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Title: Antifouling Properties of a Self-Assembling Glutamic Acid-Lysine Zwitterionic Polymer Surface Coating

Abstract

There is a necessity for the development of antifouling materials to resist adsorption of biomacromolecules. Here we describe the preparation of a novel zwitterionic block copolymer with the potential to prevent or delay the formation of microbial biofilms. The block copolymer comprised a zwitterionic (hydrophilic) section of alternating glutamic acid (negatively charged) and lysine (positively charged) units and a hydrophobic polystyrene section. Cryo-TEM and dynamic light-scattering (DLS) results showed that, on average, the block copolymer self-assembled into 7-nm-diameter micelles in aqueous solutions (0 to 100 mM NaCl, pH 6). Quartz crystal microbalance with dissipation monitoring (QCM-D), atomic force microscopy (AFM), and contact angle measurements demonstrated that the block copolymer self-assembled into a brush-like monolayer on polystyrene surfaces. The brush-like monolayer produced from a 100 mg/L block copolymer solution exhibited an average distance, d, of approximately 4-8 nm between each block copolymer molecule (center to center). Once the brush-like monolayer self-assembled, it reduced EPS adsorption onto the polystyrene surface by similar to 70% (mass), reduced the rate of bacterial attachment by >80%, and inhibited the development of thick biofilms. QCM-D results revealed that the EPS molecules penetrate between the chains of the brush and adsorb onto the polystyrene surface. Additionally, AFMmore » analyses showed that the brush-like monolayer prevents the adhesion of large (>d) hydrophilic colloids onto the surface via hydration repulsion; yet, molecules or colloids small enough to fit between the brush polymers (« less

Authors:
 [1];  [1];  [2];  [2]; ORCiD logo [2];  [1];  [1];  [3];  [1]; ORCiD logo [1]
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  1. Ben-Gurion University of the Negev (BGU), Beersheba (Israel)
  2. Univ. of Chicago, IL (United States)
  3. Univ. of Chicago, IL (United States); Argonne National Lab. (ANL), Lemont, IL (United States)
Publication Date:
Research Org.:
Argonne National Lab. (ANL), Argonne, IL (United States)
Sponsoring Org.:
Ben-Gurion University of the Negev (BGU), Beersheba (Israel)
OSTI Identifier:
1514874
Grant/Contract Number:  
AC02-06CH11357
Resource Type:
Accepted Manuscript
Journal Name:
Langmuir
Additional Journal Information:
Journal Volume: 35; Journal Issue: 5; Journal ID: ISSN 0743-7463
Publisher:
American Chemical Society
Country of Publication:
United States
Language:
English
Subject:
37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY

Citation Formats

Ziemba, Christopher, Khavkin, Maria, Priftis, Dimitris, Acar, Handan, Mao, Jun, Benami, Maya, Gottlieb, Moshe, Tirrell, Matthew, Kaufman, Yair, and Herzberg, Moshe. Antifouling Properties of a Self-Assembling Glutamic Acid-Lysine Zwitterionic Polymer Surface Coating. United States: N. p., 2018. Web. doi:10.1021/acs.langmuir.8b00181.
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Ziemba, Christopher, Khavkin, Maria, Priftis, Dimitris, Acar, Handan, Mao, Jun, Benami, Maya, Gottlieb, Moshe, Tirrell, Matthew, Kaufman, Yair, & Herzberg, Moshe. Antifouling Properties of a Self-Assembling Glutamic Acid-Lysine Zwitterionic Polymer Surface Coating. United States. doi:https://doi.org/10.1021/acs.langmuir.8b00181
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Ziemba, Christopher, Khavkin, Maria, Priftis, Dimitris, Acar, Handan, Mao, Jun, Benami, Maya, Gottlieb, Moshe, Tirrell, Matthew, Kaufman, Yair, and Herzberg, Moshe. Wed . "Antifouling Properties of a Self-Assembling Glutamic Acid-Lysine Zwitterionic Polymer Surface Coating". United States. doi:https://doi.org/10.1021/acs.langmuir.8b00181. https://www.osti.gov/servlets/purl/1514874.
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@article{osti_1514874,
title = {Antifouling Properties of a Self-Assembling Glutamic Acid-Lysine Zwitterionic Polymer Surface Coating},
author = {Ziemba, Christopher and Khavkin, Maria and Priftis, Dimitris and Acar, Handan and Mao, Jun and Benami, Maya and Gottlieb, Moshe and Tirrell, Matthew and Kaufman, Yair and Herzberg, Moshe},
abstractNote = {There is a necessity for the development of antifouling materials to resist adsorption of biomacromolecules. Here we describe the preparation of a novel zwitterionic block copolymer with the potential to prevent or delay the formation of microbial biofilms. The block copolymer comprised a zwitterionic (hydrophilic) section of alternating glutamic acid (negatively charged) and lysine (positively charged) units and a hydrophobic polystyrene section. Cryo-TEM and dynamic light-scattering (DLS) results showed that, on average, the block copolymer self-assembled into 7-nm-diameter micelles in aqueous solutions (0 to 100 mM NaCl, pH 6). Quartz crystal microbalance with dissipation monitoring (QCM-D), atomic force microscopy (AFM), and contact angle measurements demonstrated that the block copolymer self-assembled into a brush-like monolayer on polystyrene surfaces. The brush-like monolayer produced from a 100 mg/L block copolymer solution exhibited an average distance, d, of approximately 4-8 nm between each block copolymer molecule (center to center). Once the brush-like monolayer self-assembled, it reduced EPS adsorption onto the polystyrene surface by similar to 70% (mass), reduced the rate of bacterial attachment by >80%, and inhibited the development of thick biofilms. QCM-D results revealed that the EPS molecules penetrate between the chains of the brush and adsorb onto the polystyrene surface. Additionally, AFM analyses showed that the brush-like monolayer prevents the adhesion of large (>d) hydrophilic colloids onto the surface via hydration repulsion; yet, molecules or colloids small enough to fit between the brush polymers (},
doi = {10.1021/acs.langmuir.8b00181},
journal = {Langmuir},
number = 5,
volume = 35,
place = {United States},
year = {2018},
month = {4}
}
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Journal Article:
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DOI:  https://doi.org/10.1021/acs.langmuir.8b00181
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Cited by: 4 works
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Figures / Tables:

Figure 1 Figure 1: Molecular schematic of the combination of hydrophobic polystyrene (2) to the hydrophilic peptide polymer (1) to form the zwitterionic peptide-polystyrene block copolymer (3).
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Works referencing / citing this record:

Povidone-iodine-functionalized fluorinated copolymers with dual-functional antibacterial and antifouling activities
journal, January 2019

  • Borjihan, Qinggele; Yang, Jiebing; Song, Qing
  • Biomaterials Science, Vol. 7, Issue 8
  • DOI: 10.1039/c9bm00583h

Protein-resistant surface based on zwitterion-functionalized nanoparticles for marine antifouling applications
journal, January 2020

  • Sun, Ding; Li, Peiyun; Li, Xiong
  • New Journal of Chemistry, Vol. 44, Issue 5
  • DOI: 10.1039/c9nj04266k
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