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Title: Kinesin motor density and dynamics in gliding microtubule motility

Abstract

Kinesin motors and their associated filaments, microtubules, are essential to many biological processes. The motor and filament system can be reconstituted in vitro with the surface-adhered motors transporting the filaments along the surface. In this format, the system has been used to study active self-assembly and to power microdevices or perform analyte detection. Yet, fundamental properties of the system, such as the spacing of the kinesin motors bound to the microtubule and the dynamics of binding, remain poorly understood. We show that Fluorescence Interference Contrast (FLIC) microscopy can illuminate the exact height of the microtubule, which for a sufficiently low surface density of kinesin, reveals the locations of the bound motors. We investigate the spacing of the kinesin motors on the microtubules at various kinesin surface densities and compare the results with theory. FLIC reveals that the system is highly dynamic, with kinesin binding and unbinding along the length of the microtubule as it is transported along the surface.

Authors:
 [1];  [1];  [1]
  1. Sandia National Lab. (SNL-NM), Albuquerque, NM (United States)
Publication Date:
Research Org.:
Sandia National Lab. (SNL-NM), Albuquerque, NM (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Basic Energy Sciences (BES) (SC-22)
OSTI Identifier:
1515204
Report Number(s):
SAND-2019-5346J
Journal ID: ISSN 2045-2322; 675469
Grant/Contract Number:  
AC04-94AL85000
Resource Type:
Accepted Manuscript
Journal Name:
Scientific Reports
Additional Journal Information:
Journal Volume: 9; Journal Issue: 1; Journal ID: ISSN 2045-2322
Publisher:
Nature Publishing Group
Country of Publication:
United States
Language:
English
Subject:
77 NANOSCIENCE AND NANOTECHNOLOGY

Citation Formats

VanDelinder, Virginia, Imam, Zachary I., and Bachand, George. Kinesin motor density and dynamics in gliding microtubule motility. United States: N. p., 2019. Web. doi:10.1038/s41598-019-43749-8.
VanDelinder, Virginia, Imam, Zachary I., & Bachand, George. Kinesin motor density and dynamics in gliding microtubule motility. United States. doi:10.1038/s41598-019-43749-8.
VanDelinder, Virginia, Imam, Zachary I., and Bachand, George. Fri . "Kinesin motor density and dynamics in gliding microtubule motility". United States. doi:10.1038/s41598-019-43749-8. https://www.osti.gov/servlets/purl/1515204.
@article{osti_1515204,
title = {Kinesin motor density and dynamics in gliding microtubule motility},
author = {VanDelinder, Virginia and Imam, Zachary I. and Bachand, George},
abstractNote = {Kinesin motors and their associated filaments, microtubules, are essential to many biological processes. The motor and filament system can be reconstituted in vitro with the surface-adhered motors transporting the filaments along the surface. In this format, the system has been used to study active self-assembly and to power microdevices or perform analyte detection. Yet, fundamental properties of the system, such as the spacing of the kinesin motors bound to the microtubule and the dynamics of binding, remain poorly understood. We show that Fluorescence Interference Contrast (FLIC) microscopy can illuminate the exact height of the microtubule, which for a sufficiently low surface density of kinesin, reveals the locations of the bound motors. We investigate the spacing of the kinesin motors on the microtubules at various kinesin surface densities and compare the results with theory. FLIC reveals that the system is highly dynamic, with kinesin binding and unbinding along the length of the microtubule as it is transported along the surface.},
doi = {10.1038/s41598-019-43749-8},
journal = {Scientific Reports},
number = 1,
volume = 9,
place = {United States},
year = {2019},
month = {5}
}

Journal Article:
Free Publicly Available Full Text
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Cited by: 3 works
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Figures / Tables:

Figure 1 Figure 1: Cartoon (not to scale) of microtubule in gliding motility assay with high (A) and low (B) kinesin surface density. In the low-density example, the grey level of the microtubule corresponds to the relative fluorescence signal in FLIC microscopy.

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