Kinesin motor density and dynamics in gliding microtubule motility
Abstract
Kinesin motors and their associated filaments, microtubules, are essential to many biological processes. The motor and filament system can be reconstituted in vitro with the surface-adhered motors transporting the filaments along the surface. In this format, the system has been used to study active self-assembly and to power microdevices or perform analyte detection. Yet, fundamental properties of the system, such as the spacing of the kinesin motors bound to the microtubule and the dynamics of binding, remain poorly understood. We show that Fluorescence Interference Contrast (FLIC) microscopy can illuminate the exact height of the microtubule, which for a sufficiently low surface density of kinesin, reveals the locations of the bound motors. We investigate the spacing of the kinesin motors on the microtubules at various kinesin surface densities and compare the results with theory. FLIC reveals that the system is highly dynamic, with kinesin binding and unbinding along the length of the microtubule as it is transported along the surface.
- Authors:
- Publication Date:
- Research Org.:
- Sandia National Lab. (SNL-NM), Albuquerque, NM (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC), Basic Energy Sciences (BES)
- OSTI Identifier:
- 1619514
- Alternate Identifier(s):
- OSTI ID: 1515204
- Report Number(s):
- SAND-2019-5346J
Journal ID: ISSN 2045-2322; 7206; PII: 43749
- Grant/Contract Number:
- AC04-94AL85000
- Resource Type:
- Published Article
- Journal Name:
- Scientific Reports
- Additional Journal Information:
- Journal Name: Scientific Reports Journal Volume: 9 Journal Issue: 1; Journal ID: ISSN 2045-2322
- Publisher:
- Nature Publishing Group
- Country of Publication:
- United Kingdom
- Language:
- English
- Subject:
- 77 NANOSCIENCE AND NANOTECHNOLOGY
Citation Formats
VanDelinder, Virginia, Imam, Zachary I., and Bachand, George. Kinesin motor density and dynamics in gliding microtubule motility. United Kingdom: N. p., 2019.
Web. doi:10.1038/s41598-019-43749-8.
VanDelinder, Virginia, Imam, Zachary I., & Bachand, George. Kinesin motor density and dynamics in gliding microtubule motility. United Kingdom. https://doi.org/10.1038/s41598-019-43749-8
VanDelinder, Virginia, Imam, Zachary I., and Bachand, George. Fri .
"Kinesin motor density and dynamics in gliding microtubule motility". United Kingdom. https://doi.org/10.1038/s41598-019-43749-8.
@article{osti_1619514,
title = {Kinesin motor density and dynamics in gliding microtubule motility},
author = {VanDelinder, Virginia and Imam, Zachary I. and Bachand, George},
abstractNote = {Kinesin motors and their associated filaments, microtubules, are essential to many biological processes. The motor and filament system can be reconstituted in vitro with the surface-adhered motors transporting the filaments along the surface. In this format, the system has been used to study active self-assembly and to power microdevices or perform analyte detection. Yet, fundamental properties of the system, such as the spacing of the kinesin motors bound to the microtubule and the dynamics of binding, remain poorly understood. We show that Fluorescence Interference Contrast (FLIC) microscopy can illuminate the exact height of the microtubule, which for a sufficiently low surface density of kinesin, reveals the locations of the bound motors. We investigate the spacing of the kinesin motors on the microtubules at various kinesin surface densities and compare the results with theory. FLIC reveals that the system is highly dynamic, with kinesin binding and unbinding along the length of the microtubule as it is transported along the surface.},
doi = {10.1038/s41598-019-43749-8},
journal = {Scientific Reports},
number = 1,
volume = 9,
place = {United Kingdom},
year = {Fri May 10 00:00:00 EDT 2019},
month = {Fri May 10 00:00:00 EDT 2019}
}
https://doi.org/10.1038/s41598-019-43749-8
Web of Science
Figures / Tables:
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Figures / Tables found in this record: