Prosaposin is a biomarker of mesenchymal glioblastoma and regulates mesenchymal transition through the TGF‐β1/Smad signaling pathway

Jiang, Yang; Zhou, Jinpeng; Hou, Dianqi; Luo, Peng; Gao, Huiling; Ma, Yanju; Chen, Yin‐Sheng; Li, Long; Zou, Dan; Zhang, Haiying; Zhang, Ye; Jing, Zhitao

doi:10.1002/path.5278

Title: Prosaposin is a biomarker of mesenchymal glioblastoma and regulates mesenchymal transition through the TGF‐β1/Smad signaling pathway

Journal Article · 21 May 2019 · Journal of Pathology

DOI: https://doi.org/10.1002/path.5278 · OSTI ID:1514753

Jiang, Yang ^[1]; Zhou, Jinpeng ^[2]; Hou, Dianqi ^[3]; Luo, Peng ^[2]; Gao, Huiling ^[4]; Ma, Yanju ^[5]; Chen, Yin‐Sheng ^[6]; Li, Long ^[2]; Zou, Dan ^[7]; Zhang, Haiying ^[8]; Zhang, Ye ^[7];

^[2]

Department of Neurosurgery The First Hospital of China Medical University Shenyang City PR China, Department of Neurosurgery Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine Shanghai PR China
Department of Neurosurgery The First Hospital of China Medical University Shenyang City PR China
Department of Neurosurgery Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine Shanghai PR China
College of Life and Health Sciences, Northeastern University Shenyang PR China
Department of Medical Oncology Cancer Hospital of China Medical University Shenyang PR China
Department of Neurosurgery/Neuro‐oncology SunYat‐sen University Cancer Center, State Key Laboratory of Oncology in South China, Collaborative Innovation Center for Cancer Medicine Guangzhou PR China
The First laboratory of cancer institute, the First Hospital of China Medical University Shenyang City PR China
International Education College, Liaoning University of Traditional Chinese Medicine Shenyang City PR China

Abstract Mesenchymal glioblastoma (GBM) is the most aggressive subtype of GBM. Our previous study found that neurotrophic factor prosaposin (PSAP) is highly expressed and secreted in glioma and can promote the growth of glioma. The role of PSAP in mesenchymal GBM is still unclear. In this study, bioinformatic analysis, western blotting and RT‐qPCR were used to detect the expression of PSAP in different GBM subtypes. Human glioma cell lines and patient‐derived glioma stem cells were studied in vitro and in vivo , revealing that mesenchymal GBM expressed and secreted the highest level of PSAP among four subtypes of GBM, and PSAP could promote GBM invasion and epithelial–mesenchymal transition (EMT)‐like processes in vivo and in vitro . Bioinformatic analysis and western blotting showed that PSAP mainly played a regulatory role in GBM invasion and EMT‐like processes via the TGF‐β1/Smad signaling pathway. In conclusion, the overexpression and secretion of PSAP may be an important factor causing the high invasiveness of mesenchymal GBM. PSAP is therefore a potential target for the treatment of mesenchymal GBM. © 2019 Pathological Society of Great Britain and Ireland. Published by John Wiley & Sons, Ltd

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Sponsoring Organization:: USDOE

OSTI ID:: 1514753

Journal Information:: Journal of Pathology, Journal Name: Journal of Pathology Journal Issue: 1 Vol. 249; ISSN 0022-3417

Publisher:: Wiley Blackwell (John Wiley & Sons)Copyright Statement

Country of Publication:: United Kingdom

Language:: English

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