipid metabolism; triacylglycerol; 09 BIOMASS FUELS

Citation Formats


                    Yu, Xiao-Hong, Cai, Yuanheng, Chai, Jin, Schwender, Jorg, and Shanklin, John. Expression of a Lychee PHOSPHATIDYLCHOLINE:DIACYLGLYCEROL CHOLINEPHOSPHOTRANSFERASE with an E. coli CYCLOPROPANE SYNTHASE Enhances Cyclopropane Fatty Acid Accumulation in Camelina Seeds.  United States: N. p., 2019. 
Web.  doi:10.1104/pp.19.00396.

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                    Yu, Xiao-Hong, Cai, Yuanheng, Chai, Jin, Schwender, Jorg, & Shanklin, John. Expression of a Lychee PHOSPHATIDYLCHOLINE:DIACYLGLYCEROL CHOLINEPHOSPHOTRANSFERASE with an E. coli CYCLOPROPANE SYNTHASE Enhances Cyclopropane Fatty Acid Accumulation in Camelina Seeds.  United States.  https://doi.org/10.1104/pp.19.00396

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                    Yu, Xiao-Hong, Cai, Yuanheng, Chai, Jin, Schwender, Jorg, and Shanklin, John. Mon .  
"Expression of a Lychee PHOSPHATIDYLCHOLINE:DIACYLGLYCEROL CHOLINEPHOSPHOTRANSFERASE with an E. coli CYCLOPROPANE SYNTHASE Enhances Cyclopropane Fatty Acid Accumulation in Camelina Seeds".  United States.  https://doi.org/10.1104/pp.19.00396.

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@article{osti_1515561,

  title        = {Expression of a Lychee PHOSPHATIDYLCHOLINE:DIACYLGLYCEROL CHOLINEPHOSPHOTRANSFERASE with an E. coli CYCLOPROPANE SYNTHASE Enhances Cyclopropane Fatty Acid Accumulation in Camelina Seeds},

  author       = {Yu, Xiao-Hong and Cai, Yuanheng and Chai, Jin and Schwender, Jorg and Shanklin, John},

  abstractNote = {Cyclopropane fatty acids (CPA) are useful feedstocks for biofuels and bioproducts such as lubricants and biodiesel. Our goal is to pinpoint factors that can facilitate the accumulation of CPA in seed triacylglycerol, TAG (storage oil). We hypothesized that the poor metabolism of CPA through the TAG biosynthetic network could be overcome by addition of enzyme activities from species that naturally accumulate CPA in their seed oil, such as lychee (Lychi chinesis) which contains approximately 40% CPA in TAG. Our previous work on engineering CPA accumulation in crop and model plants identified a metabolic bottleneck between phosphatidylcholine (PC), the site of CPA biosynthesis, diacylglycerol (DAG) and TAG. In this work we report the cloning and heterologous expression in Camelina sativa of a lychee phosphatidylcholine:diacylglycerol cholinephosphotransferase (PDCT), which catalyzes the transfer of the phosphocholine headgroup from PC to DAG. Camelina sativa lines co-expressing LcPDCT and Escherichia coli cyclopropane synthase (EcCPS) showed up to 50% increase of CPA in mature seed, relative to the EcCPS background. Stereospecific lipid compositional analysis showed that the expression of LcPDCT strongly reduced the level of C18:1 substrate at PC-sn-1 and PC-sn-2 i.e., the sites of CPA synthesis, while the levels of CPA increased in PC-sn-2, DAG-sn-1 and DAG-sn-2 and both sn-1/ 3 and sn-2 positions in TAG. Taken together these data suggest that addition of PDCT activity facilitates more efficient movement of CPA from PC to DAG and establishes LcPDCT as a useful factor to combine with others to enhance CPA accumulation in plant seed oil.},

  doi          = {10.1104/pp.19.00396},

  journal      = {Plant Physiology (Bethesda)},

  number       = ,

  volume       = ,

  place        = {United States},

  year         = {Mon May 13 00:00:00 EDT 2019},

  month        = {Mon May 13 00:00:00 EDT 2019}

}

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