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Title: Regulatory Tools for Controlling Gene Expression in Cyanobacteria

Abstract

Cyanobacteria are attractive hosts for converting carbon dioxide and sunlight into desirable chemical products. To engineer these organisms and manipulate their metabolic pathways, the biotechnology community has developed genetic tools to control gene expression. Many native cyanobacterial promoters and related sequence elements have been used to regulate genes of interest, and heterologous tools that use non-native small molecules to induce gene expression have been demonstrated. Overall, IPTG-based induction systems seem to be leaky and initially demonstrate small dynamic ranges in cyanobacteria. Consequently, a variety of other induction systems have been optimized to enable tighter control of gene expression. Tools require significant optimization because they function quite differently in cyanobacteria when compared to analogous use in model heterotrophs. We hypothesize that these differences are due to fundamental differences in physiology between organisms. This review is not intended to summarize all known products made in cyanobacteria nor the performance (titer, rate, yield) of individual strains, but instead will focus on the genetic tools and the inherent aspects of cellular physiology that influence gene expression in cyanobacteria.

Authors:
 [1];  [1]
  1. Univ. of Wisconsin, Madison, WI (United States). Dept. of Chemical and Biological Engineering, and Microbiology Doctoral Training Program
Publication Date:
Research Org.:
Univ. of Wisconsin, Madison, WI (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1515186
Grant/Contract Number:  
SC0010329
Resource Type:
Accepted Manuscript
Journal Name:
Advances in Experimental Medicine and Biology
Additional Journal Information:
Journal Volume: 1080; Journal ID: ISSN 0065-2598
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; Promoter; Induction system; Termination; Genome editing

Citation Formats

Gordon, Gina C., and Pfleger, Brian F. Regulatory Tools for Controlling Gene Expression in Cyanobacteria. United States: N. p., 2018. Web. doi:10.1007/978-981-13-0854-3_12.
Gordon, Gina C., & Pfleger, Brian F. Regulatory Tools for Controlling Gene Expression in Cyanobacteria. United States. doi:10.1007/978-981-13-0854-3_12.
Gordon, Gina C., and Pfleger, Brian F. Thu . "Regulatory Tools for Controlling Gene Expression in Cyanobacteria". United States. doi:10.1007/978-981-13-0854-3_12. https://www.osti.gov/servlets/purl/1515186.
@article{osti_1515186,
title = {Regulatory Tools for Controlling Gene Expression in Cyanobacteria},
author = {Gordon, Gina C. and Pfleger, Brian F.},
abstractNote = {Cyanobacteria are attractive hosts for converting carbon dioxide and sunlight into desirable chemical products. To engineer these organisms and manipulate their metabolic pathways, the biotechnology community has developed genetic tools to control gene expression. Many native cyanobacterial promoters and related sequence elements have been used to regulate genes of interest, and heterologous tools that use non-native small molecules to induce gene expression have been demonstrated. Overall, IPTG-based induction systems seem to be leaky and initially demonstrate small dynamic ranges in cyanobacteria. Consequently, a variety of other induction systems have been optimized to enable tighter control of gene expression. Tools require significant optimization because they function quite differently in cyanobacteria when compared to analogous use in model heterotrophs. We hypothesize that these differences are due to fundamental differences in physiology between organisms. This review is not intended to summarize all known products made in cyanobacteria nor the performance (titer, rate, yield) of individual strains, but instead will focus on the genetic tools and the inherent aspects of cellular physiology that influence gene expression in cyanobacteria.},
doi = {10.1007/978-981-13-0854-3_12},
journal = {Advances in Experimental Medicine and Biology},
number = ,
volume = 1080,
place = {United States},
year = {2018},
month = {8}
}

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journal, January 2012


Metabolic Engineering of Synechocystis sp. Strain PCC 6803 for Isobutanol Production
journal, November 2012

  • Varman, Arul M.; Xiao, Yi; Pakrasi, Himadri B.
  • Applied and Environmental Microbiology, Vol. 79, Issue 3
  • DOI: 10.1128/AEM.02827-12

CyAbrB2 Contributes to the Transcriptional Regulation of Low CO 2 Acclimation in Synechocystis sp. PCC 6803
journal, September 2016

  • Orf, Isabel; Schwarz, Doreen; Kaplan, Aaron
  • Plant and Cell Physiology, Vol. 57, Issue 10
  • DOI: 10.1093/pcp/pcw146