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Title: Efficient Metabolic Exchange and Electron Transfer within a Syntrophic Trichloroethene-Degrading Coculture of Dehalococcoides mccartyi 195 and Syntrophomonas wolfei

Abstract

Dehalococcoides mccartyi195 (strain 195) andSyntrophomonas wolfeiwere grown in a sustainable syntrophic coculture using butyrate as an electron donor and carbon source and trichloroethene (TCE) as an electron acceptor. The maximum dechlorination rate (9.9 ± 0.1 μmol day-1) and cell yield [(1.1 ± 0.3) × 108cells μmol-1Cl-] of strain 195 maintained in coculture were, respectively, 2.6 and 1.6 times higher than those measured in the pure culture. The strain 195 cell concentration was about 16 times higher than that ofS. wolfeiin the coculture. Aqueous H2concentrations ranged from 24 to 180 nM during dechlorination and increased to 350 ± 20 nM when TCE was depleted, resulting in cessation of butyrate fermentation byS. wolfeiwith a theoretical Gibbs free energy of -13.7 ± 0.2 kJ mol -1. Carbon monoxide in the coculture was around 0.06 μmol per bottle, which was lower than that observed for strain 195 in isolation. The minimum H2 threshold value for TCE dechlorination by strain 195 in the coculture was 0.6 ± 0.1 nM. Cell aggregates during syntrophic growth were observed by scanning electron microscopy. The interspecies distances to achieve H2 fluxes required to support the measured dechlorination rates were predicted using Fick's law and demonstrated the need for aggregation.more » Filamentous appendages and extracellular polymeric substance (EPS)-like structures were present in the intercellular spaces. The transcriptome of strain 195 during exponential growth in the coculture indicated increased ATP-binding cassette transporter activities compared to the pure culture, while the membrane-bound energy metabolism related genes were expressed at stable levels.« less

Authors:
 [1];  [1];  [1];  [1];  [2]
  1. Univ. of California, Berkeley, CA (United States)
  2. Univ. of California, Berkeley, CA (United States); Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
Publication Date:
Research Org.:
Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
Sponsoring Org.:
USDOE Office of Science (SC)
OSTI Identifier:
1512089
Grant/Contract Number:  
AC02-05CH11231
Resource Type:
Accepted Manuscript
Journal Name:
Applied and Environmental Microbiology
Additional Journal Information:
Journal Volume: 81; Journal Issue: 6; Journal ID: ISSN 0099-2240
Publisher:
American Society for Microbiology
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES

Citation Formats

Mao, Xinwei, Stenuit, Benoit, Polasko, Alexandra, Alvarez-Cohen, Lisa, and Kostka, J. E. Efficient Metabolic Exchange and Electron Transfer within a Syntrophic Trichloroethene-Degrading Coculture of Dehalococcoides mccartyi 195 and Syntrophomonas wolfei. United States: N. p., 2015. Web. doi:10.1128/AEM.03464-14.
Mao, Xinwei, Stenuit, Benoit, Polasko, Alexandra, Alvarez-Cohen, Lisa, & Kostka, J. E. Efficient Metabolic Exchange and Electron Transfer within a Syntrophic Trichloroethene-Degrading Coculture of Dehalococcoides mccartyi 195 and Syntrophomonas wolfei. United States. doi:10.1128/AEM.03464-14.
Mao, Xinwei, Stenuit, Benoit, Polasko, Alexandra, Alvarez-Cohen, Lisa, and Kostka, J. E. Fri . "Efficient Metabolic Exchange and Electron Transfer within a Syntrophic Trichloroethene-Degrading Coculture of Dehalococcoides mccartyi 195 and Syntrophomonas wolfei". United States. doi:10.1128/AEM.03464-14. https://www.osti.gov/servlets/purl/1512089.
@article{osti_1512089,
title = {Efficient Metabolic Exchange and Electron Transfer within a Syntrophic Trichloroethene-Degrading Coculture of Dehalococcoides mccartyi 195 and Syntrophomonas wolfei},
author = {Mao, Xinwei and Stenuit, Benoit and Polasko, Alexandra and Alvarez-Cohen, Lisa and Kostka, J. E.},
abstractNote = {Dehalococcoides mccartyi195 (strain 195) andSyntrophomonas wolfeiwere grown in a sustainable syntrophic coculture using butyrate as an electron donor and carbon source and trichloroethene (TCE) as an electron acceptor. The maximum dechlorination rate (9.9 ± 0.1 μmol day-1) and cell yield [(1.1 ± 0.3) × 108cells μmol-1Cl-] of strain 195 maintained in coculture were, respectively, 2.6 and 1.6 times higher than those measured in the pure culture. The strain 195 cell concentration was about 16 times higher than that ofS. wolfeiin the coculture. Aqueous H2concentrations ranged from 24 to 180 nM during dechlorination and increased to 350 ± 20 nM when TCE was depleted, resulting in cessation of butyrate fermentation byS. wolfeiwith a theoretical Gibbs free energy of -13.7 ± 0.2 kJ mol -1. Carbon monoxide in the coculture was around 0.06 μmol per bottle, which was lower than that observed for strain 195 in isolation. The minimum H2 threshold value for TCE dechlorination by strain 195 in the coculture was 0.6 ± 0.1 nM. Cell aggregates during syntrophic growth were observed by scanning electron microscopy. The interspecies distances to achieve H2 fluxes required to support the measured dechlorination rates were predicted using Fick's law and demonstrated the need for aggregation. Filamentous appendages and extracellular polymeric substance (EPS)-like structures were present in the intercellular spaces. The transcriptome of strain 195 during exponential growth in the coculture indicated increased ATP-binding cassette transporter activities compared to the pure culture, while the membrane-bound energy metabolism related genes were expressed at stable levels.},
doi = {10.1128/AEM.03464-14},
journal = {Applied and Environmental Microbiology},
number = 6,
volume = 81,
place = {United States},
year = {2015},
month = {1}
}

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Cited by: 20 works
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Figures / Tables:

TABLE 1 TABLE 1: Stoichiometric energy generating reactions in the syntrophic coculture

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