Efficient Metabolic Exchange and Electron Transfer within a Syntrophic Trichloroethene-Degrading Coculture of Dehalococcoides mccartyi 195 and Syntrophomonas wolfei

Journal Article · 08 January 2015 · Applied and Environmental Microbiology

DOI: https://doi.org/10.1128/AEM.03464-14 · OSTI ID:1512089

Mao, Xinwei ^[1]; Stenuit, Benoit ^[1]; Polasko, Alexandra ^[1]; Alvarez-Cohen, Lisa ^[1]; Kostka, J. E. ^[2]

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1. Univ. of California, Berkeley, CA (United States)
2. Univ. of California, Berkeley, CA (United States); Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)

Dehalococcoides mccartyi195 (strain 195) andSyntrophomonas wolfeiwere grown in a sustainable syntrophic coculture using butyrate as an electron donor and carbon source and trichloroethene (TCE) as an electron acceptor. The maximum dechlorination rate (9.9 ± 0.1 μmol day^-1) and cell yield [(1.1 ± 0.3) × 10⁸cells μmol^-1Cl^-] of strain 195 maintained in coculture were, respectively, 2.6 and 1.6 times higher than those measured in the pure culture. The strain 195 cell concentration was about 16 times higher than that ofS. wolfeiin the coculture. Aqueous H₂concentrations ranged from 24 to 180 nM during dechlorination and increased to 350 ± 20 nM when TCE was depleted, resulting in cessation of butyrate fermentation byS. wolfeiwith a theoretical Gibbs free energy of -13.7 ± 0.2 kJ mol ^-1. Carbon monoxide in the coculture was around 0.06 μmol per bottle, which was lower than that observed for strain 195 in isolation. The minimum H₂ threshold value for TCE dechlorination by strain 195 in the coculture was 0.6 ± 0.1 nM. Cell aggregates during syntrophic growth were observed by scanning electron microscopy. The interspecies distances to achieve H₂ fluxes required to support the measured dechlorination rates were predicted using Fick's law and demonstrated the need for aggregation. Filamentous appendages and extracellular polymeric substance (EPS)-like structures were present in the intercellular spaces. The transcriptome of strain 195 during exponential growth in the coculture indicated increased ATP-binding cassette transporter activities compared to the pure culture, while the membrane-bound energy metabolism related genes were expressed at stable levels.

• Research Organization: Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)
• Sponsoring Organization: USDOE Office of Science (SC)
• Grant/Contract Number: AC02-05CH11231
• OSTI ID: 1512089
• Journal Information: Applied and Environmental Microbiology, Journal Name: Applied and Environmental Microbiology Journal Issue: 6 Vol. 81; ISSN 0099-2240
• Publisher: American Society for MicrobiologyCopyright Statement
• Country of Publication: United States
• Language: English