skip to main content
DOE PAGES title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Microbial community structure and functions differ between native and novel (exotic-dominated) grassland ecosystems in an 8-year experiment

Abstract

Aims Grasslands dominated by non-native (exotic) species have replaced purely native-dominated areas in many parts of the world forming ‘novel’ ecosystems. It is still poorly understood how soil microbial communities and their functions are affected between these native- and exotic-dominated sites. Methods We sampled 64 experimental grassland plots in central Texas with plant species mixtures of either all native or all exotic species; half with summer irrigation. We tested how native vs. exotic plant species mixtures and summer irrigation affected bacterial and fungal community composition and structure, microbial phylotype co-occurrence, and rates of mineralization across sampling years. Results Plant origin significantly affected fungal community composition and structure, but not diversity, throughout the length of the study, while changes in bacterial communities were limited to certain years. Nitrogen and phosphorus mineralization rates were higher under native plant mixtures, and correlated with changes in particular fungal species. Microbial communities were more structured in exotic than native grassland plots, especially for the fungal community. Conclusions The results indicate that conversion of native to exotic grasslands will more strongly impact fungal than bacterial communities structure. Furthermore, these impacts can alter ecosystem functioning belowground via changes in nitrogen and phosphorus cycling.

Authors:
 [1]; ORCiD logo [2];  [3];  [2];  [4];  [2]
  1. Iowa State Univ., Ames, IA (United States); Washington State Univ., Pullman, WA (United States)
  2. Iowa State Univ., Ames, IA (United States)
  3. Iowa State Univ., Ames, IA (United States); Pacific Northwest National Lab. (PNNL), Richland, WA (United States)
  4. USDA-Agricultural Research Service, Temple, TX (United States)
Publication Date:
Research Org.:
Pacific Northwest National Lab. (PNNL), Richland, WA (United States)
Sponsoring Org.:
USDOE; National Science Foundation (NSF); USDA
OSTI Identifier:
1512861
Report Number(s):
PNNL-SA-133470
Journal ID: ISSN 0032-079X
Grant/Contract Number:  
AC05-76RL01830; 2014-67003-22067; DEB-0639417
Resource Type:
Accepted Manuscript
Journal Name:
Plant and Soil
Additional Journal Information:
Journal Volume: 432; Journal Issue: 1-2; Journal ID: ISSN 0032-079X
Publisher:
Springer
Country of Publication:
United States
Language:
English
Subject:
54 ENVIRONMENTAL SCIENCES; Grasslands; Exotic plants; Fungi; Bacteria; Microbial diversity; Mineralization; Neutral/niche processes

Citation Formats

Checinska Sielaff, Aleksandra, Upton, Racheal N., Hofmockel, Kirsten S., Xu, Xia, Polley, H. Wayne, and Wilsey, Brian J. Microbial community structure and functions differ between native and novel (exotic-dominated) grassland ecosystems in an 8-year experiment. United States: N. p., 2018. Web. doi:10.1007/s11104-018-3796-1.
Checinska Sielaff, Aleksandra, Upton, Racheal N., Hofmockel, Kirsten S., Xu, Xia, Polley, H. Wayne, & Wilsey, Brian J. Microbial community structure and functions differ between native and novel (exotic-dominated) grassland ecosystems in an 8-year experiment. United States. doi:10.1007/s11104-018-3796-1.
Checinska Sielaff, Aleksandra, Upton, Racheal N., Hofmockel, Kirsten S., Xu, Xia, Polley, H. Wayne, and Wilsey, Brian J. Mon . "Microbial community structure and functions differ between native and novel (exotic-dominated) grassland ecosystems in an 8-year experiment". United States. doi:10.1007/s11104-018-3796-1. https://www.osti.gov/servlets/purl/1512861.
@article{osti_1512861,
title = {Microbial community structure and functions differ between native and novel (exotic-dominated) grassland ecosystems in an 8-year experiment},
author = {Checinska Sielaff, Aleksandra and Upton, Racheal N. and Hofmockel, Kirsten S. and Xu, Xia and Polley, H. Wayne and Wilsey, Brian J.},
abstractNote = {Aims Grasslands dominated by non-native (exotic) species have replaced purely native-dominated areas in many parts of the world forming ‘novel’ ecosystems. It is still poorly understood how soil microbial communities and their functions are affected between these native- and exotic-dominated sites. Methods We sampled 64 experimental grassland plots in central Texas with plant species mixtures of either all native or all exotic species; half with summer irrigation. We tested how native vs. exotic plant species mixtures and summer irrigation affected bacterial and fungal community composition and structure, microbial phylotype co-occurrence, and rates of mineralization across sampling years. Results Plant origin significantly affected fungal community composition and structure, but not diversity, throughout the length of the study, while changes in bacterial communities were limited to certain years. Nitrogen and phosphorus mineralization rates were higher under native plant mixtures, and correlated with changes in particular fungal species. Microbial communities were more structured in exotic than native grassland plots, especially for the fungal community. Conclusions The results indicate that conversion of native to exotic grasslands will more strongly impact fungal than bacterial communities structure. Furthermore, these impacts can alter ecosystem functioning belowground via changes in nitrogen and phosphorus cycling.},
doi = {10.1007/s11104-018-3796-1},
journal = {Plant and Soil},
number = 1-2,
volume = 432,
place = {United States},
year = {2018},
month = {9}
}

Journal Article:
Free Publicly Available Full Text
Publisher's Version of Record

Citation Metrics:
Cited by: 2 works
Citation information provided by
Web of Science

Save / Share:

Works referenced in this record:

FUNGuild: An open annotation tool for parsing fungal community datasets by ecological guild
journal, April 2016


Impacts of climate change drivers on C4 grassland productivity: scaling driver effects through the plant community
journal, February 2014

  • Polley, H. W.; Derner, J. D.; Jackson, R. B.
  • Journal of Experimental Botany, Vol. 65, Issue 13
  • DOI: 10.1093/jxb/eru009

Exotic Plant Invasion Alters Nitrogen Dynamics in an arid Grassland
journal, October 2001


Fungal community responses to precipitation: FUNGAL CLIMATE RESPONSE
journal, October 2010


Plant invasions differentially affected by diversity and dominant species in native- and exotic-dominated grasslands
journal, November 2015

  • Xu, Xia; Polley, H. Wayne; Hofmockel, Kirsten
  • Ecology and Evolution, Vol. 5, Issue 23
  • DOI: 10.1002/ece3.1830

Novel ecosystems: implications for conservation and restoration
journal, November 2009

  • Hobbs, Richard J.; Higgs, Eric; Harris, James A.
  • Trends in Ecology & Evolution, Vol. 24, Issue 11
  • DOI: 10.1016/j.tree.2009.05.012

Comparison of Sequencing Utility Programs
journal, January 2013


Relative roles of niche and neutral processes in structuring a soil microbial community
journal, November 2009

  • Dumbrell, Alex J.; Nelson, Michaela; Helgason, Thorunn
  • The ISME Journal, Vol. 4, Issue 3
  • DOI: 10.1038/ismej.2009.122

Biodiversity, photosynthetic mode, and ecosystem services differ between native and novel ecosystems
journal, March 2014


Species composition but not diversity explains recovery from the 2011 drought in Texas grasslands
journal, March 2017

  • Xu, Xia; Polley, H. Wayne; Hofmockel, Kirsten
  • Ecosphere, Vol. 8, Issue 3
  • DOI: 10.1002/ecs2.1704

Invasive Plants Rapidly Reshape Soil Properties in a Grassland Ecosystem
journal, March 2017


Stochastic and deterministic assembly processes in subsurface microbial communities
journal, March 2012

  • Stegen, James C.; Lin, Xueju; Konopka, Allan E.
  • The ISME Journal, Vol. 6, Issue 9
  • DOI: 10.1038/ismej.2012.22

Selection on soil microbiomes reveals reproducible impacts on plant function
journal, October 2014

  • Panke-Buisse, Kevin; Poole, Angela C.; Goodrich, Julia K.
  • The ISME Journal, Vol. 9, Issue 4
  • DOI: 10.1038/ismej.2014.196

Global patterns of 16S rRNA diversity at a depth of millions of sequences per sample
journal, June 2010

  • Caporaso, J. G.; Lauber, C. L.; Walters, W. A.
  • Proceedings of the National Academy of Sciences, Vol. 108, Issue Supplement_1
  • DOI: 10.1073/pnas.1000080107

Quantifying the relative roles of selective and neutral processes in defining eukaryotic microbial communities
journal, March 2015

  • Morrison-Whittle, Peter; Goddard, Matthew R.
  • The ISME Journal, Vol. 9, Issue 9
  • DOI: 10.1038/ismej.2015.18

Belowground biodiversity and ecosystem functioning
journal, November 2014

  • Bardgett, Richard D.; van der Putten, Wim H.
  • Nature, Vol. 515, Issue 7528
  • DOI: 10.1038/nature13855

Biotic plant-soil feedbacks across temporal scales
journal, February 2013

  • Kardol, Paul; De Deyn, Gerlinde B.; Laliberté, Etienne
  • Journal of Ecology, Vol. 101, Issue 2
  • DOI: 10.1111/1365-2745.12046

Disentangling mechanisms that mediate the balance between stochastic and deterministic processes in microbial succession
journal, March 2015

  • Dini-Andreote, Francisco; Stegen, James C.; van Elsas, Jan Dirk
  • Proceedings of the National Academy of Sciences, Vol. 112, Issue 11
  • DOI: 10.1073/pnas.1414261112

Changes in fungal communities along a boreal forest soil fertility gradient
journal, May 2015

  • Sterkenburg, Erica; Bahr, Adam; Brandström Durling, Mikael
  • New Phytologist, Vol. 207, Issue 4
  • DOI: 10.1111/nph.13426

The importance of the microbiome of the plant holobiont
journal, February 2015

  • Vandenkoornhuyse, Philippe; Quaiser, Achim; Duhamel, Marie
  • New Phytologist, Vol. 206, Issue 4
  • DOI: 10.1111/nph.13312

Biodiversity, phenology and temporal niche differences between native- and novel exotic-dominated grasslands
journal, November 2011

  • Wilsey, Brian J.; Daneshgar, Pedram P.; Polley, H. Wayne
  • Perspectives in Plant Ecology, Evolution and Systematics, Vol. 13, Issue 4
  • DOI: 10.1016/j.ppees.2011.07.002

Patterns and Processes of Microbial Community Assembly
journal, September 2013

  • Nemergut, D. R.; Schmidt, S. K.; Fukami, T.
  • Microbiology and Molecular Biology Reviews, Vol. 77, Issue 3
  • DOI: 10.1128/MMBR.00051-12

Search and clustering orders of magnitude faster than BLAST
journal, August 2010


Invaded grassland communities have altered stability-maintenance mechanisms but equal stability compared to native communities
journal, December 2013

  • Wilsey, Brian J.; Daneshgar, Pedram P.; Hofmockel, Kirsten
  • Ecology Letters, Vol. 17, Issue 1
  • DOI: 10.1111/ele.12213

Novel ecosystems: theoretical and management aspects of the new ecological world order
journal, January 2006


UPARSE: highly accurate OTU sequences from microbial amplicon reads
journal, August 2013


Aboveground productivity and root–shoot allocation differ between native and introduced grass species
journal, August 2006


The SILVA ribosomal RNA gene database project: improved data processing and web-based tools
journal, November 2012

  • Quast, Christian; Pruesse, Elmar; Yilmaz, Pelin
  • Nucleic Acids Research, Vol. 41, Issue D1
  • DOI: 10.1093/nar/gks1219

Plant-soil feedbacks: the past, the present and future challenges
journal, February 2013

  • van der Putten, Wim H.; Bardgett, Richard D.; Bever, James D.
  • Journal of Ecology, Vol. 101, Issue 2
  • DOI: 10.1111/1365-2745.12054

Stagonospora and Septoria diseases of barley, oat, and rye
journal, December 2000


Atmospheric Warming and the Amplification of Precipitation Extremes
journal, August 2008


The checkerboard score and species distributions
journal, November 1990


Methodology for studying fluxes of soil mineral-N in situ
journal, January 1987


BLAST+: architecture and applications
journal, January 2009

  • Camacho, Christiam; Coulouris, George; Avagyan, Vahram
  • BMC Bioinformatics, Vol. 10, Issue 1
  • DOI: 10.1186/1471-2105-10-421

Effect of Rainfall-Induced Soil Geochemistry Dynamics on Grassland Soil Microbial Communities
journal, August 2012

  • Cruz-Martínez, Karelyn; Rosling, Anna; Zhang, Yang
  • Applied and Environmental Microbiology, Vol. 78, Issue 21
  • DOI: 10.1128/AEM.00203-12

Management of novel ecosystems: are novel approaches required?
journal, December 2008

  • Seastedt, Timothy R.; Hobbs, Richard J.; Suding, Katharine N.
  • Frontiers in Ecology and the Environment, Vol. 6, Issue 10
  • DOI: 10.1890/070046

Simple plant traits explain functional group diversity decline in novel grassland communities of Texas
journal, December 2012


Stagonospora nodorum: cause of stagonospora nodorum blotch of wheat
journal, May 2006


Nitrogen Mineralization: Challenges of a Changing Paradigm
journal, March 2004

  • Schimel, Joshua P.; Bennett, Jennifer
  • Ecology, Vol. 85, Issue 3
  • DOI: 10.1890/03-8002

The invasive annual cheatgrass increases nitrogen availability in 24-year-old replicated field plots
journal, October 2014


Despite strong seasonal responses, soil microbial consortia are more resilient to long-term changes in rainfall than overlying grassland
journal, March 2009

  • Cruz-Martínez, Karelyn; Suttle, K. Blake; Brodie, Eoin L.
  • The ISME Journal, Vol. 3, Issue 6
  • DOI: 10.1038/ismej.2009.16

The rhizosphere revisited: root microbiomics
journal, January 2013

  • Bakker, Peter A. H. M.; Berendsen, Roeland L.; Doornbos, Rogier F.
  • Frontiers in Plant Science, Vol. 4
  • DOI: 10.3389/fpls.2013.00165

Fungal Traits That Drive Ecosystem Dynamics on Land
journal, May 2015

  • Treseder, Kathleen K.; Lennon, Jay T.
  • Microbiology and Molecular Biology Reviews, Vol. 79, Issue 2
  • DOI: 10.1128/MMBR.00001-15

Ultra-high-throughput microbial community analysis on the Illumina HiSeq and MiSeq platforms
journal, March 2012

  • Caporaso, J. Gregory; Lauber, Christian L.; Walters, William A.
  • The ISME Journal, Vol. 6, Issue 8
  • DOI: 10.1038/ismej.2012.8

The Earth’s bounty: assessing and accessing soil microbial diversity
journal, October 1999


Greatest soil microbial diversity found in micro-habitats
journal, March 2018


Biotic Regulation of CO2 Uptake–Climate Responses: Links to Vegetation Properties
journal, June 2016


Long-Term Ecosystem Impacts of an Introduced Grass in the Northern Great Plains
journal, October 1999


Fast, scalable generation of high‐quality protein multiple sequence alignments using Clustal Omega
journal, January 2011

  • Sievers, Fabian; Wilm, Andreas; Dineen, David
  • Molecular Systems Biology, Vol. 7, Issue 1
  • DOI: 10.1038/msb.2011.75

    Works referencing / citing this record:

    Responses of Rhizospheric Microbial Communities of Native and Alien Plant Species to Cuscuta Parasitism
    journal, December 2019