Hair Proteome Variation at Different Body Locations on Genetically Variant Peptide Detection for Protein-Based Human Identification

Chu, Fanny; Mason, Katelyn E.; Anex, Deon S.; Jones, A. Daniel; Hart, Bradley R.

doi:10.1038/s41598-019-44007-7

Title: Hair Proteome Variation at Different Body Locations on Genetically Variant Peptide Detection for Protein-Based Human Identification

Abstract

Abstract Human hair contains minimal intact nuclear DNA for human identification in forensic and archaeological applications. In contrast, proteins offer a pathway to exploit hair evidence for human identification owing to their persistence, abundance, and derivation from DNA. Individualizing single nucleotide polymorphisms (SNPs) are often conserved as single amino acid polymorphisms in genetically variant peptides (GVPs). Detection of GVP markers in the hair proteome via high-resolution tandem mass spectrometry permits inference of SNPs with known statistical probabilities. To adopt this approach for forensic investigations, hair proteomic variation and its effects on GVP identification must first be characterized. This research aimed to assess variation in single-inch head, arm, and pubic hair, and discover body location-invariant GVP markers to distinguish individuals. Comparison of protein profiles revealed greater body location-specific variation in keratin-associated proteins and intracellular proteins, allowing body location differentiation. However, robust GVP markers derive primarily from keratins that do not exhibit body location-specific differential expression, supporting GVP identification independence from hair proteomic variation at the various body locations. Further, pairwise comparisons of GVP profiles with 8 SNPs demonstrated greatest interindividual variation and high intraindividual consistency, enabling similar differentiative potential of individuals using single hairs irrespective of body location origin.

Authors:

Chu, Fanny; Mason, Katelyn E.;

;

; Hart, Bradley R.

Publication Date:: Tue May 21 00:00:00 EDT 2019

Research Org.:: Lawrence Livermore National Laboratory (LLNL), Livermore, CA (United States)

Sponsoring Org.:: USDOE; LLNL Laboratory Directed Research and Development (LDRD) Program

OSTI Identifier:: 1619638

Alternate Identifier(s):: OSTI ID: 1515356

Report Number(s):: LLNL-JRNL-757511
Journal ID: ISSN 2045-2322; 7641; PII: 44007

Grant/Contract Number:: AC52-07NA27344

Resource Type:: Published Article

Journal Name:: Scientific Reports

Additional Journal Information:: Journal Name: Scientific Reports Journal Volume: 9 Journal Issue: 1; Journal ID: ISSN 2045-2322

Publisher:: Nature Publishing Group

Country of Publication:: United Kingdom

Language:: English

Subject:: 59 BASIC BIOLOGICAL SCIENCES; genetic markers; proteomics

Citation Formats


                    Chu, Fanny, Mason, Katelyn E., Anex, Deon S., Jones, A. Daniel, and Hart, Bradley R. Hair Proteome Variation at Different Body Locations on Genetically Variant Peptide Detection for Protein-Based Human Identification.  United Kingdom: N. p., 2019. 
Web.  doi:10.1038/s41598-019-44007-7.

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                    Chu, Fanny, Mason, Katelyn E., Anex, Deon S., Jones, A. Daniel, & Hart, Bradley R. Hair Proteome Variation at Different Body Locations on Genetically Variant Peptide Detection for Protein-Based Human Identification.  United Kingdom.  https://doi.org/10.1038/s41598-019-44007-7

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                    Chu, Fanny, Mason, Katelyn E., Anex, Deon S., Jones, A. Daniel, and Hart, Bradley R. Tue .  
"Hair Proteome Variation at Different Body Locations on Genetically Variant Peptide Detection for Protein-Based Human Identification".  United Kingdom.  https://doi.org/10.1038/s41598-019-44007-7.

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@article{osti_1619638,

  title        = {Hair Proteome Variation at Different Body Locations on Genetically Variant Peptide Detection for Protein-Based Human Identification},

  author       = {Chu, Fanny and Mason, Katelyn E. and Anex, Deon S. and Jones, A. Daniel and Hart, Bradley R.},

  abstractNote = {Abstract Human hair contains minimal intact nuclear DNA for human identification in forensic and archaeological applications. In contrast, proteins offer a pathway to exploit hair evidence for human identification owing to their persistence, abundance, and derivation from DNA. Individualizing single nucleotide polymorphisms (SNPs) are often conserved as single amino acid polymorphisms in genetically variant peptides (GVPs). Detection of GVP markers in the hair proteome via high-resolution tandem mass spectrometry permits inference of SNPs with known statistical probabilities. To adopt this approach for forensic investigations, hair proteomic variation and its effects on GVP identification must first be characterized. This research aimed to assess variation in single-inch head, arm, and pubic hair, and discover body location-invariant GVP markers to distinguish individuals. Comparison of protein profiles revealed greater body location-specific variation in keratin-associated proteins and intracellular proteins, allowing body location differentiation. However, robust GVP markers derive primarily from keratins that do not exhibit body location-specific differential expression, supporting GVP identification independence from hair proteomic variation at the various body locations. Further, pairwise comparisons of GVP profiles with 8 SNPs demonstrated greatest interindividual variation and high intraindividual consistency, enabling similar differentiative potential of individuals using single hairs irrespective of body location origin.},

  doi          = {10.1038/s41598-019-44007-7},

  journal      = {Scientific Reports},

  number       = 1,

  volume       = 9,

  place        = {United Kingdom},

  year         = {Tue May 21 00:00:00 EDT 2019},

  month        = {Tue May 21 00:00:00 EDT 2019}

}

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https://doi.org/10.1038/s41598-019-44007-7

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Cited by: 16 works

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Figures / Tables:

Figure 1: Comparison of numbers of identified (a) proteins, (b) unique peptides, (c) amino acids, and missense SNPs inferred from (d) major and (e) minor GVPs at different body locations. Black lines represent statistically significant comparisons and significance levels are represented as: p ≤ 0.05 (*), p ≤ 0.01 (**),more »

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Works referenced in this record:

Human hair: A unique physicochemical composite
journal, June 2003

Wolfram, Leszek J.
Journal of the American Academy of Dermatology, Vol. 48, Issue 6
DOI: 10.1067/mjd.2003.276

The degradation of nucleic acids and montmorillonite-nucleic-acid complexes by soil microorganisms
journal, November 1970

Greaves, M. P.; Wilson, M. J.
Soil Biology and Biochemistry, Vol. 2, Issue 4
DOI: 10.1016/0038-0717(70)90032-5

Repeatability and Reproducibility in Proteomic Identifications by Liquid Chromatography−Tandem Mass Spectrometry
journal, February 2010

Tabb, David L.; Vega-Montoto, Lorenzo; Rudnick, Paul A.
Journal of Proteome Research, Vol. 9, Issue 2
DOI: 10.1021/pr9006365

Characterisation of novel α-keratin peptide markers for species identification in keratinous tissues using mass spectrometry: Novel α-keratin peptide markers for species identification
journal, October 2013

Solazzo, Caroline; Wadsley, Marc; Dyer, Jolon M.
Rapid Communications in Mass Spectrometry, Vol. 27, Issue 23
DOI: 10.1002/rcm.6730

Human Hair Keratin-Associated Proteins
journal, December 2005

Shimomura, Yutaka; Ito, Masaaki
Journal of Investigative Dermatology Symposium Proceedings, Vol. 10, Issue 3
DOI: 10.1111/j.1087-0024.2005.10112.x

Stabilization of Solutions of Feather Keratins by Sodium Dodecyl Sulfate
journal, August 2001

Schrooyen, Peter M. M.; Dijkstra, Pieter J.; Oberthür, Radulf C.
Journal of Colloid and Interface Science, Vol. 240, Issue 1
DOI: 10.1006/jcis.2001.7673

Label-free, normalized quantification of complex mass spectrometry data for proteomic analysis
journal, December 2009

Griffin, Noelle M.; Yu, Jingyi; Long, Fred
Nature Biotechnology, Vol. 28, Issue 1
DOI: 10.1038/nbt.1592

Analysis of protein-coding genetic variation in 60,706 humans
journal, August 2016

Lek, Monkol; Karczewski, Konrad J.; Minikel, Eric V.
Nature, Vol. 536, Issue 7616
DOI: 10.1038/nature19057

Effect of shampoo, conditioner and permanent waving on the molecular structure of human hair
journal, January 2015

Zhang, Yuchen; Alsop, Richard J.; Soomro, Asfia
PeerJ, Vol. 3
DOI: 10.7717/peerj.1296

Short tandem repeat (STR) genotyping of keratinised hair Part 2. An optimised genomic DNA extraction procedure reveals donor dependence of STR profiles
journal, October 2005

McNevin, Dennis; Wilson-Wilde, Linzi; Robertson, James
Forensic Science International, Vol. 153, Issue 2-3
DOI: 10.1016/j.forsciint.2005.05.005

Identification of Antibacterial Components in Human Hair Shafts
journal, January 2018

Subbaiah, R.; Kerk, S.; Lian, Y.
Acta Dermato Venereologica, Vol. 98, Issue 7
DOI: 10.2340/00015555-2952

Development of SNP-based human identification system
journal, November 2009

Kim, Jae-Jung; Han, Bok-Ghee; Lee, Hae-In
International Journal of Legal Medicine, Vol. 124, Issue 2
DOI: 10.1007/s00414-009-0389-9

Quantification of Proteins and Metabolites by Mass Spectrometry without Isotopic Labeling or Spiked Standards
journal, September 2003

Wang, Weixun; Zhou, Haihong; Lin, Hua
Analytical Chemistry, Vol. 75, Issue 18
DOI: 10.1021/ac026468x

Effects of DNA Polymer Length on Its Adsorption to Soils
journal, January 1994

Ogram, Andrew V.; Mathot, Mark L.; Harsh, James B.
Applied and Environmental Microbiology, Vol. 60, Issue 2
DOI: 10.1128/aem.60.2.393-396.1994

A Model for Random Sampling and Estimation of Relative Protein Abundance in Shotgun Proteomics
journal, July 2004

Liu, Hongbin; Sadygov, Rovshan G.; Yates, John R.
Analytical Chemistry, Vol. 76, Issue 14
DOI: 10.1021/ac0498563

A Review of Keratin-Based Biomaterials for Biomedical Applications
journal, February 2010

Rouse, Jillian G.; Van Dyke, Mark E.
Materials, Vol. 3, Issue 2
DOI: 10.3390/ma3020999

Haplotype Variation and Linkage Disequilibrium in 313 Human Genes
journal, July 2001

Stephens, J. C.
Science, Vol. 293, Issue 5529
DOI: 10.1126/science.1059431

Developing a SNP panel for forensic identification of individuals
journal, December 2006

Kidd, Kenneth K.; Pakstis, Andrew J.; Speed, William C.
Forensic Science International, Vol. 164, Issue 1
DOI: 10.1016/j.forsciint.2005.11.017

Proteome degradation in fossils: investigating the longevity of protein survival in ancient bone: Proteome degradation in fossils
journal, February 2014

Wadsworth, Caroline; Buckley, Mike
Rapid Communications in Mass Spectrometry, Vol. 28, Issue 6
DOI: 10.1002/rcm.6821

Proteomic analysis of hair shafts from monozygotic twins: Expression profiles and genetically variant peptides
journal, June 2017

Wu, Pei-Wen; Mason, Katelyn E.; Durbin-Johnson, Blythe P.
PROTEOMICS, Vol. 17, Issue 13-14
DOI: 10.1002/pmic.201600462

Forensic DNA typing by capillary electrophoresis using the ABI Prism 310 and 3100 genetic analyzers for STR analysis
journal, June 2004

Butler, John M.; Buel, Eric; Crivellente, Federica
ELECTROPHORESIS, Vol. 25, Issue 1011
DOI: 10.1002/elps.200305822

Cuticle cell defects in lamellar ichthyosis hair and anomalous hair shaft syndromes visualized after detergent extraction
journal, December 1996

Rice, Robert H.; Wong, Viviana J.; Price, Vera H.
The Anatomical Record, Vol. 246, Issue 4
DOI: 10.1002/(SICI)1097-0185(199612)246:4<433::AID-AR2>3.0.CO;2-W

Linkage disequilibrium in the human genome
journal, May 2001

Reich, David E.; Cargill, Michele; Bolk, Stacey
Nature, Vol. 411, Issue 6834
DOI: 10.1038/35075590

Effect of Dynamic Exclusion Duration on Spectral Count Based Quantitative Proteomics
journal, August 2009

Zhang, Ying; Wen, Zhihui; Washburn, Michael P.
Analytical Chemistry, Vol. 81, Issue 15
DOI: 10.1021/ac9004887

Quantitative Profiling of Proteins in Complex Mixtures Using Liquid Chromatography and Mass Spectrometry
journal, August 2002

Chelius, Dirk; Bondarenko, Pavel V.
Journal of Proteome Research, Vol. 1, Issue 4
DOI: 10.1021/pr025517j

In situ labeling of DNA reveals interindividual variation in nuclear DNA breakdown in hair and may be useful to predict success of forensic genotyping of hair
journal, April 2011

Szabo, Sandra; Jaeger, Karin; Fischer, Heinz
International Journal of Legal Medicine, Vol. 126, Issue 1
DOI: 10.1007/s00414-011-0566-5

Demonstration of Protein-Based Human Identification Using the Hair Shaft Proteome
journal, September 2016

Parker, Glendon J.; Leppert, Tami; Anex, Deon S.
PLOS ONE, Vol. 11, Issue 9
DOI: 10.1371/journal.pone.0160653

Studies on the Proteome of Human Hair - Identification of Histones and Deamidated Keratins
journal, January 2018

Adav, Sunil S.; Subbaiaih, Roopa S.; Kerk, Swat Kim
Scientific Reports, Vol. 8, Issue 1
DOI: 10.1038/s41598-018-20041-9

Human hair shaft proteomic profiling: individual differences, site specificity and cuticle analysis
journal, January 2014

Laatsch, Chelsea N.; Durbin-Johnson, Blythe P.; Rocke, David M.
PeerJ, Vol. 2
DOI: 10.7717/peerj.506

The Catalog of Human Hair Keratins: I. EXPRESSION OF THE NINE TYPE I MEMBERS IN THE HAIR FOLLICLE
journal, July 1999

Langbein, Lutz; Rogers, Michael A.; Winter, Hermelita
Journal of Biological Chemistry, Vol. 274, Issue 28
DOI: 10.1074/jbc.274.28.19874

Proteome Analysis of Human Hair Shaft: From Protein Identification to Posttranslational Modification
journal, January 2006

Lee, Young Jin; Rice, Robert H.; Lee, Young Moo
Molecular & Cellular Proteomics, Vol. 5, Issue 5
DOI: 10.1074/mcp.M500278-MCP200

Development of a Protein‐based Human Identification Capability from a Single Hair
journal, February 2019

Mason, Katelyn E.; Paul, Phillip H.; Chu, Fanny
Journal of Forensic Sciences, Vol. 64, Issue 4
DOI: 10.1111/1556-4029.13995

Hair follicle-specific keratins and their diseases
journal, June 2007

Schweizer, Jürgen; Langbein, Lutz; Rogers, Michael A.
Experimental Cell Research, Vol. 313, Issue 10
DOI: 10.1016/j.yexcr.2007.02.032

The Catalog of Human Hair Keratins: II. EXPRESSION OF THE SIX TYPE II MEMBERS IN THE HAIR FOLLICLE AND THE COMBINED CATALOG OF HUMAN TYPE I AND II KERATINS
journal, July 2001

Langbein, Lutz; Rogers, Michael A.; Winter, Hermelita
Journal of Biological Chemistry, Vol. 276, Issue 37
DOI: 10.1074/jbc.M103305200

Discovery of highly specific protein markers for the identification of biological stains: Proteomics and 2DE
journal, October 2014

Legg, Kevin M.; Powell, Roger; Reisdorph, Nichole
ELECTROPHORESIS, Vol. 35, Issue 21-22
DOI: 10.1002/elps.201400125

SNPs for a universal individual identification panel
journal, November 2009

Pakstis, Andrew J.; Speed, William C.; Fang, Rixun
Human Genetics, Vol. 127, Issue 3
DOI: 10.1007/s00439-009-0771-1

Verification of protein biomarker specificity for the identification of biological stains by quadrupole time-of-flight mass spectrometry: Proteomics and 2-DE
journal, January 2017

Legg, Kevin M.; Powell, Roger; Reisdorph, Nichole
ELECTROPHORESIS, Vol. 38, Issue 6
DOI: 10.1002/elps.201600352

Figures / Tables found in this record:

Supplementary Figure S1 (p. 38)

Supplementary Figure S2 (p. 39)

Supplementary Figure S3 (p. 40)

Supplementary Figure S4 (p. 41)

Supplementary Figure S5 (p. 42)

Supplementary Table S1 (p. 43)

Supplementary Table S1 (cont) (p. 44)

Supplementary Table S1 (cont) (p. 45)

Supplementary Table S1 (cont) (p. 46)

Supplementary Table S1 (cont) (p. 47)

Supplementary Table S1 (cont) (p. 48)

Supplementary Table S1 (cont) (p. 49)

Supplementary Table S1 (cont) (p. 50)

Supplementary Table S1 (cont) (p. 51)

Supplementary Table S1 (cont) (p. 52)

Supplementary Table S1 (cont) (p. 53)

Supplementary Table S1 (cont) (p. 54)

Figures/Tables have been extracted from DOE-funded journal article accepted manuscripts.

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Title: Hair Proteome Variation at Different Body Locations on Genetically Variant Peptide Detection for Protein-Based Human Identification

Abstract

Citation Formats

Figures / Tables:

Human hair: A unique physicochemical composite journal, June 2003

The degradation of nucleic acids and montmorillonite-nucleic-acid complexes by soil microorganisms journal, November 1970

Repeatability and Reproducibility in Proteomic Identifications by Liquid Chromatography−Tandem Mass Spectrometry journal, February 2010

Characterisation of novel α-keratin peptide markers for species identification in keratinous tissues using mass spectrometry: Novel α-keratin peptide markers for species identification journal, October 2013

Human Hair Keratin-Associated Proteins journal, December 2005

Stabilization of Solutions of Feather Keratins by Sodium Dodecyl Sulfate journal, August 2001

Label-free, normalized quantification of complex mass spectrometry data for proteomic analysis journal, December 2009

Analysis of protein-coding genetic variation in 60,706 humans journal, August 2016

Effect of shampoo, conditioner and permanent waving on the molecular structure of human hair journal, January 2015

Short tandem repeat (STR) genotyping of keratinised hair Part 2. An optimised genomic DNA extraction procedure reveals donor dependence of STR profiles journal, October 2005

Identification of Antibacterial Components in Human Hair Shafts journal, January 2018

Development of SNP-based human identification system journal, November 2009

Quantification of Proteins and Metabolites by Mass Spectrometry without Isotopic Labeling or Spiked Standards journal, September 2003

Effects of DNA Polymer Length on Its Adsorption to Soils journal, January 1994

A Model for Random Sampling and Estimation of Relative Protein Abundance in Shotgun Proteomics journal, July 2004

A Review of Keratin-Based Biomaterials for Biomedical Applications journal, February 2010

Haplotype Variation and Linkage Disequilibrium in 313 Human Genes journal, July 2001

Developing a SNP panel for forensic identification of individuals journal, December 2006

Proteome degradation in fossils: investigating the longevity of protein survival in ancient bone: Proteome degradation in fossils journal, February 2014

Proteomic analysis of hair shafts from monozygotic twins: Expression profiles and genetically variant peptides journal, June 2017

Forensic DNA typing by capillary electrophoresis using the ABI Prism 310 and 3100 genetic analyzers for STR analysis journal, June 2004

Cuticle cell defects in lamellar ichthyosis hair and anomalous hair shaft syndromes visualized after detergent extraction journal, December 1996

Linkage disequilibrium in the human genome journal, May 2001

Effect of Dynamic Exclusion Duration on Spectral Count Based Quantitative Proteomics journal, August 2009

Quantitative Profiling of Proteins in Complex Mixtures Using Liquid Chromatography and Mass Spectrometry journal, August 2002

In situ labeling of DNA reveals interindividual variation in nuclear DNA breakdown in hair and may be useful to predict success of forensic genotyping of hair journal, April 2011

Demonstration of Protein-Based Human Identification Using the Hair Shaft Proteome journal, September 2016

Studies on the Proteome of Human Hair - Identification of Histones and Deamidated Keratins journal, January 2018

Human hair shaft proteomic profiling: individual differences, site specificity and cuticle analysis journal, January 2014

The Catalog of Human Hair Keratins: I. EXPRESSION OF THE NINE TYPE I MEMBERS IN THE HAIR FOLLICLE journal, July 1999

Proteome Analysis of Human Hair Shaft: From Protein Identification to Posttranslational Modification journal, January 2006

Development of a Protein‐based Human Identification Capability from a Single Hair journal, February 2019

Hair follicle-specific keratins and their diseases journal, June 2007

The Catalog of Human Hair Keratins: II. EXPRESSION OF THE SIX TYPE II MEMBERS IN THE HAIR FOLLICLE AND THE COMBINED CATALOG OF HUMAN TYPE I AND II KERATINS journal, July 2001

Discovery of highly specific protein markers for the identification of biological stains: Proteomics and 2DE journal, October 2014

SNPs for a universal individual identification panel journal, November 2009

Verification of protein biomarker specificity for the identification of biological stains by quadrupole time-of-flight mass spectrometry: Proteomics and 2-DE journal, January 2017

Human hair: A unique physicochemical composite
journal, June 2003

The degradation of nucleic acids and montmorillonite-nucleic-acid complexes by soil microorganisms
journal, November 1970

Repeatability and Reproducibility in Proteomic Identifications by Liquid Chromatography−Tandem Mass Spectrometry
journal, February 2010

Characterisation of novel α-keratin peptide markers for species identification in keratinous tissues using mass spectrometry: Novel α-keratin peptide markers for species identification
journal, October 2013

Human Hair Keratin-Associated Proteins
journal, December 2005

Stabilization of Solutions of Feather Keratins by Sodium Dodecyl Sulfate
journal, August 2001

Label-free, normalized quantification of complex mass spectrometry data for proteomic analysis
journal, December 2009

Analysis of protein-coding genetic variation in 60,706 humans
journal, August 2016

Effect of shampoo, conditioner and permanent waving on the molecular structure of human hair
journal, January 2015

Short tandem repeat (STR) genotyping of keratinised hair Part 2. An optimised genomic DNA extraction procedure reveals donor dependence of STR profiles
journal, October 2005

Identification of Antibacterial Components in Human Hair Shafts
journal, January 2018

Development of SNP-based human identification system
journal, November 2009

Quantification of Proteins and Metabolites by Mass Spectrometry without Isotopic Labeling or Spiked Standards
journal, September 2003

Effects of DNA Polymer Length on Its Adsorption to Soils
journal, January 1994

A Model for Random Sampling and Estimation of Relative Protein Abundance in Shotgun Proteomics
journal, July 2004

A Review of Keratin-Based Biomaterials for Biomedical Applications
journal, February 2010

Haplotype Variation and Linkage Disequilibrium in 313 Human Genes
journal, July 2001

Developing a SNP panel for forensic identification of individuals
journal, December 2006

Proteome degradation in fossils: investigating the longevity of protein survival in ancient bone: Proteome degradation in fossils
journal, February 2014

Proteomic analysis of hair shafts from monozygotic twins: Expression profiles and genetically variant peptides
journal, June 2017

Forensic DNA typing by capillary electrophoresis using the ABI Prism 310 and 3100 genetic analyzers for STR analysis
journal, June 2004

Cuticle cell defects in lamellar ichthyosis hair and anomalous hair shaft syndromes visualized after detergent extraction
journal, December 1996

Linkage disequilibrium in the human genome
journal, May 2001

Effect of Dynamic Exclusion Duration on Spectral Count Based Quantitative Proteomics
journal, August 2009

Quantitative Profiling of Proteins in Complex Mixtures Using Liquid Chromatography and Mass Spectrometry
journal, August 2002

In situ labeling of DNA reveals interindividual variation in nuclear DNA breakdown in hair and may be useful to predict success of forensic genotyping of hair
journal, April 2011

Demonstration of Protein-Based Human Identification Using the Hair Shaft Proteome
journal, September 2016

Studies on the Proteome of Human Hair - Identification of Histones and Deamidated Keratins
journal, January 2018

Human hair shaft proteomic profiling: individual differences, site specificity and cuticle analysis
journal, January 2014

The Catalog of Human Hair Keratins: I. EXPRESSION OF THE NINE TYPE I MEMBERS IN THE HAIR FOLLICLE
journal, July 1999

Proteome Analysis of Human Hair Shaft: From Protein Identification to Posttranslational Modification
journal, January 2006

Development of a Protein‐based Human Identification Capability from a Single Hair
journal, February 2019

Hair follicle-specific keratins and their diseases
journal, June 2007

The Catalog of Human Hair Keratins: II. EXPRESSION OF THE SIX TYPE II MEMBERS IN THE HAIR FOLLICLE AND THE COMBINED CATALOG OF HUMAN TYPE I AND II KERATINS
journal, July 2001

Discovery of highly specific protein markers for the identification of biological stains: Proteomics and 2DE
journal, October 2014

SNPs for a universal individual identification panel
journal, November 2009

Verification of protein biomarker specificity for the identification of biological stains by quadrupole time-of-flight mass spectrometry: Proteomics and 2-DE
journal, January 2017