Antifouling Properties of a Self-Assembling Glutamic Acid-Lysine Zwitterionic Polymer Surface Coating

doi:10.1021/acs.langmuir.8b00181

Title: Antifouling Properties of a Self-Assembling Glutamic Acid-Lysine Zwitterionic Polymer Surface Coating

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Abstract

There is a necessity for the development of antifouling materials to resist adsorption of biomacromolecules. Here we describe the preparation of a novel zwitterionic block copolymer with the potential to prevent or delay the formation of microbial biofilms. The block copolymer comprised a zwitterionic (hydrophilic) section of alternating glutamic acid (negatively charged) and lysine (positively charged) units and a hydrophobic polystyrene section. Cryo-TEM and dynamic light-scattering (DLS) results showed that, on average, the block copolymer self-assembled into 7-nm-diameter micelles in aqueous solutions (0 to 100 mM NaCl, pH 6). Quartz crystal microbalance with dissipation monitoring (QCM-D), atomic force microscopy (AFM), and contact angle measurements demonstrated that the block copolymer self-assembled into a brush-like monolayer on polystyrene surfaces. The brush-like monolayer produced from a 100 mg/L block copolymer solution exhibited an average distance, d, of approximately 4-8 nm between each block copolymer molecule (center to center). Once the brush-like monolayer self-assembled, it reduced EPS adsorption onto the polystyrene surface by similar to 70% (mass), reduced the rate of bacterial attachment by >80%, and inhibited the development of thick biofilms. QCM-D results revealed that the EPS molecules penetrate between the chains of the brush and adsorb onto the polystyrene surface. Additionally, AFMmore »« less

Authors:

Ziemba, Christopher ^[1]; Khavkin, Maria ^[1]; Priftis, Dimitris ^[2]; Acar, Handan ^[2];

^[2]; Benami, Maya ^[1]; Gottlieb, Moshe ^[1]; Tirrell, Matthew ^[3]; Kaufman, Yair ^[1];

^[1]

Ben-Gurion University of the Negev (BGU), Beersheba (Israel)
Univ. of Chicago, IL (United States)
Univ. of Chicago, IL (United States); Argonne National Lab. (ANL), Lemont, IL (United States)

Publication Date:: 2018-04-11

Research Org.:: Argonne National Lab. (ANL), Argonne, IL (United States)

Sponsoring Org.:: Ben-Gurion University of the Negev (BGU), Beersheba (Israel)

OSTI Identifier:: 1514874

Grant/Contract Number:: [AC02-06CH11357]

Resource Type:: Accepted Manuscript

Journal Name:: Langmuir

Additional Journal Information:: [ Journal Volume: 35; Journal Issue: 5]; Journal ID: ISSN 0743-7463

Publisher:: American Chemical Society

Country of Publication:: United States

Language:: English

Subject:: 37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY

Citation Formats


                    Ziemba, Christopher, Khavkin, Maria, Priftis, Dimitris, Acar, Handan, Mao, Jun, Benami, Maya, Gottlieb, Moshe, Tirrell, Matthew, Kaufman, Yair, and Herzberg, Moshe. Antifouling Properties of a Self-Assembling Glutamic Acid-Lysine Zwitterionic Polymer Surface Coating.  United States: N. p., 2018. 
        Web.  doi:10.1021/acs.langmuir.8b00181.

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                    Ziemba, Christopher, Khavkin, Maria, Priftis, Dimitris, Acar, Handan, Mao, Jun, Benami, Maya, Gottlieb, Moshe, Tirrell, Matthew, Kaufman, Yair, & Herzberg, Moshe. Antifouling Properties of a Self-Assembling Glutamic Acid-Lysine Zwitterionic Polymer Surface Coating.  United States.  doi:10.1021/acs.langmuir.8b00181.

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                    Ziemba, Christopher, Khavkin, Maria, Priftis, Dimitris, Acar, Handan, Mao, Jun, Benami, Maya, Gottlieb, Moshe, Tirrell, Matthew, Kaufman, Yair, and Herzberg, Moshe. Wed .  
        "Antifouling Properties of a Self-Assembling Glutamic Acid-Lysine Zwitterionic Polymer Surface Coating".  United States.  doi:10.1021/acs.langmuir.8b00181.  https://www.osti.gov/servlets/purl/1514874.

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@article{osti_1514874,

  title        = {Antifouling Properties of a Self-Assembling Glutamic Acid-Lysine Zwitterionic Polymer Surface Coating},

  author       = {Ziemba, Christopher and Khavkin, Maria and Priftis, Dimitris and Acar, Handan and Mao, Jun and Benami, Maya and Gottlieb, Moshe and Tirrell, Matthew and Kaufman, Yair and Herzberg, Moshe},

  abstractNote = {There is a necessity for the development of antifouling materials to resist adsorption of biomacromolecules. Here we describe the preparation of a novel zwitterionic block copolymer with the potential to prevent or delay the formation of microbial biofilms. The block copolymer comprised a zwitterionic (hydrophilic) section of alternating glutamic acid (negatively charged) and lysine (positively charged) units and a hydrophobic polystyrene section. Cryo-TEM and dynamic light-scattering (DLS) results showed that, on average, the block copolymer self-assembled into 7-nm-diameter micelles in aqueous solutions (0 to 100 mM NaCl, pH 6). Quartz crystal microbalance with dissipation monitoring (QCM-D), atomic force microscopy (AFM), and contact angle measurements demonstrated that the block copolymer self-assembled into a brush-like monolayer on polystyrene surfaces. The brush-like monolayer produced from a 100 mg/L block copolymer solution exhibited an average distance, d, of approximately 4-8 nm between each block copolymer molecule (center to center). Once the brush-like monolayer self-assembled, it reduced EPS adsorption onto the polystyrene surface by similar to 70% (mass), reduced the rate of bacterial attachment by >80%, and inhibited the development of thick biofilms. QCM-D results revealed that the EPS molecules penetrate between the chains of the brush and adsorb onto the polystyrene surface. Additionally, AFM analyses showed that the brush-like monolayer prevents the adhesion of large (>d) hydrophilic colloids onto the surface via hydration repulsion; yet, molecules or colloids small enough to fit between the brush polymers (},

  doi          = {10.1021/acs.langmuir.8b00181},

  journal      = {Langmuir},

  number       = [5],

  volume       = [35],

  place        = {United States},

  year         = {2018},

  month        = {4}

}

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DOI: 10.1021/acs.langmuir.8b00181

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