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Title: Hair Proteome Variation at Different Body Locations on Genetically Variant Peptide Detection for Protein-Based Human Identification

Abstract

Human hair contains minimal intact nuclear DNA for human identification in forensic and archaeological applications. In contrast, proteins offer a pathway to exploit hair evidence for human identification owing to their persistence, abundance, and derivation from DNA. Individualizing single nucleotide polymorphisms (SNPs) are often conserved as single amino acid polymorphisms in genetically variant peptides (GVPs). Detection of GVP markers in the hair proteome via high-resolution tandem mass spectrometry permits inference of SNPs with known statistical probabilities. To adopt this approach for forensic investigations, hair proteomic variation and its effects on GVP identification must first be characterized. This research aimed to assess variation in single-inch head, arm, and pubic hair, and discover body location-invariant GVP markers to distinguish individuals. Comparison of protein profiles revealed greater body location-specific variation in keratin-associated proteins and intracellular proteins, allowing body location differentiation. However, robust GVP markers derive primarily from keratins that do not exhibit body location-specific differential expression, supporting GVP identification independence from hair proteomic variation at the various body locations. Further, pairwise comparisons of GVP profiles with 8 SNPs demonstrated greatest interindividual variation and high intraindividual consistency, enabling similar differentiative potential of individuals using single hairs irrespective of body location origin.

Authors:
 [1];  [2]; ORCiD logo [2]; ORCiD logo [3];  [2]
  1. Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States). Forensic Science Center; Michigan State Univ., East Lansing, MI (United States). Dept. of Chemistry
  2. Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States). Forensic Science Center
  3. Michigan State Univ., East Lansing, MI (United States). Dept. of Chemistry. Dept. of Biochemistry and Molecular Biology
Publication Date:
Research Org.:
Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)
Sponsoring Org.:
USDOE; LLNL Laboratory Directed Research and Development (LDRD) Program
OSTI Identifier:
1515356
Report Number(s):
LLNL-JRNL-757511
Journal ID: ISSN 2045-2322; 945509
Grant/Contract Number:  
AC52-07NA27344
Resource Type:
Accepted Manuscript
Journal Name:
Scientific Reports
Additional Journal Information:
Journal Volume: 9; Journal ID: ISSN 2045-2322
Publisher:
Nature Publishing Group
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; genetic markers; proteomics

Citation Formats

Chu, Fanny, Mason, Katelyn E., Anex, Deon S., Jones, A. Daniel, and Hart, Bradley R. Hair Proteome Variation at Different Body Locations on Genetically Variant Peptide Detection for Protein-Based Human Identification. United States: N. p., 2019. Web. doi:10.1038/s41598-019-44007-7.
Chu, Fanny, Mason, Katelyn E., Anex, Deon S., Jones, A. Daniel, & Hart, Bradley R. Hair Proteome Variation at Different Body Locations on Genetically Variant Peptide Detection for Protein-Based Human Identification. United States. doi:10.1038/s41598-019-44007-7.
Chu, Fanny, Mason, Katelyn E., Anex, Deon S., Jones, A. Daniel, and Hart, Bradley R. Tue . "Hair Proteome Variation at Different Body Locations on Genetically Variant Peptide Detection for Protein-Based Human Identification". United States. doi:10.1038/s41598-019-44007-7. https://www.osti.gov/servlets/purl/1515356.
@article{osti_1515356,
title = {Hair Proteome Variation at Different Body Locations on Genetically Variant Peptide Detection for Protein-Based Human Identification},
author = {Chu, Fanny and Mason, Katelyn E. and Anex, Deon S. and Jones, A. Daniel and Hart, Bradley R.},
abstractNote = {Human hair contains minimal intact nuclear DNA for human identification in forensic and archaeological applications. In contrast, proteins offer a pathway to exploit hair evidence for human identification owing to their persistence, abundance, and derivation from DNA. Individualizing single nucleotide polymorphisms (SNPs) are often conserved as single amino acid polymorphisms in genetically variant peptides (GVPs). Detection of GVP markers in the hair proteome via high-resolution tandem mass spectrometry permits inference of SNPs with known statistical probabilities. To adopt this approach for forensic investigations, hair proteomic variation and its effects on GVP identification must first be characterized. This research aimed to assess variation in single-inch head, arm, and pubic hair, and discover body location-invariant GVP markers to distinguish individuals. Comparison of protein profiles revealed greater body location-specific variation in keratin-associated proteins and intracellular proteins, allowing body location differentiation. However, robust GVP markers derive primarily from keratins that do not exhibit body location-specific differential expression, supporting GVP identification independence from hair proteomic variation at the various body locations. Further, pairwise comparisons of GVP profiles with 8 SNPs demonstrated greatest interindividual variation and high intraindividual consistency, enabling similar differentiative potential of individuals using single hairs irrespective of body location origin.},
doi = {10.1038/s41598-019-44007-7},
journal = {Scientific Reports},
number = ,
volume = 9,
place = {United States},
year = {2019},
month = {5}
}

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