Pilot-scale hydrothermal pretreatment and optimized saccharification enables bisabolene production from multiple feedstocks

Pérez Pimienta, José A.; Papa, Gabriella; Rodriguez, Alberto; Barcelos, Carolina A.; Liang, Ling; Stavila, Vitalie; Sanchez, Arturo; Gladden, John M.; Simmons, Blake A.

doi:10.1039/C9GC00323A

Title: Pilot-scale hydrothermal pretreatment and optimized saccharification enables bisabolene production from multiple feedstocks

Abstract

Bisabolene bioconversion is demonstrated using pilot-scale hydrothermal pretreated biomass using four feedstocks with in-depth characterization analysis.

Authors:

^[1]; Papa, Gabriella ^[2];

^[2]; Barcelos, Carolina A. ^[2];

^[3];

^[4];

^[1];

^[2];

^[2]

Laboratorio de Futuros en Bioenergía, Unidad Guadalajara de Ingeniería Avanzada, Centro de Investigación y Estudios Avanzados (CINVESTAV), Zapopan, Mexico
Joint BioEnergy Institute, Biological Systems and Engineering Division, Lawrence Berkeley National Laboratory, Emeryville, USA
Advanced Biofuels Process Demonstration Unit, Lawrence Berkeley National Laboratory, Berkeley, USA
Energy Nanomaterials Department, Sandia National Laboratories, Livermore, USA

Publication Date:: 2019-06-04

Sponsoring Org.:: USDOE Office of Science (SC), Biological and Environmental Research (BER)

OSTI Identifier:: 1513310

Grant/Contract Number:: AC02-05CH11231

Resource Type:: Publisher's Accepted Manuscript

Journal Name:: Green Chemistry

Additional Journal Information:: Journal Name: Green Chemistry Journal Volume: 21 Journal Issue: 11; Journal ID: ISSN 1463-9262

Publisher:: Royal Society of Chemistry (RSC)

Country of Publication:: United Kingdom

Language:: English

Citation Formats


                    Pérez Pimienta, José A., Papa, Gabriella, Rodriguez, Alberto, Barcelos, Carolina A., Liang, Ling, Stavila, Vitalie, Sanchez, Arturo, Gladden, John M., and Simmons, Blake A. Pilot-scale hydrothermal pretreatment and optimized saccharification enables bisabolene production from multiple feedstocks.  United Kingdom: N. p., 2019. 
Web.  doi:10.1039/C9GC00323A.

Copy to clipboard


                    Pérez Pimienta, José A., Papa, Gabriella, Rodriguez, Alberto, Barcelos, Carolina A., Liang, Ling, Stavila, Vitalie, Sanchez, Arturo, Gladden, John M., & Simmons, Blake A. Pilot-scale hydrothermal pretreatment and optimized saccharification enables bisabolene production from multiple feedstocks.  United Kingdom.  https://doi.org/10.1039/C9GC00323A

Copy to clipboard


                    Pérez Pimienta, José A., Papa, Gabriella, Rodriguez, Alberto, Barcelos, Carolina A., Liang, Ling, Stavila, Vitalie, Sanchez, Arturo, Gladden, John M., and Simmons, Blake A. Tue .  
"Pilot-scale hydrothermal pretreatment and optimized saccharification enables bisabolene production from multiple feedstocks".  United Kingdom.  https://doi.org/10.1039/C9GC00323A.

Copy to clipboard


                    
@article{osti_1513310,

  title        = {Pilot-scale hydrothermal pretreatment and optimized saccharification enables bisabolene production from multiple feedstocks},

  author       = {Pérez Pimienta, José A. and Papa, Gabriella and Rodriguez, Alberto and Barcelos, Carolina A. and Liang, Ling and Stavila, Vitalie and Sanchez, Arturo and Gladden, John M. and Simmons, Blake A.},

  abstractNote = {Bisabolene bioconversion is demonstrated using pilot-scale hydrothermal pretreated biomass using four feedstocks with in-depth characterization analysis.},

  doi          = {10.1039/C9GC00323A},

  journal      = {Green Chemistry},

  number       = 11,

  volume       = 21,

  place        = {United Kingdom},

  year         = {2019},

  month        = {6}

}

Copy to clipboard

Journal Article:

Free Publicly Available Full Text

Accepted Manuscript (Publisher)

Publisher's Version of Record
https://doi.org/10.1039/C9GC00323A

Other availability

Search WorldCat to find libraries that may hold this journal

Citation Metrics:

Cited by: 1 work

Citation information provided by
Web of Science

Save / Share:

Export Metadata

Save to My Library

Works referenced in this record:

Comparison and optimization of enzymatic saccharification of soybean fibers recovered from aqueous extractions
journal, January 2011

Karki, Bishnu; Maurer, Devin; Kim, Tae Hyun
Bioresource Technology, Vol. 102, Issue 2
DOI: 10.1016/j.biortech.2010.08.004

Pilot scale pretreatment of wheat straw and comparative evaluation of commercial enzyme preparations for biomass saccharification and fermentation
journal, October 2015

Agrawal, Ruchi; Satlewal, Alok; Gaur, Ruchi
Biochemical Engineering Journal, Vol. 102
DOI: 10.1016/j.bej.2015.02.018

Effect of mechanical disruption on the effectiveness of three reactors used for dilute acid pretreatment of corn stover Part 2: morphological and structural substrate analysis
journal, January 2014

Ciesielski, Peter N.; Wang, Wei; Chen, Xiaowen
Biotechnology for Biofuels, Vol. 7, Issue 1
DOI: 10.1186/1754-6834-7-47

Assessing the molecular structure basis for biomass recalcitrance during dilute acid and hydrothermal pretreatments
journal, January 2013

Pu, Yunqiao; Hu, Fan; Huang, Fang
Biotechnology for Biofuels, Vol. 6, Issue 1
DOI: 10.1186/1754-6834-6-15

Current Understanding of the Correlation of Lignin Structure with Biomass Recalcitrance
journal, November 2016

Li, Mi; Pu, Yunqiao; Ragauskas, Arthur J.
Frontiers in Chemistry, Vol. 4
DOI: 10.3389/fchem.2016.00045

Jatropha cultivation in southern India: assessing farmers' experiences
journal, February 2012

Axelsson, Lisa; Franzén, Maria; Ostwald, Madelene
Biofuels, Bioproducts and Biorefining, Vol. 6, Issue 3
DOI: 10.1002/bbb.1324

Bioconversion of lignocellulose: inhibitors and detoxification
journal, January 2013

Jönsson, Leif J.; Alriksson, Björn; Nilvebrant, Nils-Olof
Biotechnology for Biofuels, Vol. 6, Issue 1
DOI: 10.1186/1754-6834-6-16

Pretreatment of lignocellulose: Formation of inhibitory by-products and strategies for minimizing their effects
journal, January 2016

Jönsson, Leif J.; Martín, Carlos
Bioresource Technology, Vol. 199
DOI: 10.1016/j.biortech.2015.10.009

Nanoscale Structure of the Cell Wall Protecting Cellulose from Enzyme Attack
journal, February 2011

Adani, Fabrizio; Papa, Gabriella; Schievano, Andrea
Environmental Science & Technology, Vol. 45, Issue 3
DOI: 10.1021/es1020263

Fuel ethanol production from lignocellulosic biomass: An overview on feedstocks and technological approaches
journal, December 2016

Zabed, H.; Sahu, J. N.; Boyce, A. N.
Renewable and Sustainable Energy Reviews, Vol. 66
DOI: 10.1016/j.rser.2016.08.038

Steam explosion pretreatment of softwood: the effect of the explosive decompression on enzymatic digestibility
journal, July 2016

Pielhop, Thomas; Amgarten, Janick; von Rohr, Philipp Rudolf
Biotechnology for Biofuels, Vol. 9, Issue 1
DOI: 10.1186/s13068-016-0567-1

Rapid Characterization of Woody Biomass Digestibility and Chemical Composition Using Near-infrared SpectroscopyFree Access
journal, November 2010

Hou, Shen; Li, Laigeng
Journal of Integrative Plant Biology, Vol. 53, Issue 2
DOI: 10.1111/j.1744-7909.2010.01003.x

Carotenoid-based phenotypic screen of the yeast deletion collection reveals new genes with roles in isoprenoid production
journal, January 2013

Özaydın, Bilge; Burd, Helcio; Lee, Taek Soon
Metabolic Engineering, Vol. 15
DOI: 10.1016/j.ymben.2012.07.010

Condensed lignin structures and re-localization achieved at high severities in autohydrolysis of Eucalyptus globulus wood and their relationship with cellulose accessibility : Autohydrolysis of
journal, April 2015

Araya, Fabio; Troncoso, Eduardo; Mendonça, Regis Teixeira
Biotechnology and Bioengineering, Vol. 112, Issue 9
DOI: 10.1002/bit.25604

Transition of Cellulose Crystalline Structure and Surface Morphology of Biomass as a Function of Ionic Liquid Pretreatment and Its Relation to Enzymatic Hydrolysis
journal, April 2011

Cheng, Gang; Varanasi, Patanjali; Li, Chenlin
Biomacromolecules, Vol. 12, Issue 4
DOI: 10.1021/bm101240z

HSQC-NMR analysis of lignin in woody (Eucalyptus globulus and Picea abies) and non-woody (Agave sisalana) ball-milled plant materials at the gel state 10th EWLP, Stockholm, Sweden, August 25–28, 2008
journal, November 2009

Rencoret, Jorge; Marques, Gisela; Gutiérrez, Ana
Holzforschung, Vol. 63, Issue 6
DOI: 10.1515/HF.2009.070

Characterization of agave bagasse as a function of ionic liquid pretreatment
journal, April 2015

Perez-Pimienta, Jose A.; Lopez-Ortega, Monica G.; Chavez-Carvayar, Jose A.
Biomass and Bioenergy, Vol. 75
DOI: 10.1016/j.biombioe.2015.02.026

Significant Differences in the Hydrolysis Behavior of Amorphous and Crystalline Portions within Microcrystalline Cellulose in Hot-Compressed Water
journal, April 2010

Yu, Yun; Wu, Hongwei
Industrial & Engineering Chemistry Research, Vol. 49, Issue 8
DOI: 10.1021/ie901925g

Identification and microbial production of a terpene-based advanced biofuel
journal, September 2011

Peralta-Yahya, Pamela P.; Ouellet, Mario; Chan, Rossana
Nature Communications, Vol. 2, Issue 1
DOI: 10.1038/ncomms1494

Pilot-scale cellulosic ethanol production using eucalyptus biomass pre-treated by dilute acid and steam explosion: Pilot-scale cellulosic ethanol production using eucalyptus biomass pretreated by dilute acid and steam explosion
journal, April 2016

McIntosh, Shane; Zhang, Zhanying; Palmer, Janice
Biofuels, Bioproducts and Biorefining, Vol. 10, Issue 4
DOI: 10.1002/bbb.1651

Energy production from biomass (part 1): overview of biomass
journal, May 2002

McKendry, Peter
Bioresource Technology, Vol. 83, Issue 1
DOI: 10.1016/S0960-8524(01)00118-3

Calorimetric evaluation indicates that lignin conversion to advanced biofuels is vital to improving energy yields
journal, January 2015

Gardner, James L.; He, Wei; Li, Chenlin
RSC Advances, Vol. 5, Issue 63
DOI: 10.1039/C5RA01503K

Optimization of the enzymatic hydrolysis conditions of steam-exploded wheat straw for maximum glucose and xylose recovery: Enzymatic hydrolysis of steam-exploded wheat straw
journal, April 2012

Marcos, Mónica; García-Cubero, María Teresa; González-Benito, Gerardo
Journal of Chemical Technology & Biotechnology, Vol. 88, Issue 2
DOI: 10.1002/jctb.3820

Exploring the effect of different plant lignin content and composition on ionic liquid pretreatment efficiency and enzymatic saccharification of Eucalyptus globulus L. mutants
journal, August 2012

Papa, G.; Varanasi, P.; Sun, L.
Bioresource Technology, Vol. 117
DOI: 10.1016/j.biortech.2012.04.065

Pre-processing of sugarcane bagasse for gasification in a downdraft biomass gasifier system: A comprehensive review
journal, December 2016

Anukam, Anthony; Mamphweli, Sampson; Reddy, Prashant
Renewable and Sustainable Energy Reviews, Vol. 66
DOI: 10.1016/j.rser.2016.08.046

Recalcitrance Assessment of the Agro-industrial Residues from Five Agave Species: Ionic Liquid Pretreatment, Saccharification and Structural Characterization
journal, May 2018

Pérez-Pimienta, José A.; Mojica-Álvarez, Reyna M.; Sánchez-Herrera, Leticia M.
BioEnergy Research, Vol. 11, Issue 3
DOI: 10.1007/s12155-018-9920-5

Co-extraction of soluble and insoluble sugars from energy sorghum based on a hydrothermal hydrolysis process
journal, December 2016

Yu, Qiang; Tan, Xuesong; Zhuang, Xinshu
Bioresource Technology, Vol. 221
DOI: 10.1016/j.biortech.2016.08.118

Characterization of Woody and Herbaceous Biomasses Lignin Composition with 1064 nm Dispersive Multichannel Raman Spectroscopy
journal, August 2012

Lupoi, Jason S.; Smith, Emily A.
Applied Spectroscopy, Vol. 66, Issue 8
DOI: 10.1366/12-06621

Composition of non-woody plant lignins and cinnamic acids by Py-GC/MS, Py/TMAH and FT-IR
journal, May 2007

del Río, José C.; Gutiérrez, Ana; Rodríguez, Isabel M.
Journal of Analytical and Applied Pyrolysis, Vol. 79, Issue 1-2
DOI: 10.1016/j.jaap.2006.09.003

Optimization of Dilute Acid Pretreatment and Enzymatic Hydrolysis of Phalaris aquatica L. Lignocellulosic Biomass in Batch and Fed-Batch Processes
journal, October 2016

Karapatsia, Anna; Pappas, Ioannis; Penloglou, Giannis
BioEnergy Research, Vol. 10, Issue 1
DOI: 10.1007/s12155-016-9793-4

Xylooligosaccharides production from lignocellulosic biomass using a pilot-scale pretreatment continuous tubular reactor. Modelling and experimental validation
journal, August 2019

Rodríguez, Felicia; Sanchez, Arturo; Amaya-Delgado, Lorena
Industrial Crops and Products, Vol. 134
DOI: 10.1016/j.indcrop.2019.03.058

Recent advances in second generation bioethanol production: An insight to pretreatment, saccharification and fermentation processes
journal, December 2017

Rastogi, Meenal; Shrivastava, Smriti
Renewable and Sustainable Energy Reviews, Vol. 80
DOI: 10.1016/j.rser.2017.05.225

Recent innovations in analytical methods for the qualitative and quantitative assessment of lignin
journal, September 2015

Lupoi, Jason S.; Singh, Seema; Parthasarathi, Ramakrishnan
Renewable and Sustainable Energy Reviews, Vol. 49
DOI: 10.1016/j.rser.2015.04.091

Characterization of Organosolv Lignins using Thermal and FT-IR Spectroscopic Analysis
journal, January 2013

Sammons, Rhea J.; Harper, David P.; Labbé, Nicole
BioResources, Vol. 8, Issue 2
DOI: 10.15376/biores.8.2.2752-2767

Optimization of Enzymatic Hydrolysis of Wheat Straw Pretreated by Alkaline Peroxide Using Response Surface Methodology
journal, August 2009

Qi, Benkun; Chen, Xiangrong; Shen, Fei
Industrial & Engineering Chemistry Research, Vol. 48, Issue 15
DOI: 10.1021/ie8016863

Demonstrating a separation-free process coupling ionic liquid pretreatment, saccharification, and fermentation with Rhodosporidium toruloides to produce advanced biofuels
journal, January 2018

Sundstrom, Eric; Yaegashi, Junko; Yan, Jipeng
Green Chemistry, Vol. 20, Issue 12
DOI: 10.1039/C8GC00518D

The role of pretreatment in improving the enzymatic hydrolysis of lignocellulosic materials
journal, January 2016

Sun, Shaoni; Sun, Shaolong; Cao, Xuefei
Bioresource Technology, Vol. 199
DOI: 10.1016/j.biortech.2015.08.061

Structural Characterization of Wheat Straw Lignin as Revealed by Analytical Pyrolysis, 2D-NMR, and Reductive Cleavage Methods
journal, December 2011

del Río, José C.; Rencoret, Jorge; Prinsen, Pepijn
Journal of Agricultural and Food Chemistry, Vol. 60, Issue 23
DOI: 10.1021/jf301002n

Evaluation of agave bagasse recalcitrance using AFEX™, autohydrolysis, and ionic liquid pretreatments
journal, July 2016

Perez-Pimienta, Jose A.; Flores-Gómez, Carlos A.; Ruiz, Héctor A.
Bioresource Technology, Vol. 211
DOI: 10.1016/j.biortech.2016.03.103

Similar Records in DOE PAGES and OSTI.GOV collections:

Sugar production from bioenergy sorghum by using pilot scale continuous hydrothermal pretreatment combined with disk refining

Journal Article Cheng, Ming-Hsun ; Dien, Bruce S. ; Lee, D. K. ; ... - Bioresource Technology

Chemical-free pretreatments are attracting increased interest because they generate less inhibitor in hydrolysates. In this study, pilot-scaled continuous hydrothermal (PCH) pretreatment followed by disk refining was evaluated and compared to laboratory-scale batch hot water (LHW) pretreatment. Bioenergy sorghum bagasse (BSB) was pretreated at 160–190 °C for 10 min with and without subsequent disk milling. Hydrothermal pretreatment and disk milling synergistically improved glucose and xylose release by 10–20% compared to hydrothermal pretreatment alone. Maximum yields of glucose and xylose of 82.55% and 70.78%, respectively were achieved, when BSB was pretreated at 190 °C and 180 °C followed by disk milling. LHWmore »« less
Cited by 3
https://doi.org/10.1016/j.biortech.2019.121663

Full Text Available
Impact of Pretreatment Technologies on Saccharification and Isopentenol Fermentation of Mixed Lignocellulosic Feedstocks

Journal Article Shi, Jian ; George, Kevin W. ; Sun, Ning ; ... - BioEnergy Research

In order to enable the large-scale production of biofuels or chemicals from lignocellulosic biomass, a consistent and affordable year-round supply of lignocellulosic feedstocks is essential. Feedstock blending and/or densification offers one promising solution to overcome current challenges on biomass supply, i.e., low energy and bulk densities and significant compositional variations. Therefore, it is imperative to develop conversion technologies that can process mixed pelleted biomass feedstocks with minimal negative impact in terms of overall performance of the relevant biorefinery unit operations: pretreatment, fermentable sugar production, and fuel titers. We processed the mixture of four feedstocks—corn stover, switchgrass, lodgepole pine, and eucalyptusmore »« less
https://doi.org/10.1007/s12155-015-9588-z
Demonstrating a separation-free process coupling ionic liquid pretreatment, saccharification, and fermentation with Rhodosporidium toruloides to produce advanced biofuels

Journal Article Sundstrom, Eric ; Yaegashi, Junko ; Yan, Jipeng ; ... - Green Chemistry

Achieving low cost and high efficiency lignocellulose deconstruction is a critical step towards widespread adoption of lignocellulosic biofuels. Certain ionic liquid (IL)-based pretreatment processes effectively reduce recalcitrance of lignocellulose to enzymatic degradation but require either costly separations following pretreatment or novel IL compatible processes to mitigate downstream toxicity. Here we demonstrate at benchtop and pilot bioreactor scales a separation-free, intensified process for IL pretreatment, saccharification, and fermentation of sorghum biomass to produce the sesquiterpene bisabolene, a precursor to the renewable diesel and jet fuel bisabolane. The deconstruction process employs the IL cholinium lysinate ([Ch][Lys]), followed by enzymatic saccharification with themore »« less
Cited by 8
https://doi.org/10.1039/C8GC00518D
Throughput, Reliability, and Yields of a Pilot-Scale Conversion Process for Production of Fermentable Sugars from Lignocellulosic Biomass: A Study on Feedstock Ash and Moisture

Journal Article Sievers, David A. ; Kuhn, Erik M. ; Thompson, Vicki S. ; ... - ACS Sustainable Chemistry & Engineering

Early lignocellulosic biorefineries have been plagued with many issues that involve feedstock handling problems and variations in conversion efficacy that stem from feedstock variability and complexity in dimensional, physical, chemical, and mechanical attributes. Feedstock ash and moisture content vary considerably in corn stover harvested from farms for bioconversion, and their effects on preprocessing (grinding/milling) and subsequent chemical and enzymatic conversion to fermentable sugars is systematically explored here using pilot-scale hammer mill grinders and chemical hydrolysis reactoris systematically explored. Corn stover with high ash content due to contamination from soil was found to (1) consume higher power during grinding and resultedmore »« less
Cited by 4
https://doi.org/10.1021/acssuschemeng.9b06550

Full Text Available
Pilot-Scale Biorefinery: Sustainable Transport Fuels from Biomass via Integrated Pyrolysis and Catalytic Hydroconversion - Wastewater Cleanup by Catalytic Hydrothermal Gasification

Technical Report Elliott, Douglas C. ; Olarte, Mariefel V. ; Hart, Todd R.

DOE-EE Bioenergy Technologies Office has set forth several goals to increase the use of bioenergy and bioproducts derived from renewable resources. One of these goals is to facilitate the implementation of the biorefinery. The biorefinery will include the production of liquid fuels, power and, in some cases, products. The integrated biorefinery should stand-alone from an economic perspective with fuels and power driving the economy of scale while the economics/profitability of the facility will be dependent on existing market conditions. UOP LLC proposed to demonstrate a fast pyrolysis based integrated biorefinery. Pacific Northwest National Laboratory (PNNL) has expertise in an importantmore »« less
https://doi.org/10.2172/1209865

Full Text Available

Similar Records

Title: Pilot-scale hydrothermal pretreatment and optimized saccharification enables bisabolene production from multiple feedstocks

Abstract

Citation Formats

Comparison and optimization of enzymatic saccharification of soybean fibers recovered from aqueous extractions journal, January 2011

Pilot scale pretreatment of wheat straw and comparative evaluation of commercial enzyme preparations for biomass saccharification and fermentation journal, October 2015

Effect of mechanical disruption on the effectiveness of three reactors used for dilute acid pretreatment of corn stover Part 2: morphological and structural substrate analysis journal, January 2014

Assessing the molecular structure basis for biomass recalcitrance during dilute acid and hydrothermal pretreatments journal, January 2013

Current Understanding of the Correlation of Lignin Structure with Biomass Recalcitrance journal, November 2016

Jatropha cultivation in southern India: assessing farmers' experiences journal, February 2012

Bioconversion of lignocellulose: inhibitors and detoxification journal, January 2013

Pretreatment of lignocellulose: Formation of inhibitory by-products and strategies for minimizing their effects journal, January 2016

Nanoscale Structure of the Cell Wall Protecting Cellulose from Enzyme Attack journal, February 2011

Fuel ethanol production from lignocellulosic biomass: An overview on feedstocks and technological approaches journal, December 2016

Steam explosion pretreatment of softwood: the effect of the explosive decompression on enzymatic digestibility journal, July 2016

Rapid Characterization of Woody Biomass Digestibility and Chemical Composition Using Near-infrared SpectroscopyFree Access journal, November 2010

Carotenoid-based phenotypic screen of the yeast deletion collection reveals new genes with roles in isoprenoid production journal, January 2013

Condensed lignin structures and re-localization achieved at high severities in autohydrolysis of Eucalyptus globulus wood and their relationship with cellulose accessibility : Autohydrolysis of journal, April 2015

Transition of Cellulose Crystalline Structure and Surface Morphology of Biomass as a Function of Ionic Liquid Pretreatment and Its Relation to Enzymatic Hydrolysis journal, April 2011

HSQC-NMR analysis of lignin in woody (Eucalyptus globulus and Picea abies) and non-woody (Agave sisalana) ball-milled plant materials at the gel state 10th EWLP, Stockholm, Sweden, August 25–28, 2008 journal, November 2009

Characterization of agave bagasse as a function of ionic liquid pretreatment journal, April 2015

Significant Differences in the Hydrolysis Behavior of Amorphous and Crystalline Portions within Microcrystalline Cellulose in Hot-Compressed Water journal, April 2010

Identification and microbial production of a terpene-based advanced biofuel journal, September 2011

Pilot-scale cellulosic ethanol production using eucalyptus biomass pre-treated by dilute acid and steam explosion: Pilot-scale cellulosic ethanol production using eucalyptus biomass pretreated by dilute acid and steam explosion journal, April 2016

Energy production from biomass (part 1): overview of biomass journal, May 2002

Calorimetric evaluation indicates that lignin conversion to advanced biofuels is vital to improving energy yields journal, January 2015

Optimization of the enzymatic hydrolysis conditions of steam-exploded wheat straw for maximum glucose and xylose recovery: Enzymatic hydrolysis of steam-exploded wheat straw journal, April 2012

Exploring the effect of different plant lignin content and composition on ionic liquid pretreatment efficiency and enzymatic saccharification of Eucalyptus globulus L. mutants journal, August 2012

Pre-processing of sugarcane bagasse for gasification in a downdraft biomass gasifier system: A comprehensive review journal, December 2016

Recalcitrance Assessment of the Agro-industrial Residues from Five Agave Species: Ionic Liquid Pretreatment, Saccharification and Structural Characterization journal, May 2018

Co-extraction of soluble and insoluble sugars from energy sorghum based on a hydrothermal hydrolysis process journal, December 2016

Characterization of Woody and Herbaceous Biomasses Lignin Composition with 1064 nm Dispersive Multichannel Raman Spectroscopy journal, August 2012

Composition of non-woody plant lignins and cinnamic acids by Py-GC/MS, Py/TMAH and FT-IR journal, May 2007

Optimization of Dilute Acid Pretreatment and Enzymatic Hydrolysis of Phalaris aquatica L. Lignocellulosic Biomass in Batch and Fed-Batch Processes journal, October 2016

Xylooligosaccharides production from lignocellulosic biomass using a pilot-scale pretreatment continuous tubular reactor. Modelling and experimental validation journal, August 2019

Recent advances in second generation bioethanol production: An insight to pretreatment, saccharification and fermentation processes journal, December 2017

Recent innovations in analytical methods for the qualitative and quantitative assessment of lignin journal, September 2015

Characterization of Organosolv Lignins using Thermal and FT-IR Spectroscopic Analysis journal, January 2013

Optimization of Enzymatic Hydrolysis of Wheat Straw Pretreated by Alkaline Peroxide Using Response Surface Methodology journal, August 2009

Demonstrating a separation-free process coupling ionic liquid pretreatment, saccharification, and fermentation with Rhodosporidium toruloides to produce advanced biofuels journal, January 2018

The role of pretreatment in improving the enzymatic hydrolysis of lignocellulosic materials journal, January 2016

Structural Characterization of Wheat Straw Lignin as Revealed by Analytical Pyrolysis, 2D-NMR, and Reductive Cleavage Methods journal, December 2011

Evaluation of agave bagasse recalcitrance using AFEX™, autohydrolysis, and ionic liquid pretreatments journal, July 2016

Comparison and optimization of enzymatic saccharification of soybean fibers recovered from aqueous extractions
journal, January 2011

Pilot scale pretreatment of wheat straw and comparative evaluation of commercial enzyme preparations for biomass saccharification and fermentation
journal, October 2015

Effect of mechanical disruption on the effectiveness of three reactors used for dilute acid pretreatment of corn stover Part 2: morphological and structural substrate analysis
journal, January 2014

Assessing the molecular structure basis for biomass recalcitrance during dilute acid and hydrothermal pretreatments
journal, January 2013

Current Understanding of the Correlation of Lignin Structure with Biomass Recalcitrance
journal, November 2016

Jatropha cultivation in southern India: assessing farmers' experiences
journal, February 2012

Bioconversion of lignocellulose: inhibitors and detoxification
journal, January 2013

Pretreatment of lignocellulose: Formation of inhibitory by-products and strategies for minimizing their effects
journal, January 2016

Nanoscale Structure of the Cell Wall Protecting Cellulose from Enzyme Attack
journal, February 2011

Fuel ethanol production from lignocellulosic biomass: An overview on feedstocks and technological approaches
journal, December 2016

Steam explosion pretreatment of softwood: the effect of the explosive decompression on enzymatic digestibility
journal, July 2016

Rapid Characterization of Woody Biomass Digestibility and Chemical Composition Using Near-infrared SpectroscopyFree Access
journal, November 2010

Carotenoid-based phenotypic screen of the yeast deletion collection reveals new genes with roles in isoprenoid production
journal, January 2013

Condensed lignin structures and re-localization achieved at high severities in autohydrolysis of Eucalyptus globulus wood and their relationship with cellulose accessibility : Autohydrolysis of
journal, April 2015

Transition of Cellulose Crystalline Structure and Surface Morphology of Biomass as a Function of Ionic Liquid Pretreatment and Its Relation to Enzymatic Hydrolysis
journal, April 2011

HSQC-NMR analysis of lignin in woody (Eucalyptus globulus and Picea abies) and non-woody (Agave sisalana) ball-milled plant materials at the gel state 10th EWLP, Stockholm, Sweden, August 25–28, 2008
journal, November 2009

Characterization of agave bagasse as a function of ionic liquid pretreatment
journal, April 2015

Significant Differences in the Hydrolysis Behavior of Amorphous and Crystalline Portions within Microcrystalline Cellulose in Hot-Compressed Water
journal, April 2010

Identification and microbial production of a terpene-based advanced biofuel
journal, September 2011

Pilot-scale cellulosic ethanol production using eucalyptus biomass pre-treated by dilute acid and steam explosion: Pilot-scale cellulosic ethanol production using eucalyptus biomass pretreated by dilute acid and steam explosion
journal, April 2016

Energy production from biomass (part 1): overview of biomass
journal, May 2002

Calorimetric evaluation indicates that lignin conversion to advanced biofuels is vital to improving energy yields
journal, January 2015

Optimization of the enzymatic hydrolysis conditions of steam-exploded wheat straw for maximum glucose and xylose recovery: Enzymatic hydrolysis of steam-exploded wheat straw
journal, April 2012

Exploring the effect of different plant lignin content and composition on ionic liquid pretreatment efficiency and enzymatic saccharification of Eucalyptus globulus L. mutants
journal, August 2012

Pre-processing of sugarcane bagasse for gasification in a downdraft biomass gasifier system: A comprehensive review
journal, December 2016

Recalcitrance Assessment of the Agro-industrial Residues from Five Agave Species: Ionic Liquid Pretreatment, Saccharification and Structural Characterization
journal, May 2018

Co-extraction of soluble and insoluble sugars from energy sorghum based on a hydrothermal hydrolysis process
journal, December 2016

Characterization of Woody and Herbaceous Biomasses Lignin Composition with 1064 nm Dispersive Multichannel Raman Spectroscopy
journal, August 2012

Composition of non-woody plant lignins and cinnamic acids by Py-GC/MS, Py/TMAH and FT-IR
journal, May 2007

Optimization of Dilute Acid Pretreatment and Enzymatic Hydrolysis of Phalaris aquatica L. Lignocellulosic Biomass in Batch and Fed-Batch Processes
journal, October 2016

Xylooligosaccharides production from lignocellulosic biomass using a pilot-scale pretreatment continuous tubular reactor. Modelling and experimental validation
journal, August 2019

Recent advances in second generation bioethanol production: An insight to pretreatment, saccharification and fermentation processes
journal, December 2017

Recent innovations in analytical methods for the qualitative and quantitative assessment of lignin
journal, September 2015

Characterization of Organosolv Lignins using Thermal and FT-IR Spectroscopic Analysis
journal, January 2013

Optimization of Enzymatic Hydrolysis of Wheat Straw Pretreated by Alkaline Peroxide Using Response Surface Methodology
journal, August 2009

Demonstrating a separation-free process coupling ionic liquid pretreatment, saccharification, and fermentation with Rhodosporidium toruloides to produce advanced biofuels
journal, January 2018

The role of pretreatment in improving the enzymatic hydrolysis of lignocellulosic materials
journal, January 2016

Structural Characterization of Wheat Straw Lignin as Revealed by Analytical Pyrolysis, 2D-NMR, and Reductive Cleavage Methods
journal, December 2011

Evaluation of agave bagasse recalcitrance using AFEX™, autohydrolysis, and ionic liquid pretreatments
journal, July 2016