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Title: Light-Induced Reversible DNA Ligation of Gold Nanoparticle Superlattices

Abstract

DNA-mediated self-assembly of nanoparticles has been of great interest because it enables access to nanoparticle superstructures that cannot be synthesized otherwise. However, the programmability of higher order nanoparticle structures can be easily lost under DNA denaturing conditions. Here, we demonstrate that light can be employed as an external stimulus to master the stability of nanoparticle superlattices (SLs) via the promotion of a reversible photoligation of DNA in SLs. The oligonucleotides attached to the nanoparticles are encoded to ligate using 365 nm light, effectively locking the SLs and rendering them stable under DNA denaturing conditions. The reversible process of unlocking these structures is possible by irradiation with light at 315 nm, recovering the structures to their natural state. Our work inspires an alternative research direction toward postassembly manipulation of nanoparticle superstructures using external stimuli as a tool to enrich the library of additional material forms and their application in different media and environments.

Authors:
 [1]; ORCiD logo [2];  [3]; ORCiD logo [4]; ORCiD logo [5]; ORCiD logo [6]; ORCiD logo [7];  [8]; ORCiD logo [7]
  1. School of Physics and Astronomy, Faculty of Engineering and Physical Sciences, University of Southampton, Southampton, SO17 1BJ, U.K.
  2. Chemistry Research Laboratory, Department of Chemistry, University of Oxford, 12 Mansfield Road, Oxford, OX1 3TA, U.K., Chemistry Branch, Department of Science and Mathematics, Faculty of Petroleum and Mining Engineering, Suez University, Suez 43721, Egypt
  3. Center for Functional Nanomaterials, Brookhaven National Laboratory, Upton, New York 11973, United States, Department of Chemical Engineering, Columbia University, New York, New York 10027, United States
  4. School of Chemistry, Faculty of Engineering and Physical Sciences, University of Southampton, Southampton, SO17 1BJ, U.K.
  5. SPECIFIC-IKC, Materials Research Centre, College of Engineering, Swansea University, Bay Campus, Fabian Way, Swansea, SA1 8EN, U.K.
  6. Chemistry Research Laboratory, Department of Chemistry, University of Oxford, 12 Mansfield Road, Oxford, OX1 3TA, U.K.
  7. School of Physics and Astronomy, Faculty of Engineering and Physical Sciences, University of Southampton, Southampton, SO17 1BJ, U.K., Institute for Life Sciences, University of Southampton, Southampton, SO17 1BJ, U.K.
  8. Center for Functional Nanomaterials, Brookhaven National Laboratory, Upton, New York 11973, United States, Department of Chemical Engineering, Columbia University, New York, New York 10027, United States, Department of Applied Physics and Applied Mathematics, Columbia University, New York, New York 10027, United States
Publication Date:
Research Org.:
Univ. of Southampton (United Kingdom); Brookhaven National Lab. (BNL), Upton, NY (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Basic Energy Sciences (BES); USDOE Office of Science (SC), Basic Energy Sciences (BES) (SC-22). Materials Sciences & Engineering Division
OSTI Identifier:
1511760
Alternate Identifier(s):
OSTI ID: 1518952; OSTI ID: 1562481
Report Number(s):
BNL-212096-2019-JAAM
Journal ID: ISSN 1936-0851
Grant/Contract Number:  
SC0008772; SC0012704
Resource Type:
Published Article
Journal Name:
ACS Nano
Additional Journal Information:
Journal Name: ACS Nano Journal Volume: 13 Journal Issue: 5; Journal ID: ISSN 1936-0851
Publisher:
American Chemical Society
Country of Publication:
United States
Language:
English
Subject:
77 NANOSCIENCE AND NANOTECHNOLOGY; DNA; nanoparticles; photochemical ligation; self-assembly; superlattices

Citation Formats

De Fazio, Angela F., El-Sagheer, Afaf H., Kahn, Jason S., Nandhakumar, Iris, Burton, Matthew Richard, Brown, Tom, Muskens, Otto L., Gang, Oleg, and Kanaras, Antonios G. Light-Induced Reversible DNA Ligation of Gold Nanoparticle Superlattices. United States: N. p., 2019. Web. doi:10.1021/acsnano.9b01294.
De Fazio, Angela F., El-Sagheer, Afaf H., Kahn, Jason S., Nandhakumar, Iris, Burton, Matthew Richard, Brown, Tom, Muskens, Otto L., Gang, Oleg, & Kanaras, Antonios G. Light-Induced Reversible DNA Ligation of Gold Nanoparticle Superlattices. United States. doi:10.1021/acsnano.9b01294.
De Fazio, Angela F., El-Sagheer, Afaf H., Kahn, Jason S., Nandhakumar, Iris, Burton, Matthew Richard, Brown, Tom, Muskens, Otto L., Gang, Oleg, and Kanaras, Antonios G. Mon . "Light-Induced Reversible DNA Ligation of Gold Nanoparticle Superlattices". United States. doi:10.1021/acsnano.9b01294.
@article{osti_1511760,
title = {Light-Induced Reversible DNA Ligation of Gold Nanoparticle Superlattices},
author = {De Fazio, Angela F. and El-Sagheer, Afaf H. and Kahn, Jason S. and Nandhakumar, Iris and Burton, Matthew Richard and Brown, Tom and Muskens, Otto L. and Gang, Oleg and Kanaras, Antonios G.},
abstractNote = {DNA-mediated self-assembly of nanoparticles has been of great interest because it enables access to nanoparticle superstructures that cannot be synthesized otherwise. However, the programmability of higher order nanoparticle structures can be easily lost under DNA denaturing conditions. Here, we demonstrate that light can be employed as an external stimulus to master the stability of nanoparticle superlattices (SLs) via the promotion of a reversible photoligation of DNA in SLs. The oligonucleotides attached to the nanoparticles are encoded to ligate using 365 nm light, effectively locking the SLs and rendering them stable under DNA denaturing conditions. The reversible process of unlocking these structures is possible by irradiation with light at 315 nm, recovering the structures to their natural state. Our work inspires an alternative research direction toward postassembly manipulation of nanoparticle superstructures using external stimuli as a tool to enrich the library of additional material forms and their application in different media and environments.},
doi = {10.1021/acsnano.9b01294},
journal = {ACS Nano},
number = 5,
volume = 13,
place = {United States},
year = {2019},
month = {4}
}

Journal Article:
Free Publicly Available Full Text
Publisher's Version of Record
DOI: 10.1021/acsnano.9b01294

Citation Metrics:
Cited by: 4 works
Citation information provided by
Web of Science

Figures / Tables:

Scheme 1 Scheme 1: Reversible photochemical ligation of nanoparticle superlattices. Nanoparticles conjugated with oligonucleotides are hybridized under the appropriate conditions to form superlattices. One batch of oligonucleotide-coated nanoparticles contains a 3-cyanovinylcarbazole modification, which can react upon irradiation at λ = 365 nm with an adjacent thymine in the complementary strand, to formmore » an interstrand chemical bond. This photochemical process can be reversed upon irradiation with light at λ = 312 nm.« less

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Works referenced in this record:

Raw data for light induced reversible DNA ligation of gold nanoparticle superlattices
dataset, January 2019

  • Kanaras, Antonios; De Fazio, Angela, Federica; Muskens, Otto
  • University of Southampton
  • DOI: 10.5258/soton/d0860

Raw data for light induced reversible DNA ligation of gold nanoparticle superlattices
dataset, January 2019

  • Kanaras, Antonios; De Fazio, Angela, Federica; Muskens, Otto
  • University of Southampton
  • DOI: 10.5258/soton/d0860

    Works referencing / citing this record:

    Raw data for light induced reversible DNA ligation of gold nanoparticle superlattices
    dataset, January 2019

    • Kanaras, Antonios; De Fazio, Angela, Federica; Muskens, Otto
    • University of Southampton
    • DOI: 10.5258/soton/d0860

    Light‐Responsive Colloidal Crystals Engineered with DNA
    journal, January 2020


    DNA‐ and Field‐Mediated Assembly of Magnetic Nanoparticles into High‐Aspect Ratio Crystals
    journal, December 2019

    • Park, Sarah S.; Urbach, Zachary J.; Brisbois, Chase A.
    • Advanced Materials, Vol. 32, Issue 4
    • DOI: 10.1002/adma.201906626

      Figures/Tables have been extracted from DOE-funded journal article accepted manuscripts.