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Title: Distinction of saffron cod ( Eleginus gracilis) from several other gadid species by using microsatellite markers

Abstract

Nine microsatellite loci isolated in saffron cod ( Eleginus gracilis) have potential applications for population genetics. Polymerase chain reaction products of samples of E. gracilis from northwestern Alaska amplified reliably, produced only one or two microsatellite bands, and had no apparent homozygote excess. A collection of E. gracilis sampled in the Gulf of Alaska (GOA) near Kodiak Island did not amplify reliably at one locus, and allele frequency profiles clustered distinctly (with principal component analysis [PCA]) from the northwestern Alaska collection. Northwestern Alaska and GOA E. gracilis collections were genetically different (on the basis of a standardized genetic differentiation measure [ G' ST]=0.313, chord distance [ D chord]=0.078, P<0.0001) and differed in expected aver-age heterozygosities at shared loci (0.859 and 0.689, respectively). We tested the microsatellite primers on other gadid species endemic to the northern Pacific Ocean, Bering Sea, and Arctic Ocean for cross-species amplification. Not all loci amplified reliably in navaga ( E. nawaga), Pacific tomcod ( Microgadus proximus), Arctic cod ( Boreogadus saida), Pacific cod ( Gadus macrocephalus), or walleye pollock ( G. chalcogrammus). Reliable loci varied in microsatellite size profiles and produced distinct PCA clusters and accurate genotype assignments that allowed accurate species identification. Furthermore the identificationsmore » support previous morphological and genetically determined systematic classifications and distinguished the geographically separated collections of E. gracilis.« less

Authors:
 [1];  [1];  [2];  [3];  [4];  [4];  [5];  [1];  [1]
  1. Univ. of Alaska Fairbanks, Juneau, AK (United States)
  2. NOAA, Seattle, WA (United States)
  3. Zoological Institute of the Russian Academy of Sciences, Saint Petersburg (Russia)
  4. Univ. of Georgia, Aiken, SC (United States)
  5. Univ. of Colorado School of Medicine, Aurora, CO (United States)
Publication Date:
Research Org.:
Univ. of Georgia, Athens, GA (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1512504
Grant/Contract Number:  
FC09-07SR22506
Resource Type:
Accepted Manuscript
Journal Name:
Fishery Bulletin
Additional Journal Information:
Journal Volume: 116; Journal Issue: 1; Journal ID: ISSN 0090-0656
Publisher:
NOAA Fisheries
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES

Citation Formats

Sme, Noel, Lyon, Sarah, Canino, Michael, Chernova, Natalia, O’Bryhim, Jason, Lance, Stacey, Jones, Kenneth, Mueter, Franz, and Gharrett, Anthony. Distinction of saffron cod (Eleginus gracilis) from several other gadid species by using microsatellite markers. United States: N. p., 2017. Web. doi:10.7755/fb.116.1.6.
Sme, Noel, Lyon, Sarah, Canino, Michael, Chernova, Natalia, O’Bryhim, Jason, Lance, Stacey, Jones, Kenneth, Mueter, Franz, & Gharrett, Anthony. Distinction of saffron cod (Eleginus gracilis) from several other gadid species by using microsatellite markers. United States. doi:10.7755/fb.116.1.6.
Sme, Noel, Lyon, Sarah, Canino, Michael, Chernova, Natalia, O’Bryhim, Jason, Lance, Stacey, Jones, Kenneth, Mueter, Franz, and Gharrett, Anthony. Wed . "Distinction of saffron cod (Eleginus gracilis) from several other gadid species by using microsatellite markers". United States. doi:10.7755/fb.116.1.6. https://www.osti.gov/servlets/purl/1512504.
@article{osti_1512504,
title = {Distinction of saffron cod (Eleginus gracilis) from several other gadid species by using microsatellite markers},
author = {Sme, Noel and Lyon, Sarah and Canino, Michael and Chernova, Natalia and O’Bryhim, Jason and Lance, Stacey and Jones, Kenneth and Mueter, Franz and Gharrett, Anthony},
abstractNote = {Nine microsatellite loci isolated in saffron cod (Eleginus gracilis) have potential applications for population genetics. Polymerase chain reaction products of samples of E. gracilis from northwestern Alaska amplified reliably, produced only one or two microsatellite bands, and had no apparent homozygote excess. A collection of E. gracilis sampled in the Gulf of Alaska (GOA) near Kodiak Island did not amplify reliably at one locus, and allele frequency profiles clustered distinctly (with principal component analysis [PCA]) from the northwestern Alaska collection. Northwestern Alaska and GOA E. gracilis collections were genetically different (on the basis of a standardized genetic differentiation measure [G'ST]=0.313, chord distance [Dchord]=0.078, P<0.0001) and differed in expected aver-age heterozygosities at shared loci (0.859 and 0.689, respectively). We tested the microsatellite primers on other gadid species endemic to the northern Pacific Ocean, Bering Sea, and Arctic Ocean for cross-species amplification. Not all loci amplified reliably in navaga (E. nawaga), Pacific tomcod (Microgadus proximus), Arctic cod (Boreogadus saida), Pacific cod (Gadus macrocephalus), or walleye pollock (G. chalcogrammus). Reliable loci varied in microsatellite size profiles and produced distinct PCA clusters and accurate genotype assignments that allowed accurate species identification. Furthermore the identifications support previous morphological and genetically determined systematic classifications and distinguished the geographically separated collections of E. gracilis.},
doi = {10.7755/fb.116.1.6},
journal = {Fishery Bulletin},
number = 1,
volume = 116,
place = {United States},
year = {2017},
month = {12}
}

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