Antifouling Properties of a Self-Assembling Glutamic Acid-Lysine Zwitterionic Polymer Surface Coating
Abstract
There is a necessity for the development of antifouling materials to resist adsorption of biomacromolecules. Here we describe the preparation of a novel zwitterionic block copolymer with the potential to prevent or delay the formation of microbial biofilms. The block copolymer comprised a zwitterionic (hydrophilic) section of alternating glutamic acid (negatively charged) and lysine (positively charged) units and a hydrophobic polystyrene section. Cryo-TEM and dynamic light-scattering (DLS) results showed that, on average, the block copolymer self-assembled into 7-nm-diameter micelles in aqueous solutions (0 to 100 mM NaCl, pH 6). Quartz crystal microbalance with dissipation monitoring (QCM-D), atomic force microscopy (AFM), and contact angle measurements demonstrated that the block copolymer self-assembled into a brush-like monolayer on polystyrene surfaces. The brush-like monolayer produced from a 100 mg/L block copolymer solution exhibited an average distance, d, of approximately 4-8 nm between each block copolymer molecule (center to center). Once the brush-like monolayer self-assembled, it reduced EPS adsorption onto the polystyrene surface by similar to 70% (mass), reduced the rate of bacterial attachment by >80%, and inhibited the development of thick biofilms. QCM-D results revealed that the EPS molecules penetrate between the chains of the brush and adsorb onto the polystyrene surface. Additionally, AFMmore »
- Authors:
-
- Ben-Gurion University of the Negev (BGU), Beersheba (Israel)
- Univ. of Chicago, IL (United States)
- Univ. of Chicago, IL (United States); Argonne National Lab. (ANL), Lemont, IL (United States)
- Publication Date:
- Research Org.:
- Argonne National Lab. (ANL), Argonne, IL (United States)
- Sponsoring Org.:
- Ben-Gurion University of the Negev (BGU), Beersheba (Israel)
- OSTI Identifier:
- 1514874
- Grant/Contract Number:
- AC02-06CH11357
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Langmuir
- Additional Journal Information:
- Journal Volume: 35; Journal Issue: 5; Journal ID: ISSN 0743-7463
- Publisher:
- American Chemical Society
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY
Citation Formats
Ziemba, Christopher, Khavkin, Maria, Priftis, Dimitris, Acar, Handan, Mao, Jun, Benami, Maya, Gottlieb, Moshe, Tirrell, Matthew, Kaufman, Yair, and Herzberg, Moshe. Antifouling Properties of a Self-Assembling Glutamic Acid-Lysine Zwitterionic Polymer Surface Coating. United States: N. p., 2018.
Web. doi:10.1021/acs.langmuir.8b00181.
Ziemba, Christopher, Khavkin, Maria, Priftis, Dimitris, Acar, Handan, Mao, Jun, Benami, Maya, Gottlieb, Moshe, Tirrell, Matthew, Kaufman, Yair, & Herzberg, Moshe. Antifouling Properties of a Self-Assembling Glutamic Acid-Lysine Zwitterionic Polymer Surface Coating. United States. doi:https://doi.org/10.1021/acs.langmuir.8b00181
Ziemba, Christopher, Khavkin, Maria, Priftis, Dimitris, Acar, Handan, Mao, Jun, Benami, Maya, Gottlieb, Moshe, Tirrell, Matthew, Kaufman, Yair, and Herzberg, Moshe. Wed .
"Antifouling Properties of a Self-Assembling Glutamic Acid-Lysine Zwitterionic Polymer Surface Coating". United States. doi:https://doi.org/10.1021/acs.langmuir.8b00181. https://www.osti.gov/servlets/purl/1514874.
@article{osti_1514874,
title = {Antifouling Properties of a Self-Assembling Glutamic Acid-Lysine Zwitterionic Polymer Surface Coating},
author = {Ziemba, Christopher and Khavkin, Maria and Priftis, Dimitris and Acar, Handan and Mao, Jun and Benami, Maya and Gottlieb, Moshe and Tirrell, Matthew and Kaufman, Yair and Herzberg, Moshe},
abstractNote = {There is a necessity for the development of antifouling materials to resist adsorption of biomacromolecules. Here we describe the preparation of a novel zwitterionic block copolymer with the potential to prevent or delay the formation of microbial biofilms. The block copolymer comprised a zwitterionic (hydrophilic) section of alternating glutamic acid (negatively charged) and lysine (positively charged) units and a hydrophobic polystyrene section. Cryo-TEM and dynamic light-scattering (DLS) results showed that, on average, the block copolymer self-assembled into 7-nm-diameter micelles in aqueous solutions (0 to 100 mM NaCl, pH 6). Quartz crystal microbalance with dissipation monitoring (QCM-D), atomic force microscopy (AFM), and contact angle measurements demonstrated that the block copolymer self-assembled into a brush-like monolayer on polystyrene surfaces. The brush-like monolayer produced from a 100 mg/L block copolymer solution exhibited an average distance, d, of approximately 4-8 nm between each block copolymer molecule (center to center). Once the brush-like monolayer self-assembled, it reduced EPS adsorption onto the polystyrene surface by similar to 70% (mass), reduced the rate of bacterial attachment by >80%, and inhibited the development of thick biofilms. QCM-D results revealed that the EPS molecules penetrate between the chains of the brush and adsorb onto the polystyrene surface. Additionally, AFM analyses showed that the brush-like monolayer prevents the adhesion of large (>d) hydrophilic colloids onto the surface via hydration repulsion; yet, molecules or colloids small enough to fit between the brush polymers (},
doi = {10.1021/acs.langmuir.8b00181},
journal = {Langmuir},
number = 5,
volume = 35,
place = {United States},
year = {2018},
month = {4}
}
Web of Science
Figures / Tables:

Works referencing / citing this record:
Povidone-iodine-functionalized fluorinated copolymers with dual-functional antibacterial and antifouling activities
journal, January 2019
- Borjihan, Qinggele; Yang, Jiebing; Song, Qing
- Biomaterials Science, Vol. 7, Issue 8
Protein-resistant surface based on zwitterion-functionalized nanoparticles for marine antifouling applications
journal, January 2020
- Sun, Ding; Li, Peiyun; Li, Xiong
- New Journal of Chemistry, Vol. 44, Issue 5
Figures / Tables found in this record: